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AB112998

Anti-GCDH/GCD antibody [3E9BA3BF5]

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(1 출판물)

Mouse Monoclonal GCDH/GCD antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human samples. Cited in 1 publication.

대체 명칭 보기

GCD, GCDH

2 이미지
Flow Cytometry - Anti-GCDH/GCD antibody [3E9BA3BF5] (AB112998)
  • Flow Cyt

Unknown

Flow Cytometry - Anti-GCDH/GCD antibody [3E9BA3BF5] (AB112998)

ab112998 at 1ug/ml staining GCDH/GCD in HeLa cells by Flow Cytometry (blue). Isotype control antibody (red).

Immunocytochemistry/ Immunofluorescence - Anti-GCDH/GCD antibody [3E9BA3BF5] (AB112998)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GCDH/GCD antibody [3E9BA3BF5] (AB112998)

ab112998 at 1 μg/ml staining GCDH/GCD in fibroblast cells by immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 594 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (red). Nuclei were stained with DAPI.

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

3E9BA3BF5

Isotype

IgG1

Light chain 타입

kappa

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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대체품

Primary Antibodies

AB182152

Anti-GCDH/GCD antibody [EPR14864(B)]

RabMAb|Recombinant

Flow Cytometry (Intracellular) - Anti-GCDH/GCD antibody [EPR14864(B)] (AB182152)

  • 0
  • 0
Primary Antibodies

AB232774

Anti-GCDH/GCD antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCDH/GCD antibody (AB232774)

  • 0
  • 0

보완 제품

Primary Antibodies

AB110306

Anti-DLST antibody [9F4BD5]

Flow Cytometry - Anti-DLST antibody [9F4BD5] (AB110306)

  • 4
  • 3
Primary Antibodies

AB125066

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066)

  • 5
  • 13
Proteins & Peptides

AB98118

Recombinant Human GCDH/GCD protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human GCDH/GCD protein (His tag N-Terminus) (AB98118)

  • 0
  • 0
Primary Antibodies

AB213511

Anti-mCherry antibody [EPR20579]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Flow Cytometry - Anti-mCherry antibody [EPR20579] (AB213511)

  • 4
  • 6
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg/mL", "FlowCyt-species-notes": "<p>0.1% Triton X-100 permeabilization recommended.</p><p><a href='/ko/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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제품 세부 정보

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Proprietary technique
Purification 관련 사항
ab112998 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%).
보관 버퍼
pH: 7.5 Preservative: 0.02% Sodium azide Constituents: 99.98% HEPES buffered saline
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GCDH also known as glutaryl-CoA dehydrogenase is an enzyme involved in the catabolic breakdown of specific amino acids. This protein harbors a mass of approximately 45 kDa and functions primarily within the mitochondria. GCDH expression occurs in various tissues including the liver kidney and brain. The enzyme plays a mechanical role by catalyzing the decarboxylation of glutaryl-CoA to crotonyl-CoA facilitating the removal of carbon dioxide in this biochemical process.
Biological function summary

This enzyme acts as an important component in the lysine hydroxylysine and tryptophan degradation pathways. GCDH does not form part of a larger enzyme complex but performs its function independently ensuring the proper degradation and metabolism of amino acids. Its activity aids in maintaining metabolic homeostasis preventing the accumulation of harmful intermediates within cells. GCDH may interact with cofactors like flavin adenine dinucleotide (FAD) to carry out its enzymatic activity.

Pathways

GCDH plays a significant role in the lysine and tryptophan metabolism pathways. Within these pathways GCDH helps convert glutaryl-CoA a molecule derived from amino acid catabolism into intermediates ultimately leading to energy production. The enzyme's functionality connects with other mitochondrial enzymes like isovaleryl-CoA dehydrogenase (IVD) which also participates in amino acid breakdown. Proper interaction and coordination within these pathways are essential for maintaining metabolic balance in the cell.

GCDH is closely linked to glutaric aciduria type 1 (GA1) an inborn error of metabolism. This disorder arises from mutations in the GCDH gene leading to insufficient enzyme activity and subsequent buildup of glutaric acid. This accumulation can cause severe neurological damage over time if untreated. GA1 can show genetic connections with other metabolic disorders involving enzymes like medium-chain acyl-CoA dehydrogenase (MCAD). Understanding these interactions helps in diagnosing and managing such metabolic disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Catalyzes the oxidative decarboxylation of glutaryl-CoA to crotonyl-CoA and CO(2) in the degradative pathway of L-lysine, L-hydroxylysine, and L-tryptophan metabolism. It uses electron transfer flavoprotein as its electron acceptor. Isoform Short is inactive.
See full target information GCDH
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death & disease 15:541 PubMed39080260

2024

HMGA1 promotes the progression of esophageal squamous cell carcinoma by elevating TKT-mediated upregulation of pentose phosphate pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Jie Liu,Yuan Zhao,Qiu-Tong Li,Xin-Yuan Lei,Kai-Yue He,Jin-Rong Guo,Jing-Yu Yang,Zhen-Hua Yan,Dan-Hui Wu,Lei Zhang,Yong-Ping Jian,Zhi-Xiang Xu
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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