AB95065

Anti-FRYL antibody

Name: Anti-FRYL antibody
Brand: Abcam Limited
SKU: AB95065
Rating: 5 (3 reviews)

(3 리뷰들)

(3 제품이 사용된 논문 )

Rabbit Polyclonal FRYL antibody. Suitable for WB, IP and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human FRYL aa 2600 to C-terminus.

2 이미지

Unknown

Immunoprecipitation - Anti-FRYL antibody (AB95065)

Lane 1 : Detection of FRYL by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab95065 at 3 µg/mg lysate for IP.
Lane 2 : Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using an antibody which recognized an downstream epitope of FRYL.
Lane 3 : Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using Control IgG.

Subsequent Western blot detection used ab95065 at 1 µg/ml.

Developed using the ECL technique with an exposure time of 1 seconds.

All lanes:

Immunoprecipitation - Anti-FRYL antibody (ab95065)

Predicted band size: 340 kDa

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Western blot - Anti-FRYL antibody (AB95065)

All lanes:

Western blot - Anti-FRYL antibody (ab95065) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Lane 4:

293T cell lysate at 50 µg

Lane 5:

Mouse NIH3T3 cell lysate at 50 µg

Predicted band size: 340 kDa

true

Exposure time: 10s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human FRYL aa 2600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O94915

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-5 µg/mg of lysate", "IP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: Tris buffered saline, 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The FRYL protein also known as FYVE RhoGEF and PH domain-containing protein has a molecular mass of approximately 400 kDa. It is a member of the large family of proteins that contain FYVE domains which typically are involved in binding phosphatidylinositol 3-phosphate lipids. This protein expresses widely across tissues with notable levels found in the brain pancreas and kidney. FRYL's structure includes unique domains that facilitate its various functions within the cell.

Biological function summary

The FRYL protein engages in cellular functions such as cytoskeletal organization and membrane trafficking. It often participates in multiprotein complexes involving other proteins that regulate intracellular processes like cell movement and growth. Due to its domain composition FRYL interacts with lipids present in cellular membranes influencing the spatial arrangement of these membranes.

Pathways

The FRYL protein integrates into key cellular signaling pathways including the PI3K/AKT signaling pathway. This pathway plays a significant role in regulating cell survival and proliferation. FRYL interacts with other proteins such as Rho family GTPases which are essential for cytoskeletal dynamics and cellular architecture. Through these interactions FRYL indirectly connects to various signaling events within cells.

FRYL links to conditions such as cancer and neurological disorders. Abnormal expression or mutations of FRYL can lead to disrupted cellular processes that contribute to oncogenesis and neurodegenerative conditions. Connections with the protein AKT known for its involvement in cancer progression highlight FRYL's potential role in the development of these diseases. Understanding FRYL's functions offers insights into therapeutic targets for diseases affected by its dysregulation.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Plays a key role in maintaining the integrity of polarized cell extensions during morphogenesis, regulates the actin cytoskeleton and plays a key role in patterning sensory neuron dendritic fields by promoting avoidance between homologous dendrites as well as by limiting dendritic branching (By similarity). May function as a transcriptional activator.

See full target information FRYL

대체 명칭 보기

AF4P12, KIAA0826, FRYL, Protein furry homolog-like, ALL1-fused gene from chromosome 4p12 protein

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

mBio 10: PubMed31594818

2019

A Genome-Wide Knockout Screen in Human Macrophages Identified Host Factors Modulating Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Amy T Y Yeung,Yoon Ha Choi,Amy H Y Lee,Christine Hale,Hannes Ponstingl,Derek Pickard,David Goulding,Mark Thomas,Erin Gill,Jong Kyoung Kim,Allan Bradley,Robert E W Hancock,Gordon Dougan

Scientific reports 7:1334 PubMed28465505

2017

Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway.

Applications

Species

Mouse

Catarina Rippe,Baoyi Zhu,Katarzyna K Krawczyk,Ed Van Bavel,Sebastian Albinsson,Jonas Sjölund,Erik N T P Bakker,Karl Swärd

Environmental health perspectives 119:615-21 PubMed21147602

2010

Global gene expression analysis reveals dynamic and developmental stage-dependent enrichment of lead-induced neurological gene alterations.

Applications

Unspecified application

Species

Unspecified reactive species

Samuel M Peterson,Jun Zhang,Gregory Weber,Jennifer L Freeman

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-FRYL antibody

Immunoprecipitation - Anti-FRYL antibody (AB95065)

Western blot - Anti-FRYL antibody (AB95065)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise