Anti-GHRHR antibody

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GHRHR antibody (AB76263)

Immunofluorescence analysis of HUVEC cells using ab76263 at a 1/500 dilution.
Left image un-treated.
Right image treated with immunizing peptide.

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-GHRHR antibody (AB76263)

Immunofluorescence analysis of mouse pituitary cells, staining GHRHR with ab76263.

Cells were fixed with 4% paraformaldehyde and blocked using 4% NGS in PBS with 0.3% Triton X-100 for 1 hour at room temperature. Cells were incubated with primary antibody (1/50) in 4% NGS for 18 hours at 4°C. Slides were washed three times for 15 min with PBS and then incubated in 4% NGS with an AlexaFluor®488-conjugated anti- rabbit secondary antibody (1/1000) for 1 hour at room temperature.

Image from Vakili H et al., Molecular and Cellular Endocrinology: Volume 345, Issues 1–2, 15 October 2011, Pages 48–57. Fig 2.; doi: 10.1016/j.mce.2011.07.010. Epub 2011 Jul 12.

• WB

Unknown

Western blot - Anti-GHRHR antibody (AB76263)

All lanes:

Western blot - Anti-GHRHR antibody (ab76263) at 1/500 dilution

Lane 1:

Extracts from LOVO cells at 5 µg

Lane 2:

Extracts from LOVO cells at 5 µg with immunizing peptide

Predicted band size: 47 kDa

Observed band size: 47 kDa,>117 kDa

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