AB139357

Anti-GBAS antibody [OTI1B8]

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Mouse Monoclonal GBAS antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Dog, African green monkey, Recombinant fragment - Human, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human NIPSNAP2.

대체 명칭 보기

GBAS, NIPSNAP2, Protein NipSnap homolog 2, NipSnap2, Glioblastoma-amplified sequence

9 이미지

Immunocytochemistry/ Immunofluorescence - Anti-GBAS antibody [OTI1B8] (AB139357)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GBAS antibody [OTI1B8] (AB139357)

HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for GBAS (green) using ab139357 at 1/100 dilution in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)

Paraffin-embedded human colon adenocarcinoma tissue stained for GBAS using ab139357 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)

Paraffin-embedded human ovary adenocarcinoma tissue stained for GBAS using ab139357 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)

Paraffin-embedded human kidney tissue stained for GBAS using ab139357 at 1/150 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)

Paraffin-embedded human endometrium adenocarcinoma tissue stained for GBAS using ab139357 at 1/150 dilution in immunohistochemical analysis.

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GBAS antibody [OTI1B8] (AB139357)

COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY GBAS stained for GBAS (green) using ab139357 at 1/50 dilution in ICC/IF.

Unknown

Western blot - Anti-GBAS antibody [OTI1B8] (AB139357)

All lanes:

Western blot - Anti-GBAS antibody [OTI1B8] (ab139357) at 1/200 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate transfected with the pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cell lysate transfected with pCMV6-ENTRY GBAS cDNA at 5 µg

Predicted band size: 34 kDa

false

Supplier Data

Western blot - Anti-GBAS antibody [OTI1B8] (AB139357)

All lanes:

Western blot - Anti-GBAS antibody [OTI1B8] (ab139357) at 1/200 dilution

Lane 1:

Human testis extract at 10 µg

Lane 2:

Human uterus extract at 10 µg

Lane 3:

Human breast extract at 10 µg

Lane 4:

Human brain extract at 10 µg

Lane 5:

Human liver extract at 10 µg

Lane 6:

Human ovary extract at 10 µg

Lane 7:

Human thyroid gland extract at 10 µg

Lane 8:

Human colon extract at 10 µg

Predicted band size: 34 kDa

false

Unknown

Western blot - Anti-GBAS antibody [OTI1B8] (AB139357)

All lanes:

Western blot - Anti-GBAS antibody [OTI1B8] (ab139357) at 1/200 dilution

Lane 1:

HepG2 (human liver hepatocellular carcinoma cell line) cell extract at 35 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract at 35 µg

Lane 3:

SVT2 cell extract at 35 µg

Lane 4:

A549 (human lung carcinoma cell line) cell extract at 35 µg

Lane 5:

COS7 (african green monkey kidney fibroblast-like cell line) cell extract at 35 µg

Lane 6:

Jurkat (human T cell leukemia cell line from peripheral blood) cell extract at 35 µg

Lane 7:

MDCK (canine kidney cell line) cell extract at 35 µg

Lane 8:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell extract at 35 µg

Lane 9:

MCF7 (human breast adenocarcinoma cell line) cell extract at 35 µg

Predicted band size: 34 kDa

false

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI1B8

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Dog, Human, African green monkey

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human NIPSNAP2.

O75323

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Dog": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/2000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/100", "ICCIF-species-notes": "" } } }

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제품 세부 정보

Clone OTI1B8 (formerly 1B8).

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification

Purification 관련 사항

Purified from cell culture supernatant by affinity chromatography.

보관 버퍼

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The GBAS protein also known as Golgi apparatus-specific brefeldin A-resistance guanine nucleotide exchange factor 1 (GBF1) is a significant protein in cellular mechanics. GBAS has an approximate molecular weight of 200 kDa. You can find its expression mostly in tissues with high cellular turnover rates such as epithelial tissues and certain types of immune cells. This protein mainly functions within the Golgi apparatus where it plays an important role in regulating vesicular trafficking.

Biological function summary

GBAS contributes to essential cellular processes by participating in the activation of ADP-ribosylation factors (ARFs) as part of a larger protein complex. The protein helps control cargo sorting and lipid composition within the Golgi influencing the secretory pathway. Because of its role in regulating ARFs GBAS is vital in the maintenance of membrane trafficking and cytoskeletal organization impacting cell morphology and function.

Pathways

GBAS is deeply involved in the secretory and endocytic pathways impacting cellular homeostasis. It interacts with proteins like ARF4 and ARF5 ensuring efficient vesicle formation and transport. GBAS acts as a bridge within pathways that manage protein and lipid modifications prior to cell surface delivery or lysosomal degradation.

Research has linked GBAS activity to neurodegenerative disorders and certain cancers given its role in vesicular trafficking. Its dysregulation in neurodegenerative diseases often points to malfunction in ARF-dependent pathways that affect neuron maintenance. In cancers alterations in GBAS function can lead to changes in cellular growth and differentiation frequently involving proteins like ARF1 highlighting its potential as a biomarker or therapeutic target.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Protein involved in mitophagy by facilitating recruitment of the autophagy machinery required for clearance of damaged mitochondria (PubMed : 30982665). Accumulates on the mitochondria surface in response to mitochondrial depolarization and acts as a 'eat me' signal by recruiting proteins involved in selective autophagy, such as autophagy receptors (CALCOCO2/NDP52, NBR1, SQSTM1/p62, TAX1BP1 and WDFY3/ALFY) and ATG8 family proteins (MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAP, GABARAPL1 and GABARAPL2) (PubMed : 30982665).

See full target information NIPSNAP2

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Nature communications 14:5332 PubMed37658083

2023

Atezolizumab plus stereotactic ablative radiotherapy for medically inoperable patients with early-stage non-small cell lung cancer: a multi-institutional phase I trial.

Applications

Unspecified application

Species

Unspecified reactive species

Arta M Monjazeb,Megan E Daly,Guillaume Luxardi,Emanual Maverakis,Alexander A Merleev,Alina I Marusina,Alexander Borowsky,Amin Mirhadi,Stephen L Shiao,Laurel Beckett,Shuai Chen,David Eastham,Tianhong Li,Logan V Vick,Heather M McGee,Frances Lara,Leslie Garcia,Leigh Anne Morris,Robert J Canter,Jonathan W Riess,Kurt A Schalper,William J Murphy,Karen Kelly

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GBAS antibody [OTI1B8]

Immunocytochemistry/ Immunofluorescence - Anti-GBAS antibody [OTI1B8] (AB139357)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GBAS antibody [OTI1B8] (AB139357)

Immunocytochemistry/ Immunofluorescence - Anti-GBAS antibody [OTI1B8] (AB139357)

Western blot - Anti-GBAS antibody [OTI1B8] (AB139357)

Western blot - Anti-GBAS antibody [OTI1B8] (AB139357)

Western blot - Anti-GBAS antibody [OTI1B8] (AB139357)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise