Anti-FURIN antibody [EPR28939-503]

• WB

Supplier Data

Western blot - Anti-FURIN antibody [EPR28939-503] (AB324231)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Negative control : LoVo (PMID : 20585585).

Low expression : IMR-32 and Jurkat.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-FURIN antibody [EPR28939-503] (ab324231) at 1/1000 dilution

Lane 1:

KARPAS-299 (human T cell lymphoma cell) whole cell lysate at 20 µg

Lane 2:

IMR-32 (human neuroblastoma neuroblast) whole cell lysate at 20 µg

Lane 3:

LoVo (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

Jurkat (human T cell leukemia T lymphocyte from peripheral blood) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 85 kDa,36 kDa

true

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-FURIN antibody [EPR28939-503] (AB324231)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : Daudi, Ramos (PMID : 7931173).

Low expression : Human lung.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-FURIN antibody [EPR28939-503] (ab324231) at 1/1000 dilution

Lane 1:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

HEK-293 (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 3:

K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate at 20 µg

Lane 4:

Daudi (human Burkitt's lymphoma lymphoblast) whole cell lysate at 20 µg

Lane 5:

Ramos (human Burkitt's lymphoma B lymphocyte) whole cell lysate at 20 µg

Lane 6:

Human placenta tissue lysate at 20 µg

Lane 7:

Human lung tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 85 kDa,36 kDa

false

Exposure time: 180s

• Dot

Supplier Data

Dot Blot - Anti-FURIN antibody [EPR28939-503] (AB324231)

Dot blot analysis of FURIN using ab324231 at 1 : 1000 (0.523 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Lane1 : No-tagged human PCSK3 recombinant protein
Lane2 : His-tagged human PCSK1 recombinant protein
Lane3 : His-tagged human PCSK4 recombinant protein
Lane4 : His-tagged human PCSK5 recombinant protein
Lane5 : His-tagged human PCSK6 recombinant protein

Exposure time : 180 seconds.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human PCSK1, PCSK4, PCSK5, and PCSK6.

All lanes:

Dot Blot - Anti-FURIN antibody [EPR28939-503] (ab324231) at 1/1000 dilution

Lane 1:

No-tagged human PCSK3 recombinant protein

Lane 2:

His-tagged human PCSK1 recombinant protein

Lane 3:

His-tagged human PCSK4 recombinant protein

Lane 4:

His-tagged human PCSK5 recombinant protein

Lane 5:

His-tagged human PCSK6 recombinant protein

Secondary

All lanes:

Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

false