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AB245613

Anti-Giantin antibody

4

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(0 출판물)

Rabbit Polyclonal Giantin antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human GOLGB1 aa 950 to C-terminus.

대체 명칭 보기

Golgin subfamily B member 1, 372 kDa Golgi complex-associated protein, Giantin, Macrogolgin, GCP372, GOLGB1

2 이미지
Immunoprecipitation - Anti-Giantin antibody (AB245613)
  • IP

Supplier Data

Immunoprecipitation - Anti-Giantin antibody (AB245613)

Giantin was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) using ab245613 at 6 μg/mg lysate. Western blot was performed on the immunoprecipitate using ab245613 at 1 μg/ml.

Lane 1 : ab245613 IP in HEK-293T whole cell lysate.
Lane 2 : Control IgG in HEK-293T whole cell lysate.

Detection : Chemiluminescence with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-Giantin antibody (ab245613)

Predicted band size: 376 kDa

false

Western blot - Anti-Giantin antibody (AB245613)
  • WB

Supplier Data

Western blot - Anti-Giantin antibody (AB245613)

All lanes:

Western blot - Anti-Giantin antibody (ab245613) at 0.1 µg/mL

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 376 kDa

true

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human GOLGB1 aa 950 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q14789

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대체품

Primary Antibodies

AB37266

Anti-Giantin antibody [9B6] - Golgi Marker

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (AB37266)

  • 4
  • 15
Primary Antibodies

AB80864

Anti-Giantin antibody - Golgi Marker

BOND RX™ Validated|KO Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody - Golgi Marker (AB80864)

  • 4
  • 2
Primary Antibodies

AB174655

Anti-Giantin antibody - Golgi Marker

Immunoprecipitation - Anti-Giantin antibody - Golgi Marker (AB174655)

  • 4
  • 3

보완 제품

Primary Antibodies

AB52649

Anti-GM130 antibody [EP892Y] - cis-Golgi Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunoprecipitation - Anti-GM130 antibody [EP892Y] - cis-Golgi Marker (AB52649)

  • 5
  • 39
Primary Antibodies

AB37266

Anti-Giantin antibody [9B6] - Golgi Marker

Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-Giantin antibody [9B6] - Golgi Marker (AB37266)

  • 4
  • 15
Primary Antibodies

AB22595

Anti-Calnexin antibody - ER Marker

Lab Essentials|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-Calnexin antibody - ER Marker (AB22595)

  • 5
  • 28
Primary Antibodies

AB174280

Anti-TGN46 antibody [EPR12676] - Golgi Marker

20ul selling size|RabMAb|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TGN46 antibody [EPR12676] - Golgi Marker (AB174280)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
Purification 관련 사항
ab245613 was affinity purified using an epitope specific to Giantin immobilized on solid support.
보관 버퍼
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Giantin also known as Giantin Golgi or Giantin protein is a large coiled-coil protein with a molecular mass of approximately 376 kDa. It localizes primarily to the cis/medial region of the Golgi apparatus serving as a Golgi marker. As a part of the structural framework of the Golgi it plays an important role in maintaining the integrity and architecture of the Golgi stacks. Giantin is highly expressed in a variety of cell types including those in the liver kidney and brain making it an important target in cell biology studies.
Biological function summary

The Giantin protein functions as a scaffold that supports the Golgi apparatus’s dynamic organization. It is part of a larger complex that influences vesicular trafficking within the cell. This ability to interact with various vesicle coat proteins points to its important role in the sorting and transport of proteins and lipids through the secretory pathway. Besides Giantin is involved in the assembly of the Golgi matrix and influences its disassembly during mitosis suggesting a significant involvement in cell proliferation and division.

Pathways

Giantin helps regulate intracellular transport pathways connecting the endoplasmic reticulum (ER) and Golgi as well as intra-Golgi trafficking. It interfaces with other significant proteins such as GM130 and GRASP65 which are involved in Golgi dynamics and structure. This protein also participates in the coordination of the secretory pathway important for the modification and sorting of proteins destined for the cell surface or lysosomes.

Giantin has been linked to conditions involving impaired protein glycosylation and Golgi-related transport defects. Notably defects in Giantin function are associated with connective tissue disorders and certain forms of Golgi dysfunctions like Congenital Disorders of Glycosylation (CDG). Additionally Giantin’s interactions with proteins such as Sec23/24 and Bet3 which are cargo-binding components of the COPII vesicle coat connect its functional disruption to diseases characterized by impaired vesicular trafficking and cellular secretion malfunctions.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

May participate in forming intercisternal cross-bridges of the Golgi complex.
See full target information GOLGB1
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Product promise

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