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AB135398

Anti-GAL4 antibody [14-7E10G10]

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(3 제품이 사용된 논문 )

Mouse Monoclonal GAL4 antibody. Suitable for WB and reacts with Saccharomyces cerevisiae samples. Cited in 3 publications.

대체 명칭 보기

YPL248C, GAL4, Regulatory protein GAL4, GAL81, Gal4p, Regulatory protein GAL4

1 이미지
Western blot - Anti-GAL4 antibody [14-7E10G10] (AB135398)
  • WB

Unknown

Western blot - Anti-GAL4 antibody [14-7E10G10] (AB135398)

All lanes:

Western blot - Anti-GAL4 antibody [14-7E10G10] (ab135398) at 0.5 µg/mL

Lane 1:

Whole cell yeast lysate control from untransformed SEY6210 pep4-3 strain (a kind gift from Prof. Tom Stevens, University of Oregon).

Lane 2:

Whole cell yeast lysate from SEY6210 pep4-3 strain transformed with pJK22-pGBDU plasmid containing GAL4 DNA Binding domain fused to the VPS60 Gene (a kind gift from Prof. Tom Stevens, University of Oregon).

Lane 3:

Whole cell yeast lysate from SEY6210 pep4-3 strain transformed with pJk23-pGAD plasmid containing GAL4 Active Domain fused to the VPS60 Gene (a kind gift from Prof. Tom Stevens, University of Oregon).

Secondary

Lane 1:

Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) - 1:10000

Lane 2:

Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP)- 1:10000

Lane 3:

Goat polyclonal to Mouse IgG – H&L – Pre-Adsorbed (HRP) - 1:10000

Predicted band size: 99 kDa

Observed band size: 56 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

14-7E10G10

Isotype

IgG2b

Carrier free

No

Reacts with

Saccharomyces cerevisiae

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB229347

Anti-GAL4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody (AB229347)

  • 0
  • 0
Primary Antibodies

AB175185

Anti-GAL4 antibody [EPR12710(B)]

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] (AB175185)

  • 0
  • 0
Primary Antibodies

AB241394

Anti-GAL4 antibody [EPR12710(B)] - BSA and Azide free

RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody [EPR12710(B)] - BSA and Azide free (AB241394)

  • 0
  • 0
Primary Antibodies

AB135397

Anti-GAL4 antibody [15-6E10A7]

Western blot - Anti-GAL4 antibody [15-6E10A7] (AB135397)

  • 0
  • 0
Primary Antibodies

AB154309

Anti-GAL4 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAL4 antibody (AB154309)

  • 0
  • 0

보완 제품

Primary Antibodies

AB14955

Anti-Histone H3 (phospho S10) antibody [mAbcam 14955]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Histone H3 (phospho S10) antibody [mAbcam 14955] (AB14955)

  • 4
  • 26
Primary Antibodies

AB1791

Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade

Lab Essentials

ChIP - Anti-Histone H3 antibody - Nuclear Marker and ChIP Grade (AB1791)

  • 5
  • 233
Primary Antibodies

AB207305

Anti-SQSTM1 / p62 antibody [EPR18351] - Autophagosome Marker

20ul selling size|KO Validated|RabMAb|Recombinant|Lab Essentials|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-SQSTM1 / p62 antibody [EPR18351] - Autophagosome Marker (AB207305)

  • 5
  • 3
Primary Antibodies

AB281588

Anti-MAP2 antibody [RM1010] - Neuronal Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Flow Cytometry (Intracellular) - Anti-MAP2 antibody [RM1010] - Neuronal Marker (AB281588)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>" } } }
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제품 세부 정보

The GAL4 Activation Domain monoclonal antibody binds specifically to the AD of the yeast GAL4 protein. This antibody is useful for verifying the expression and correct molecular weight of fusion proteins containing GAL4 AD as the fusion partner in two hybrid studies. The activation domain is typically used in a two-hybrid experiment as the fusion partner for a library of cDNA clones to screen against a protein of interest fused to a DNA-binding domain. Gal4is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Precipitation Ammonium Sulphate
보관 버퍼
pH: 7.5 Preservative: 0.02% Sodium azide
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.
See full target information GAL4
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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

mSystems 9:e0100424 PubMed39526800

2024

A comprehensive two-hybrid analysis to explore the effector-effector interactome.

Applications

Unspecified application

Species

Unspecified reactive species

Harley O'Connor Mount,Malene L Urbanus,Dayag Sheykhkarimli,Atina G Coté,Florent Laval,Georges Coppin,Nishka Kishore,Roujia Li,Kerstin Spirohn-Fitzgerald,Morgan O Petersen,Jennifer J Knapp,Dae-Kyum Kim,Jean-Claude Twizere,Michael A Calderwood,Marc Vidal,Frederick P Roth,Alexander W Ensminger

PLoS pathogens 20:e1012662 PubMed39514589

2024

Use of the Puccinia sorghi haustorial transcriptome to identify and characterize AvrRp1-D recognized by the maize Rp1-D resistance protein.

Applications

Unspecified application

Species

Unspecified reactive species

Saet-Byul Kim,Ki-Tae Kim,Solhee In,Namrata Jaiswal,Gir-Won Lee,Seungmee Jung,Abigail Rogers,Libia F Gómez-Trejo,Sujan Gautam,Matthew Helm,Hee-Kyung Ahn,Hye-Young Lee,Quentin D Read,Jongchan Woo,Katerina L Holan,Steven A Whitham,Jonathan D G Jones,Doil Choi,Ralph Dean,Eunsook Park,Peter Balint-Kurti

mBio 15:e0322123 PubMed38335095

2024

An acetyltransferase effector conserved across species targets the eukaryotic eIF3 complex to modulate protein translation.

Applications

Unspecified application

Species

Unspecified reactive species

Lukas Syriste,Deepak T Patel,Peter J Stogios,Tatiana Skarina,Dhruvin Patel,Alexei Savchenko
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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