AB236516

Anti-GADD34 antibody [OTI2B11]

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(4 제품이 사용된 논문 )

Mouse Monoclonal GADD34 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Mouse, Transfected cell line samples. Cited in 4 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PPP1R15A.

대체 명칭 보기

GADD34, PPP1R15A, Protein phosphatase 1 regulatory subunit 15A, Growth arrest and DNA damage-inducible protein GADD34, Myeloid differentiation primary response protein MyD116 homolog

6 이미지

Flow Cytometry (Intracellular) - Anti-GADD34 antibody [OTI2B11] (AB236516)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GADD34 antibody [OTI2B11] (AB236516)

Flow cytometric analysis of HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line transfected with either GADD34 overexpress plasmid (Red) or empty vector control plasmid (Blue) labeling GADD34 with ab236516 at 1/100 dilution.

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GADD34 antibody [OTI2B11] (AB236516)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling GADD34 with ab236516 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GADD34 antibody [OTI2B11] (AB236516)

Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling GADD34 with ab236516 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).

Supplier Data

Western blot - Anti-GADD34 antibody [OTI2B11] (AB236516)

All lanes:

Western blot - Anti-GADD34 antibody [OTI2B11] (ab236516) at 1/500 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate at 35 µg

Lane 3:

SVT2 cell lysate at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell lysate at 35 µg

Lane 5:

COS-7 (african green monkey kidney fibroblast-like cell line) cell lysate at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate at 35 µg

Lane 7:

MDCK (canine kidney cell line) cell lysate at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) cell lysate at 35 µg

Predicted band size: 73 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-GADD34 antibody [OTI2B11] (AB236516)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GADD34 antibody [OTI2B11] (AB236516)

COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY GADD34 stained for GADD34 (green) using ab236516 at 1/100 dilution in ICC/IF.

Supplier Data

Western blot - Anti-GADD34 antibody [OTI2B11] (AB236516)

All lanes:

Western blot - Anti-GADD34 antibody [OTI2B11] (ab236516) at 1/500 dilution

Lane 1:

pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate at 5 µg

Lane 2:

pCMV6-ENTRY GADD34 cDNA transfected HEK-293T cell lysate at 5 µg

Predicted band size: 73 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2B11

Isotype

IgG1

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PPP1R15A.

O75807

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Transfected cell line": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Clone OTI2B11 (formerly 2B11).

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

Purification 관련 사항

Purified from cell culture supernatant.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Recruits the serine/threonine-protein phosphatase PPP1CA to prevents excessive phosphorylation of the translation initiation factor eIF-2A/EIF2S1, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress (PubMed : 26095357, PubMed : 26742780). Down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 by PP1 (PubMed : 14718519). May promote apoptosis by inducing p53/TP53 phosphorylation on 'Ser-15' (PubMed : 14635196). Plays an essential role in autophagy by tuning translation during starvation, thus enabling lysosomal biogenesis and a sustained autophagic flux (PubMed : 32978159). Also acts a viral restriction factor by attenuating HIV-1 replication (PubMed : 31778897). Mechanistically, mediates the inhibition of HIV-1 TAR RNA-mediated translation (PubMed : 31778897).. (Microbial infection) Promotes enterovirus 71 replication by mediating the internal ribosome entry site (IRES) activity of viral 5'-UTR.

See full target information PPP1R15A

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of gastrointestinal oncology 14:1669-1693 PubMed37720449

2023

Esophageal cancer stem cells reduce hypoxia-induced apoptosis by inhibiting the GRP78-perk-eIF2α-ATF4-CHOP pathway .

Applications

Unspecified application

Species

Unspecified reactive species

Ruijiang Lin,Minjie Ma,Biao Han,Ya Zheng,Yuping Wang,Yongning Zhou

CNS neuroscience & therapeutics 29:1049-1066 PubMed36650674

2023

Human-induced pluripotent stem cell-derived cerebral organoid of leukoencephalopathy with vanishing white matter.

Applications

Unspecified application

Species

Unspecified reactive species

Jiong Deng,Jie Zhang,Kai Gao,Ling Zhou,Yuwu Jiang,Jingmin Wang,Ye Wu

Bioengineered 13:7894-7903 PubMed35291928

2022

Montelukast ameliorated pemetrexed-induced cytotoxicity in hepatocytes by mitigating endoplasmic reticulum (ER) stress and nucleotide oligomerization domain-like receptor protein 3 (NLRP3) activation.

Applications

Unspecified application

Species

Unspecified reactive species

Zhengdong Fei,Lu Zhang,Lei Wang,Hui Jiang,Aiqin Peng

Cell death & disease 13:9 PubMed34930892

2021

CDK5RAP2 loss-of-function causes premature cell senescence via the GSK3β/β-catenin-WIP1 pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xidi Wang,Patrick Sipila,Zizhen Si,Jesusa L Rosales,Xu Gao,Ki-Young Lee

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GADD34 antibody [OTI2B11]

Flow Cytometry (Intracellular) - Anti-GADD34 antibody [OTI2B11] (AB236516)

Flow Cytometry (Intracellular) - Anti-GADD34 antibody [OTI2B11] (AB236516)

Flow Cytometry (Intracellular) - Anti-GADD34 antibody [OTI2B11] (AB236516)

Western blot - Anti-GADD34 antibody [OTI2B11] (AB236516)

Immunocytochemistry/ Immunofluorescence - Anti-GADD34 antibody [OTI2B11] (AB236516)

Western blot - Anti-GADD34 antibody [OTI2B11] (AB236516)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise