AB224214

Anti-GABA A Receptor alpha 3/GABRA3 antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal GABA A Receptor alpha 3/GABRA3 antibody. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GABRA3 aa 1-150.

대체 명칭 보기

Gamma-aminobutyric acid receptor subunit alpha-3, GABA(A) receptor subunit alpha-3, GABAAR subunit alpha-3, GABRA3

8 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Paraffin-embedded human cerebral cortex tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Paraffin-embedded human duodenum tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Paraffin-embedded mouse thalamus tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Paraffin-embedded mouse ventral pallidum tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Paraffin-embedded mouse olfactory bulb tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Paraffin-embedded mouse insular cortex tissue stained for GABA A Receptor alpha 3/GABRA3 using ab224214 at 1/500 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

Supplier Data

Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

All lanes:

Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab224214) at 1/250 dilution

All lanes:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate

Predicted band size: 55 kDa

true

Supplier Data

Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

All lanes:

Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (ab224214) at 1/250 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 55 kDa

true

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GABRA3 aa 1-150. The exact immunogen used to generate this antibody is proprietary information.

P34903

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500 - 1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed : 16412217, PubMed : 29053855). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (By similarity). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (PubMed : 16412217, PubMed : 29053855). Chloride influx into the postsynaptic neuron following GABAAR opening decreases the neuron ability to generate a new action potential, thereby reducing nerve transmission (PubMed : 16412217, PubMed : 29053855).

See full target information GABRA3

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of neural transmission (Vienna, Austria : 1996) : PubMed40886229

2025

The effect of recombinant botulinum neurotoxin A on neuropathic pain in the spared nerve injury mouse model.

Applications

Unspecified application

Species

Unspecified reactive species

Rasmus E Hammer,Akinyemi A Omoniyi,Mette Richner,Stephane Lezmi,Christian B Vaegter,Mikhail Kalinichev,Pall Karlsson,Jens R Nyengaard

PloS one 19:e0296024 PubMed38206981

2024

Expression of the cobalamin transporters cubam and MRP1 in the canine ileum-Upregulation in chronic inflammatory enteropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Stefanie Kather,Johannes Kacza,Helga Pfannkuche,Denny Böttcher,Chi-Hsuan Sung,Joerg M Steiner,Gotthold Gäbel,Franziska Dengler,Romy M Heilmann

Molecular brain 14:96 PubMed34174930

2021

Reductions in midbrain GABAergic and dopamine neuron markers are linked in schizophrenia.

Applications

Unspecified application

Species

Unspecified reactive species

Tertia D Purves-Tyson,Amelia M Brown,Christin Weissleder,Debora A Rothmond,Cynthia Shannon Weickert

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GABA A Receptor alpha 3/GABRA3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

Western blot - Anti-GABA A Receptor alpha 3/GABRA3 antibody (AB224214)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise