Anti-GATA3 antibody [KT77]

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Immunocytochemistry/ Immunofluorescence - Anti-GATA3 antibody [KT77] (AB110093)

Immunocytochemistry-immunofluorescence using Anti-GATA3 antibody [KT77], ab110093. Publication image from Sage, A. P. et al., 2017, Nat Commun, 28589929. Legend direct from paper.

ILC2 in para-aortic adipose tissue and FALCs of atherosclerotic mice.Flow cytometric analysis (a) and quantification (b,c) of ILC2 present in the para-aortic adipose tissue (AT) show a phenotype similar to KLRG1hi ST2− ILC2 (iILC2-like) in the lymph node in contrast to KLRG1+ ST2+ ILC2 (nILC2-like) prevalent in the peri-gonadal WAT. Mean fluorescence intensity (MFI) for ST2 expression in ILC2 is shown in c. Bars represent mean values. (d) Oil red O and Haematoxylin show that FALCs are present in the para-aortic AT of aged Apoe−/− mice (78–80 weeks of age n=10, scale bar 100 µm) (e) and that Apoe−/− FALCs are greater in both number and size compared to WT (f). Immunoflorescence staining demonstrates that para-aortic FALCs are rich in CD3+ T cells, B220+ B cells and CD138+ plasma cells. CD35+ follicular dendritic cells were absent from these structures (scale bar 50 µm). Additionally, para-aortic FALC-resident CD3− GATA3+ ICOS+ ILC2 cells were also detected (g, scale bar 10 µm). Representative images shown. Graph data points represent individual mice. Statistical significance was determined by Mann–Whitney U-test.