AB11852

Anti-GATA1 antibody

Name: Anti-GATA1 antibody
Brand: Abcam Limited
SKU: AB11852
Rating: 5 (2 reviews)

(2 리뷰들)

(32 제품이 사용된 논문 )

Anti-GATA1 antibody (ab11852) is a rabbit polyclonal antibody detecting GATA1 in Western Blot. Suitable for Human.

- Over 20 publications
- Trusted since 2004

대체 명칭 보기

ERYF1, GF1, GATA1, Erythroid transcription factor, Eryf1, GATA-binding factor 1, NF-E1 DNA-binding protein, GATA-1, GF-1

1 이미지

Unknown

Western blot - Anti-GATA1 antibody (AB11852)

Lane 1:

Western blot - Anti-GATA1 antibody (ab11852) at 1/400 dilution

Lane 2:

Western blot - Anti-GATA1 antibody (ab11852) at 1/800 dilution

Lane 3:

Negative control

All lanes:

K562 Human erythroleukamia cells

Predicted band size: 43 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

Synthetic Peptide within Human GATA1 aa 350-450 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P15976

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/400 - 1/800", "WB-species-notes": "<p></p>" } } }

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제품 세부 정보

What is this antibody validated in?
Anti-GATA1 antibody (ab11852) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB) in Human samples.

What is the molecular weight of GATA1?
Anti-GATA1 (ab11852) specifically detects a band for GATA1 (UniProt: P15976) at a molecular weight of 43kDa.

Trusted by the scientific community
Anti-GATA1 (ab11852) was first used in a scientific publication in 2004 and has been cited over 20 times in peer-reviewed journals.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.097% Sodium azide Constituents: PBS, 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GATA1 also known as GATA-binding factor 1 is a transcription factor weighing approximately 42 kDa. This protein binds to the DNA sequence "GATA" within the regulatory regions of target genes to control their expression. GATA1 is mostly expressed in hematopoietic cells especially erythroid cells megakaryocytes eosinophils and mast cells. Its specific expression pattern allows it to regulate genes essential for the development and function of these cell types.

Biological function summary

GATA1 regulates gene expression and is essential for erythroid and megakaryocyte differentiation. GATA1 belongs to a family of GATA transcription factors and can form regulatory complexes with other proteins such as FOG1 (Friend of GATA1) and TAL1. These complexes facilitate the binding of GATA1 to DNA enhancing its transcriptional activity on target gene sets necessary for blood cell maturation and function.

Pathways

GATA1 participates in the erythropoiesis and megakaryopoiesis pathways. It directly regulates several genes and coordinates signals with factors like GATA2 and RUNX1 which are other key proteins involved in these pathways. Through these interactions GATA1 ensures the correct development of red blood cells and platelets maintaining hematopoietic homeostasis in the organism.

Abnormal GATA1 function is linked to blood-related disorders. Mutations or dysregulations of GATA1 can lead to diseases such as X-linked thrombocytopenia with dyserythropoietic anemia which involves low platelet count and ineffective red blood cell production. Moreover GATA1 mutations are associated with certain leukemias particularly acute megakaryoblastic leukemia. GATA1's dysfunction in these diseases often occurs in conjunction with abnormalities in related proteins like GATA2 influencing disease progression and phenotype.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Transcriptional activator or repressor which serves as a general switch factor for erythroid development (PubMed : 35030251). It binds to DNA sites with the consensus sequence 5'-[AT]GATA[AG]-3' within regulatory regions of globin genes and of other genes expressed in erythroid cells. Activates the transcription of genes involved in erythroid differentiation of K562 erythroleukemia cells, including HBB, HBG1/2, ALAS2 and HMBS (PubMed : 24245781).

See full target information GATA1

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제품이 사용된 논문 (32)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of clinical investigation 135: PubMed40424086

2025

Splicing of erythroid transcription factor is associated with therapeutic response in myelodysplastic syndromes.

Applications

Unspecified application

Species

Unspecified reactive species

Srinivas Aluri,Te Ling,Ellen Fraint,Samarpana Chakraborty,Kevin Zhang,Aarif Ahsan,Leah Kravets,Gowri Poigaialwar,Rongbao Zhao,Kith Pradhan,Anitria Cotton,Kimo Bachiashvili,Jung-In Yang,Anjali Budhathoki,Beamon Agarwal,Shanisha Gordon Mitchell,Milagros Carbajal,Srabani Sahu,Jacqueline Boultwood,Andrea Pellagatti,Ulrich Steidl,Amittha Wickrema,Satish Nandakumar,Aditi Shastri,Rajasekhar Nvs Suragani,Teresa V Bowman,John D Crispino,Sadanand Vodala,Amit Verma

Journal of sickle cell disease 1:yoae008 PubMed40304012

2025

Hydroxyurea reduces the levels of the fetal gene repressors ZBTB7A/LRF and BCL11A in erythroid cells .

Applications

Unspecified application

Species

Unspecified reactive species

Gabriella E Martyn,Phillip A Doerfler,Yu Yao,Kate G R Quinlan,Mitchell J Weiss,Merlin Crossley

Nucleic acids research 51:6634-6653 PubMed37254808

2023

Multidimensional profiling reveals GATA1-modulated stage-specific chromatin states and functional associations during human erythropoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Dong Li,Xin-Ying Zhao,Shuo Zhou,Qi Hu,Fan Wu,Hsiang-Ying Lee

Scientific reports 12:20587 PubMed36447001

2022

Mechanism of KIT gene regulation by GATA1 lacking the N-terminal domain in Down syndrome-related myeloid disorders.

Applications

Unspecified application

Species

Unspecified reactive species

Rika Kanezaki,Tsutomu Toki,Kiminori Terui,Tomohiko Sato,Akie Kobayashi,Ko Kudo,Takuya Kamio,Shinya Sasaki,Koji Kawaguchi,Kenichiro Watanabe,Etsuro Ito

Nature genetics 54:1417-1426 PubMed35941187

2022

HIC2 controls developmental hemoglobin switching by repressing BCL11A transcription.

Applications

Unspecified application

Species

Unspecified reactive species

Peng Huang,Scott A Peslak,Ren Ren,Eugene Khandros,Kunhua Qin,Cheryl A Keller,Belinda Giardine,Henry W Bell,Xianjiang Lan,Malini Sharma,John R Horton,Osheiza Abdulmalik,Stella T Chou,Junwei Shi,Merlin Crossley,Ross C Hardison,Xiaodong Cheng,Gerd A Blobel

Nature genetics 53:1177-1186 PubMed34341563

2021

Activation of γ-globin gene expression by GATA1 and NF-Y in hereditary persistence of fetal hemoglobin.

Applications

Unspecified application

Species

Unspecified reactive species

Phillip A Doerfler,Ruopeng Feng,Yichao Li,Lance E Palmer,Shaina N Porter,Henry W Bell,Merlin Crossley,Shondra M Pruett-Miller,Yong Cheng,Mitchell J Weiss

Nature communications 12:4439 PubMed34290235

2021

Reactivation of a developmentally silenced embryonic globin gene.

Applications

Unspecified application

Species

Unspecified reactive species

Andrew J King,Duantida Songdej,Damien J Downes,Robert A Beagrie,Siyu Liu,Megan Buckley,Peng Hua,Maria C Suciu,A Marieke Oudelaar,Lars L P Hanssen,Danuta Jeziorska,Nigel Roberts,Stephanie J Carpenter,Helena Francis,Jelena Telenius,Aude-Anais Olijnik,Jacqueline A Sharpe,Jacqueline Sloane-Stanley,Jennifer Eglinton,Mira T Kassouf,Stuart H Orkin,Len A Pennacchio,James O J Davies,Jim R Hughes,Douglas R Higgs,Christian Babbs

Nature communications 12:3806 PubMed34155213

2021

A gain-of-function single nucleotide variant creates a new promoter which acts as an orientation-dependent enhancer-blocker.

Applications

Unspecified application

Species

Unspecified reactive species

Yavor K Bozhilov,Damien J Downes,Jelena Telenius,A Marieke Oudelaar,Emmanuel N Olivier,Joanne C Mountford,Jim R Hughes,Richard J Gibbons,Douglas R Higgs

Cell death & disease 12:223 PubMed33637692

2021

Regulation of MYB by distal enhancer elements in human myeloid leukemia.

Applications

Unspecified application

Species

Unspecified reactive species

Mengjia Li,Penglei Jiang,Kai Cheng,Zehui Zhang,Shuyu Lan,Xiaoxia Li,Lirong Zhao,Yucheng Wang,Xiang Wang,Jing Chen,Tao Ji,Bingshe Han,Junfang Zhang

Blood 137:1327-1339 PubMed33512425

2021

Dynamic CTCF binding directly mediates interactions among cis-regulatory elements essential for hematopoiesis.

Applications

Unspecified application

Species

Unspecified reactive species

Qian Qi,Li Cheng,Xing Tang,Yanghua He,Yichao Li,Tiffany Yee,Dewan Shrestha,Ruopeng Feng,Peng Xu,Xin Zhou,Shondra Pruett-Miller,Ross C Hardison,Mitchell J Weiss,Yong Cheng

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GATA1 antibody

Western blot - Anti-GATA1 antibody (AB11852)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (32)

Product promise