Anti-Geminin antibody [1A8]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [1A8] (AB104306)

ab104306, at 3 μg/ml, staining Geminin in formalin-fixed paraffin-embedded Human lymph node by Immunohistochemistry.

This image was generated using the ascites version of the product.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Geminin antibody [1A8] (AB104306)

ab104306, at 1μg/ml, staining Geminin in formalin-fixed paraffin-embedded Human placenta by Immunohistochemistry.

This image was generated using the ascites version of the product.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [1A8] (AB104306)

ab104306, at 10 μg/ml, staining Geminin in HeLa cells by Immunofluorescence.

This image was generated using the ascites version of the product.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Geminin antibody [1A8] (AB104306)

Immunofluorescence analysis of Caki-2 (Clear cell renal carcinoma) cells, staining Geminin with ab104306.

Cells were fixed with formaldehyde, permeabilized with 0.25% Triton X-100 and blocked with 1% BSA for 30 minutes at 25^oC. Primary antibody was concentrated using ab102778 Antibody Concentration Kit and conjugated to Rhodamine using ab102915 EasyLink Rhodamine Conjugation Kit. Samples were incubated with conjugated primary antibody (20 μg/ml in diluent) for 2 hours at 25^oC.

This image was generated using the ascites version of the product.

This image is courtesy of an Abreview submitted by Melody Jimenez

• Flow Cyt

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Flow Cytometry - Anti-Geminin antibody [1A8] (AB104306)

Overlay histogram showing HeLa cells stained with ab104306 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab104306, 1μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

This image was generated using the ascites version of the product.

• WB

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Western blot - Anti-Geminin antibody [1A8] (AB104306)

The tagged immunogen has a predicted MWt of 37 kDa.

This image was generated using the ascites version of the product.

All lanes:

Western blot - Anti-Geminin antibody [1A8] (ab104306) at 5 µg/mL

All lanes:

Immunogen at 0.2 µg

Predicted band size: 24 kDa

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