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AB325857

Anti-FSHR antibody [HL2784] - BSA and Azide free

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Rabbit Recombinant Monoclonal FSH-R antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Mouse, Transfected cell lysate - Human, Human, Transfected cell line - Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human FSHR.
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Immunocytochemistry/ Immunofluorescence - Anti-FSHR antibody [HL2784] - BSA and Azide free (AB325857)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-FSHR antibody [HL2784] - BSA and Azide free (AB325857)

ab325857 detects FSH Receptor protein by immunofluorescent analysis. Sample : Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green : FSH Receptor stained by ab325857 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Western blot - Anti-FSHR antibody [HL2784] - BSA and Azide free (AB325857)
  • WB

Supplier Data

Western blot - Anti-FSHR antibody [HL2784] - BSA and Azide free (AB325857)

Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ab325857 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-FSHR antibody [HL2784] - BSA and Azide free (ab325857) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged FSHR transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged FSHR transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2784

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human FSHR.

P23945

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Transfected cell line - Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/5000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

타겟 정보

G protein-coupled receptor for follitropin, the follicle-stimulating hormone (PubMed : 11847099, PubMed : 24058690, PubMed : 24692546). Through cAMP production activates the downstream PI3K-AKT and ERK1/ERK2 signaling pathways (PubMed : 24058690).
See full target information FSHR

대체 명칭 보기

LGR1, FSHR, Follicle-stimulating hormone receptor, FSH-R, Follitropin receptor

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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