AB242001

Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24]

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(3 제품이 사용된 논문 )

Mouse Monoclonal GABA A Receptor alpha 5/GABRA5 antibody. Suitable for WB, ICC/IF and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GABRA5 aa 350-450.

대체 명칭 보기

Gamma-aminobutyric acid receptor subunit alpha-5, GABA(A) receptor subunit alpha-5, GABAAR subunit alpha-5, GABRA5

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24] (AB242001)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24] (AB242001)

Immunocytochemistry/Immunofluorescence analysis of Neuroblastoma cell line (SK-N-BE) with Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24] (ab242001). Fixation : 4% Formaldehyde for 15 min at RT. Primary Antibody : ab242001 at 1 : 100 for 60 min at RT. Secondary Antibody : Goat Anti-Mouse ATTO 488 at 1 : 100 for 60 min at RT. Counterstain : Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1 : 1000, 1 : 5000 for 60min RT, 5min RT. Localization : Cell Junction, Synapse, Postsynaptic Cell Membrane, Cell Membrane. Magnification : 60X.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24] (AB242001)

4% formaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells stained for GABA A Receptor alpha 5/GABRA5 (green) using ab242001 at 1/100 dilution for 1 hour at room temperature in ICC. Secondary antibody is Goat Anti-Mouse ATTO 488 at 1/100 dilution for 60 minutes at room temperature. Actin is detected with Phalloidin Texas Red F-Actin stain (red). The nuclear counterstain is DAPI (blue).

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

S415-24

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human GABRA5 aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

P31644

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

보관 버퍼

Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Alpha subunit of the heteropentameric ligand-gated chloride channel gated by gamma-aminobutyric acid (GABA), a major inhibitory neurotransmitter in the brain (PubMed : 14993607, PubMed : 29961870, PubMed : 30140029, PubMed : 31056671). GABA-gated chloride channels, also named GABA(A) receptors (GABAAR), consist of five subunits arranged around a central pore and contain GABA active binding site(s) located at the alpha and beta subunit interface(s) (PubMed : 30140029). When activated by GABA, GABAARs selectively allow the flow of chloride anions across the cell membrane down their electrochemical gradient (PubMed : 14993607, PubMed : 30140029). GABAARs containing alpha-5/GABRA5 subunits are mainly extrasynaptic and contribute to the tonic GABAergic inhibition in the hippocampus (By similarity). Extrasynaptic alpha-5-containing GABAARs in CA1 pyramidal neurons play a role in learning and memory processes (By similarity).

See full target information GABRA5

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Aging cell 23:e14209 PubMed38825816

2024

Sevoflurane-induced overexpression of extrasynaptic α5-GABAR via the RhoA/ROCK2 pathway impairs cognitive function in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Zhun Wang,Jinpeng Dong,Mengxue Zhang,Sixuan Wang,Jiangnan Wu,Shengran Wang,Yuan Luo,Yongan Wang,Yiqing Yin

Immunity, inflammation and disease 12:e1194 PubMed38501544

2024

Sodium butyrate activates the K channels to regulate the mechanism of Parkinson's disease microglia model inflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Ye Xu,Laofu Wen,Yunyi Tang,Zhenqiang Zhao,Miaojing Xu,Tan Wang,Zhibin Chen

Journal of neuroinflammation 18:204 PubMed34530841

2021

MD2 contributes to the pathogenesis of perioperative neurocognitive disorder via the regulation of α5GABA receptors in aged mice.

Applications

Unspecified application

Species

Unspecified reactive species

Wenqiang Zuo,Jianshuai Zhao,Jinming Zhang,Zongping Fang,Jiao Deng,Ze Fan,Yaru Guo,Jing Han,Wugang Hou,Hailong Dong,Feifei Xu,Lize Xiong

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24]

Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24] (AB242001)

Immunocytochemistry/ Immunofluorescence - Anti-GABA A Receptor alpha 5/GABRA5 antibody [S415-24] (AB242001)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise