AB243319

Anti-G-6-Pase antibody [CL5818]

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(3 제품이 사용된 논문 )

Mouse Monoclonal G-6-Pase antibody. Suitable for IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human G6PC1 aa 1-50.

대체 명칭 보기

G6PC, G6PT, G6PC1, Glucose-6-phosphatase catalytic subunit 1, Glucose-6-phosphatase, Glucose-6-phosphatase alpha, G-6-Pase, G6Pase, G6Pase-alpha

4 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)

Negative control :

Formalin-fixed, paraffin-embedded human tonsil tissue lymphoid cells stained for G-6-Pase using ab243319 at 1/5000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)

Formalin-fixed, paraffin-embedded human kidney tissue stained for G-6-Pase using ab243319 at 1/5000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)

Formalin-fixed, paraffin-embedded human duodenum tissue stained for G-6-Pase using ab243319 at 1/5000 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)

Formalin-fixed, paraffin-embedded human liver tissue stained for G-6-Pase using ab243319 at 1/5000 dilution in immunohistochemical analysis.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

CL5818

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human G6PC1 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

P35575

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/5000 - 1/10000", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

Purification 관련 사항

Purified from TCS.

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

G-6-Pase also known as glucose-6-phosphatase is an enzyme with a significant role in glucose metabolism. It has a molecular mass of approximately 36 kDa. The enzyme is primarily expressed in the liver and kidneys where it catalyzes the hydrolysis of glucose-6-phosphate into glucose and inorganic phosphate. This reaction is important in the final steps of gluconeogenesis and glycogenolysis processes essential for maintaining blood sugar levels. G-6-Pase is sometimes referred to as the catalytic subunit of the glucose-6-phosphatase complex and is integral for energy homeostasis.

Biological function summary

The enzyme is fundamental in regulating endogenous glucose production. Apart from its standalone activity G-6-Pase works in conjunction with other proteins as part of a larger glucose-6-phosphatase complex located in the endoplasmic reticulum membrane. It interacts with glucose-6-phosphate transporter proteins to efficiently manage glucose output. This complex interplay is important for meeting the organism's energy needs especially during fasted states when glucose supply from dietary intake is low.

Pathways

G-6-Pase plays a major role in gluconeogenesis and glycogenolysis. In gluconeogenesis it helps generate glucose from non-carbohydrate substrates important for energy balance during fasting or exercise. In glycogenolysis it aids in breaking down glycogen into glucose ensuring a constant energy supply. Within these pathways G-6-Pase associates with proteins like glycogen synthase and PEP carboxykinase which coordinate to regulate glucose homeostasis and energy production.

G-6-Pase is tightly linked to glycogen storage disease type I (GSD I) also known as von Gierke disease. This disorder results from a deficiency or dysfunction of the enzyme leading to impaired glucose release and severe hypoglycemia. Additionally misregulation of G-6-Pase activity is implicated in the development of type 2 diabetes where it affects insulin sensitivity and glucose output in the liver. G-6-Pase’s interaction with insulin receptor substrates highlights its connection to these metabolic diseases influencing their progression and severity.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Hydrolyzes glucose-6-phosphate to glucose in the endoplasmic reticulum. Forms with the glucose-6-phosphate transporter (SLC37A4/G6PT) the complex responsible for glucose production in the terminal step of glycogenolysis and gluconeogenesis. Hence, it is the key enzyme in homeostatic regulation of blood glucose levels.

See full target information G6PC1

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in physiology 13:844148 PubMed35264980

2022

Exogenous Linoleic Acid Intervention Alters Hepatic Glucose Metabolism in an Avian Embryo Model.

Applications

Unspecified application

Species

Unspecified reactive species

Xiufen Zhang,Qilin Wu,Wenxuan Zheng,Chuang Liu,Liang Huang,Xin Zuo,Wenquan Xiao,Xiaofeng Han,Hui Ye,Wence Wang,Yongwen Zhu,Lin Yang

Theranostics 11:7829-7843 PubMed34335967

2021

MicroRNA-185-5p inhibits hepatic gluconeogenesis and reduces fasting blood glucose levels by suppressing G6Pase.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Zheng,Jian Wan,Yi Shan,Xi Song,Jie Jin,Qing Su,Song Chen,Xinyuan Lu,Jialin Yang,Quanmin Li,Yuping Song,Bo Li

Journal of cellular and molecular medicine : PubMed32567187

2020

Large-scale transcriptome profiles reveal robust 20-signatures metabolic prediction models and novel role of G6PC in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wen-Hao Xu,Yue Xu,Xi Tian,Aihetaimujiang Anwaier,Wang-Rui Liu,Jun Wang,Wen-Kai Zhu,Da-Long Cao,Hong-Kai Wang,Guo-Hai Shi,Yuan-Yuan Qu,Hai-Liang Zhang,Ding-Wei Ye

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-G-6-Pase antibody [CL5818]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-G-6-Pase antibody [CL5818] (AB243319)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise