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AB250117

Anti-GCET1 antibody [EPR12224] - BSA and Azide free

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Rabbit Recombinant Monoclonal GCET1 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 1 publication.
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Western blot - Anti-GCET1 antibody [EPR12224] - BSA and Azide free (AB250117)
  • WB

Supplier Data

Western blot - Anti-GCET1 antibody [EPR12224] - BSA and Azide free (AB250117)

This data was developed using ab179814, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GCET1 antibody [EPR12224] (<a href='/ko/products/primary-antibodies/gcet1-antibody-epr12224-ab179814'>ab179814</a>) at 1/1000 dilution

Lane 1:

MCF7 cell lysate at 10 µg

Lane 2:

Raji cell lysate at 10 µg

Lane 3:

Daudi cell lysate at 10 µg

Predicted band size: 47 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12224

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Sample Prep & Detection Kits

AB201795

Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link®

ELISA - Biotinylation Kit / Biotin Conjugation Kit (Fast, Type A) - Lightning-Link® (AB201795)

  • 0
  • 0
Sample Prep & Detection Kits

AB269903

Gold-Maleimide Conjugation Kit (10nm, 20 OD)

Lateral flow assay - Gold-Maleimide Conjugation Kit (10nm, 20 OD) (AB269903)

  • 0
  • 0
Primary Antibodies

AB3508

Anti-Vitamin D Receptor antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vitamin D Receptor antibody (AB3508)

  • 4
  • 6
Primary Antibodies

AB105227

Anti-ICOS antibody [SP98]

20ul selling size|BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ICOS antibody [SP98] (AB105227)

  • 3
  • 2
Primary Antibodies

AB134055

Anti-FOXP1 antibody [EPR4113]

20ul selling size|RabMAb|Recombinant|Advanced Validation

Flow Cytometry (Intracellular) - Anti-FOXP1 antibody [EPR4113] (AB134055)

  • 0
  • 0
Primary Antibodies

AB75877

Anti-CD5 antibody [EP2952]

RabMAb|Recombinant|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD5 antibody [EP2952] (AB75877)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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제품 세부 정보

ab250117 is the carrier-free version of ab179814.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GCET1 or germinal center B-cell expressed transcript 1 is a protein also known as CLL-1. It weighs approximately 43 kDa. This protein primarily appears in germinal center B-cells and shows up in some cases of diffuse large B-cell lymphoma (DLBCL) and follicular lymphoma. It usually marks the cells involved in proliferation processes which are critical in some lymphomas’ pathogenesis and progression.
Biological function summary

GCET1 participates in the regulation of B-cell proliferation and survival although it is not itself a signaling molecule. It operates mainly within the immune system particularly affecting B-cells. This protein does not typically form part of a larger complex but may influence the activity of other proteins tied to B-cell activation and development aiding in the control of immune responses.

Pathways

GCET1 shows involvement with B-cell receptor signaling and associated signaling pathways. These pathways are central to lymphocyte development activation and differentiation. It interacts closely with essential signaling proteins like SYK and BLNK. Alterations in these pathways can contribute to improper B-cell functioning and linked immunological abnormalities.

GCET1 often relates to lymphoma particularly diffuse large B-cell lymphoma and follicular lymphoma. The protein GCET1 shows reciprocal connections with other molecules like BCL-6 in these cancers. Understanding GCET1 helps in identifying potential therapeutic targets and aids in diagnosing certain lymphoid malignancies underlining its value in clinical research related to these conditions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Protease inhibitor that inhibits trypsin and trypsin-like serine proteases (in vitro). Inhibits plasmin and thrombin with lower efficiency (in vitro).
See full target information SERPINA9

대체 명칭 보기

GCET1, SERPINA11, UNQ692/PRO1337, SERPINA9, Serpin A9, Centerin, Germinal center B-cell-expressed transcript 1 protein
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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of biological chemistry 295:12868-12884 PubMed32580945

2020

Adipose mesenchymal stem cell-derived extracellular vesicles containing microRNA-26a-5p target TLR4 and protect against diabetic nephropathy.

Applications

Unspecified application

Species

Unspecified reactive species

Yurui Duan,Qingyang Luo,Yun Wang,Yali Ma,Fang Chen,Xiaoguang Zhu,Jun Shi
제품이 사용된 논문 모두 보기
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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