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AB281204

Anti-Furin antibody [EPR23363-111] - BSA and Azide free (Capture)

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Anti-Furin antibody [EPR23363-111] - BSA and Azide free (Capture) (ab281204) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting Furin in ELISA. Suitable for Human, Mouse, Rat.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency
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Sandwich ELISA - Anti-Furin antibody [EPR23363-111] - BSA and Azide free (Capture) (AB281204)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Furin antibody [EPR23363-111] - BSA and Azide free (Capture) (AB281204)

Example of Human Furin standard curve. Background-subtracted data values (mean +/- SD) are graphed.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23363-111

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB282292

Human Furin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Furin ELISA Kit (AB282292)

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Sample Prep & Detection Kits

AB102887

Glucose Oxidase Conjugation Kit - Lightning-Link®

Schematic Diagram - Glucose Oxidase Conjugation Kit - Lightning-Link® (AB102887)

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  • 0
Sample Prep & Detection Kits

AB188290

Gold-Maleimide Conjugation Kit (40nm, 20 OD)

Lateral flow assay - Gold-Maleimide Conjugation Kit (40nm, 20 OD) (AB188290)

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  • 0
Primary Antibodies

AB232826

Anti-SP17 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SP17 antibody (AB232826)

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Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Proteins & Peptides

AB290104

Recombinant Human Furin Protein

SDS-PAGE - Recombinant Human Furin Protein (AB290104)

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  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" }, "Rat": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab281204 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human Furin. The recommended pair for sandwich ELISA is:
Capture: ab281204, Human Furin Capture Antibody (unconjugated)
Detector: ab281054, Human Furin Detector Antibody (unconjugated)
The reference range value is 78 - 5000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Furin is a serine endoprotease that plays critical role in the cleavage of precursor proteins into their active forms. It belongs to the proprotein convertase family and is also known as furin protease. The molecular weight of furin is approximately 54 kDa. Furin is ubiquitously expressed in various tissues in the body with high expression levels in the liver and the central nervous system. Furin is mainly located in the trans-Golgi network where it processes many precursor proteins involved in various cellular functions.
Biological function summary

Furin processes precursor proteins by cleaving at specific sequence motifs. This activity is essential for the maturation of many hormones growth factors and receptors. Furin is not part of a larger complex; it functions independently in its proteolytic activity. The processing by furin ensures that proteins become functional affecting numerous biological processes across different cell types and tissues.

Pathways

The activity of furin impacts the regulation of the Notch signaling pathway and the MAPK/ERK pathway. In the Notch signaling pathway furin processes Notch receptors facilitating their activation and downstream signaling. In the MAPK/ERK pathway furin activates several key proteins including growth factors and receptors essential for cell proliferation and differentiation. Furin-related proteins such as convertase enzymes are part of a similar family having roles in overlapping or complementary pathways.

Furin is implicated in several conditions including cardiovascular diseases and certain cancers. In cardiovascular diseases furin's role in processing proteins such as pro-BNP may have implications in cardiac function and disease progression. In cancer altered furin activity can lead to the inappropriate activation of growth factors and receptors contributing to tumor progression. Furin inhibitors are researched for their potential to modulate furin activity in these diseases and provide therapeutic benefits.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Ubiquitous endoprotease within constitutive secretory pathways capable of cleavage at the RX(K/R)R consensus motif (PubMed : 11799113, PubMed : 1629222, PubMed : 1713771, PubMed : 2251280, PubMed : 24666235, PubMed : 25974265, PubMed : 7592877, PubMed : 7690548, PubMed : 9130696). Mediates processing of TGFB1, an essential step in TGF-beta-1 activation (PubMed : 7737999). Converts through proteolytic cleavage the non-functional Brain natriuretic factor prohormone into its active hormone BNP(1-32) (PubMed : 20489134, PubMed : 21763278). By mediating processing of accessory subunit ATP6AP1/Ac45 of the V-ATPase, regulates the acidification of dense-core secretory granules in islets of Langerhans cells (By similarity).. (Microbial infection) Cleaves and activates diphtheria toxin DT.. (Microbial infection) Cleaves and activates anthrax toxin protective antigen (PA).. (Microbial infection) Cleaves and activates HIV-1 virus Envelope glycoprotein gp160.. (Microbial infection) Required for H7N1 and H5N1 influenza virus infection probably by cleaving hemagglutinin.. (Microbial infection) Able to cleave S.pneumoniae serine-rich repeat protein PsrP.. (Microbial infection) Facilitates human coronaviruses EMC and SARS-CoV-2 infections by proteolytically cleaving the spike protein at the monobasic S1/S2 cleavage site. This cleavage is essential for spike protein-mediated cell-cell fusion and entry into human lung cells.. (Microbial infection) Facilitates mumps virus infection by proteolytically cleaving the viral fusion protein F.
See full target information FURIN

대체 명칭 보기

FUR, PACE, PCSK3, FURIN, Furin, Dibasic-processing enzyme, Paired basic amino acid residue-cleaving enzyme
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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