Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human skeletal muscl tissue labeling GCH1 with ab307507 at 1/500 (1.044 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : No staining on human skeletal muscle.The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling GCH1 with ab307507 at 1/500 (1.044 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human pancreatic islets.The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling GCH1 with ab307507 at 1/500 (1.044 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on scattered cells in human colon (PMID : 22753274). The section was incubated with ab307507 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse midbrain tissue labeling GCH1 with ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. Positive staining on neurons of mouse midbrain (PMID : 10907721). The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse stomach tissue labeling GCH1 with ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. Positive staining on scattered cells in mouse stomach.The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscl tissue labeling GCH1 with ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. Negative control : No staining on mouse skeletal muscle.The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling GCH1 with ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Negative control : No staining on rat skeletal muscle.The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat pancreas tissue labeling GCH1 with ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on rat pancreatic islets.The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling GCH1 with ab307507 at 1/1000 (0.522 ug/ml) followed by a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. Positive staining on scattered cells in rat colon.The section was incubated with ab307507 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND^® RX instrument Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond^® Polymer Refine Detection) was used. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IP

Supplier Data

Immunoprecipitation - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using ab307507, the same antibody clone in a different buffer formulation. GCH1 was immunoprecipitated from 0.35 mg Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug with ab307507 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab307507 at 1/1000 dilution. VeriBlot for IP secondary antibody(HRP)(ab131366) was used at 1/5000 dilution. Lane 1 : Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 ug Lane 2 : ab307507 IP in Neuro-2a whole cell lysate Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab307507 in Neuro-2a whole cell lysate Blocking and dilution buffer and concentration : 5% NFDM/TBST. Exposure time : 6 seconds

All lanes:

Immunoprecipitation - Anti-GCH1 antibody [EPR27331-30] (<a href='/ko/products/primary-antibodies/gch1-antibody-epr27331-30-ab307507'>ab307507</a>) at 1/1000 dilution

Lane 1:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 10 μg

Lane 2:

Neuro-2a whole cell lysate

Lane 3:

Rabbit monoclonal IgG (<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a>) instead of <a href='/ko/products/primary-antibodies/gch1-antibody-epr27331-30-ab307507'>ab307507</a> in Neuro-2a whole cell lysate

Secondary

All lanes:

Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/5000 dilution

Observed band size: 27 kDa

false

Exposure time: 6s

• WB

Supplier Data

Western blot - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using 307507, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Negative control : K562 (PMID : 32778843) Exposure time :

All lanes:

Western blot - Anti-GCH1 antibody [EPR27331-30] (<a href='/ko/products/primary-antibodies/gch1-antibody-epr27331-30-ab307507'>ab307507</a>) at 1/1000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma epithelial cell) whole cell lysate 20 μg

Lane 2:

K562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 20 μg

Lane 3:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate 20 μg

Lane 4:

N9 (mouse microglia) whole cell lysate 20 μg

Lane 5:

PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 27 kDa

false

Exposure time: 37s

• WB

Supplier Data

Western blot - Anti-GCH1 antibody [EPR27331-30] - BSA and Azide free (AB307508)

This data was developed using 307507, the same antibody clone in a different buffer formulation. Blocking and diluting buffer and concentration : Negative control : skeletal muscle (PMID : 22798524) 180 seconds Exposure time :

All lanes:

Western blot - Anti-GCH1 antibody [EPR27331-30] (<a href='/ko/products/primary-antibodies/gch1-antibody-epr27331-30-ab307507'>ab307507</a>) at 1/1000 dilution

Lane 1:

Mouse liver tissue lysate 20 μg

Lane 2:

Mouse hypothalamus tissue lysate 20 μg

Lane 3:

Mouse skeletal muscle tissue lysate 20 μg

Lane 4:

Rat liver tissue lysate 20 μg

Lane 5:

Rat hypothalamus tissue lysate 20 μg

Lane 6:

Rat skeletal muscle tissue lysate 20 μg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 27 kDa

false

Exposure time: 180s