AB10482

Anti-Galectin 7 antibody

Name: Anti-Galectin 7 antibody
Brand: Abcam Limited
SKU: AB10482
Rating: 5 (5 reviews)

(5 리뷰들)

(9 제품이 사용된 논문 )

Rabbit Polyclonal Galectin 7 antibody. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Mouse, Human samples. Cited in 9 publications. Immunogen corresponding to Synthetic Peptide within Human LGALS7 aa 100 to C-terminus.

대체 명칭 보기

PIG1, LGALS7B, LGALS7, Galectin-7, Gal-7, HKL-14, PI7, p53-induced gene 1 protein

1 이미지

Unknown

Western blot - Anti-Galectin 7 antibody (AB10482)

ab10481 detection of mouse galectin 7 by Western blot and immunoprecipitation. Samples : A. Modified RIPA extract (10 μg) from the epidermis of FVB wild-type (wt) or galectin 7 transgenic (TG) mice. B. Modified RIPA extract (20 μg) from the epidermis of FVB wild-type mice. Antibody : ab10482 used at 0.005 (A) or 0.0025 (B) μg/ml for WB and as indicated for IP. Detection : Chemiluminescence with 1 second exposure time.

ab10481 detection of mouse galectin 7 by Western blot and immunoprecipitation. Samples : A. Modified RIPA extract (10 µg) from the epidermis of FVB wild-type (wt) or galectin 7 transgenic (TG) mice. B. Modified RIPA extract (20 µg) from the epidermis of FVB wild-type mice. Antibody : ab10482 used at 0.005 (A) or 0.0025 (B) µg/ml for WB and as indicated for

All lanes:

Western blot - Anti-Galectin 7 antibody (ab10482)

Predicted band size: 15 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, ICC/IF, IHC-P, IP

applications

Immunogen

Synthetic Peptide within Human LGALS7 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P47929

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "10-20 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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제품 세부 정보

The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. Differential and in situ hybridizations indicate that this lectin is specifically expressed in keratinocytes. It is expressed at all stages of epidermal differentiation (i.e., in basal and suprabasal layers). The protein was found mainly in stratified squamous epithelium. It is moderately repressed by retinoic acid. The antigen localized to basal keratinocytes, although it was also found at lower levels in the suprabasal layers, where it concentrated to areas of cell-to-cell contact. The cellular localization and its striking down-regulation in cultured keratinocytes imply a role in cell-cell and/or cell-matrix interactions necessary for normal growth control.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

Antibodies were affinity purified using the peptide immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.1% Sodium azide Constituents: PBS, 1.815% Tris, 1.764% Sodium citrate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

+4°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle|Do Not Freeze

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Galectin 7 also known as Gal-7 is a member of the galectin family of beta-galactoside-binding proteins. It has a molecular mass of approximately 15 kDa. Structurally Galectin 7 has a single carbohydrate recognition domain (CRD) which allows it to bind glycoconjugates. Galectin 7 expresses in various tissues with high expression levels in the epidermis and in stratified squamous epithelia.

Biological function summary

Galectin 7 plays a role in cell-cell adhesion apoptosis regulation and immune response modulation. It plays a role in the cell's apoptosis machinery and interacts with the cytochrome c release pathway. This protein does not typically form a part of large complexes functioning individually in most biological processes. It can influence keratinocyte migration indicating involvement in wound healing processes.

Pathways

Galectin 7 participates in the apoptosis and cell adhesion pathways. It works alongside proteins such as Bcl-2 and Bax to regulate programmed cell death by promoting apoptosis. Additionally it interacts with matrix metalloproteinases influencing the remodelling of extracellular matrix a critical component of tissue repair and immune response.

Galectin 7 is associated with cancer and psoriasis. In cancer it can act as a pro-apoptotic factor and modulates tumor progression often in concert with p53 a well-known tumor suppressor protein. In psoriasis aberrant expression of Galectin 7 correlates with impaired epidermal function and inflammation suggesting it may alter the disease course by influencing local immune responses and keratinocyte behavior.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.

See full target information LGALS7

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제품이 사용된 논문 (9)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Frontiers in cellular and infection microbiology 10:583137 PubMed33425778

2020

Autophagy Receptor Tollip Facilitates Bacterial Autophagy by Recruiting Galectin-7 in Response to Group A Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Ching-Yu Lin,Takashi Nozawa,Atsuko Minowa-Nozawa,Hirotaka Toh,Miyako Hikichi,Junpei Iibushi,Ichiro Nakagawa

Clinical and experimental allergy : journal of the 50:922-931 PubMed32474952

2020

Protective role of Galectin-7 for skin barrier impairment in atopic dermatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Takatsune Umayahara,Takatoshi Shimauchi,Manami Iwasaki,Jun-Ichi Sakabe,Masahiro Aoshima,Shinsuke Nakazawa,Tsuyoshi Yatagai,Hayato Yamaguchi,Pawit Phadungsaksawasdi,Kazuo Kurihara,Yoshiki Tokura

Cancers 12: PubMed32290551

2020

High Galectin-7 and Low Galectin-8 Expression and the Combination of both are Negative Prognosticators for Breast Cancer Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Trebo,Nina Ditsch,Christina Kuhn,Helene Hildegard Heidegger,Christine Zeder-Goess,Thomas Kolben,Bastian Czogalla,Elisa Schmoeckel,Sven Mahner,Udo Jeschke,Anna Hester

Cell death and differentiation 26:902-917 PubMed30042493

2018

TRIM17 and TRIM28 antagonistically regulate the ubiquitination and anti-apoptotic activity of BCL2A1.

Applications

Unspecified application

Species

Unspecified reactive species

Loïc Lionnard,Pauline Duc,Margs S Brennan,Andrew J Kueh,Martin Pal,Francesca Guardia,Barbara Mojsa,Maria-Alessandra Damiano,Stéphan Mora,Iréna Lassot,Ramya Ravichandran,Claude Cochet,Abdel Aouacheria,Patrick Ryan Potts,Marco J Herold,Solange Desagher,Jérôme Kucharczak

Scientific reports 7:17086 PubMed29213102

2017

E-cadherin dynamics is regulated by galectin-7 at epithelial cell surface.

Applications

Unspecified application

Species

Unspecified reactive species

Tamara Advedissian,Véronique Proux-Gillardeaux,Rachel Nkosi,Grégoire Peyret,Thao Nguyen,Françoise Poirier,Mireille Viguier,Frédérique Deshayes

Proteome science 13:33 PubMed26719749

2015

Analysis of protein expression in periodontal pocket tissue: a preliminary study.

Applications

Species

Unspecified reactive species

Emanuela Monari,Aurora Cuoghi,Elisa Bellei,Stefania Bergamini,Andrea Lucchi,Aldo Tomasi,Pierpaolo Cortellini,Davide Zaffe,Carlo Bertoldi

PloS one 10:e0119031 PubMed25741714

2015

Overexpression of galectin-7 in mouse epidermis leads to loss of cell junctions and defective skin repair.

Applications

Unspecified application

Species

Unspecified reactive species

Gaëlle Gendronneau,Sadaf Sanii,Tien Dang,Frédérique Deshayes,Delphine Delacour,Evelyne Pichard,Tamara Advedissian,Sukhvinder S Sidhu,Mireille Viguier,Thierry Magnaldo,Francoise Poirier

PloS one 7:e51391 PubMed23236492

2012

Novel potential interacting partners of fibronectin in spontaneous animal model of interstitial cystitis.

Applications

WB, ICC/IF

Species

Cat, Cat

Gudrun Treutlein,Roswitha Dorsch,Kerstin N Euler,Stefanie M Hauck,Barbara Amann,Katrin Hartmann,Cornelia A Deeg

Proteome science 6:18 PubMed18544163

2008

Identification of differentially expressed proteins in spontaneous thymic lymphomas from knockout mice with deletion of p53.

Applications

Species

Unspecified reactive species

Bent Honoré,Søren Buus,Mogens H Claësson

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Galectin 7 antibody

Western blot - Anti-Galectin 7 antibody (AB10482)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Assay Kits

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (9)

Product promise