Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (AB325669)

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum stained with ab325669 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield mounting medium. DAPI (blue) shows cell nuclei. The antibody stains neuropil granules identifying GABAergic synapses, Purkinje cell bodies, and the synaptic terminal of GABAergic interneurons in the molecular layer of the cerebellum. Magnification 400X.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (AB325669)

Representative section of formalin fixed, paraffin-embedded rat brain cerebellum stained with ab325669 at 1 : 100 dilution (green). The stained sections were mounted with Fluoroshield mounting medium. DAPI (blue) shows cell nuclei. The antibody stains neuropil granules identifying GABAergic synapses, Purkinje cell bodies, and the synaptic terminal of GABAergic interneurons in the molecular layer of the cerebellum. Magnification 100X.

• WB

Supplier Data

Western blot - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (AB325669)

Western blotting of rat brain homogenate with an anti-GAD65/67 mouse mAb and ab325669. The mouse mAb and ab325669 were detected with anti-mouse-HRP and anti-chicken-HRP, respectively. The antibody recognizes endogenous GAD1 with similar sensitivity as the anti-GAD65/67 mouse Mab in the rat brain homogenate at 65-67 kDa. GAD1 is an alternative name for GAD65/67.

All lanes:

Western blot - Anti-GAD1 antibody [L127/12] - Chicken IgY (Chimeric) (ab325669) at 10 µg/mL

All lanes:

rat brain homogenate

Secondary

All lanes:

anti-chicken-HRP

Observed band size: 65-67 kDa

false