Anti-GIP antibody [EPR20410]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] (AB209792)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling GIP with ab209792 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Cytoplasmic staining on alpha cells of human pancreas islet is observed (PMID : 4574902).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] (AB209792)

Immunohistochemical analysis of paraffin-embedded human duodenum tissue labeling GIP with ab209792 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Sporadic and cytoplasmic staining on human duodenum is observed (PMID : 4574902).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-GIP antibody [EPR20410] (AB209792)

Fluorescence multiplex immunohistochemical analysis of the human pancreas (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-Carboxypeptidase A (ab278044, gray; Opal™690), anti-GIP (ab271989, green; Opal™520) and anti-Somatostatin 28 (ab111912, red; Opal™570) on human pancreas. Panel B : anti-Carboxypeptidase A stained on acinar cells. Panel C : anti-GIP stained on alpha cells. Panel D : anti-Somatostatin 28 stained on delta cells. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab278044 at 1/4000 dilution (0.135 μg/ml), ab271989 at 1/4000 dilution (0.27 μg/ml) and ab111912 at 1/9000 dilution (0.068 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. DAPI (blue) was used as a nuclear counter stain. This data was developed using ab271989, the same antibody clone in a different buffer formulation.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-GIP antibody [EPR20410] (AB209792)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded human duodenum tissue staining GIP with ab328032 at a 1 : 2000 (0.239 μg/ml) dilution, ab209792 anti-GIP used at 1 : 4000 (0.258 μg/ml) dilution and ab325645 anti-Peptide YY used at a 1 : 2000 (0.262 μg/ml) dilution.

Panel A : anti-GIP(active) (green; Opal™520), anti-GIP (magenta; Opal™690) and anti-Peptide YY (yellow; Opal™570) on human duodenum.
Panel B : anti-GIP(active) staining enteroendocrine K cells in human duodenum.
Panel C : anti-GIP staining enteroendocrine K cells in human duodenum.
Panel D : anti-Peptide YY staining enteroendocrine cells in human duodenum.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab328032, ab209792 and ab325645 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] (AB209792)

Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling GIP with ab209792 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Cytoplasmic staining on alpha cells of mouse pancreas islet is observed (PMID : 4574902).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-GIP antibody [EPR20410] (AB209792)

Immunohistochemical analysis of 4% paraformaldehyde-fixed 0.2% Triton X-100 permeabilized frozen mouse pancreas tissue labeling GIP with ab209792 at 1/100 dilution followed by Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Cytoplasmic staining on alpha cells of mouse pancreas islet is observed.

The nuclear counterstain is DAPI (blue).

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) at 1/1000 dilution.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] (AB209792)

Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue labeling GIP with ab209792 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Sporadic and cytoplasmic staining on mouse small intestine is observed (PMID : 4574902).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GIP antibody [EPR20410] (AB209792)

Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling GIP with ab209792 at 1/2000 dilution followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Cytoplasmic staining on alpha cells of rat pancreas islet is observed (PMID : 4574902).

Counter stained with Hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-GIP antibody [EPR20410] (AB209792)

Fluorescence multiplex immunohistochemical analysis of the rat pancreas (Formalin/PFA-fixed paraffin-embedded sections).

Panel A : merged staining of anti-Somatostatin 28 (yellow; Opal™520), anti-GIP (magenta; Opal™570) and anti-Pancreatic Polypeptide (green; Opal™690) on rat pancreas.
Panel B : anti-Somatostatin 28 staining the delta cells in rat pancreas islet.
Panel C : anti-GIP staining the alpha cells in rat pancreas islet.
Panel D : anti-Pancreatic Polypeptide staining the PP cells in rat pancreas islet.

Nuclear DNA was labelled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab111912 at 1/9000 dilution (0.07 μg/ml), ab271989 at 1/4000 dilution (0.25 μg/ml) and ab255827 at 1/10000 dilution (0.05 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using ab271989, the same antibody clone in a different buffer formulation.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-GIP antibody [EPR20410] (AB209792)

Fluorescence multiplex immunohistochemical analysis of the rat pancreas (Formalin/PFA-fixed paraffin-embedded sections).

Panel A : merged staining of anti-SV2A (yellow; Opal™520), anti-GIP (magenta; Opal™570) and anti-Pancreatic Polypeptide (green; Opal™690) on rat pancreas.
Panel B : anti-SV2A staining predominantly the beta cells in mouse pancreas islet.
Panel C : anti-GIP staining the alpha cells in rat pancreas islet.
Panel D : anti-Pancreatic Polypeptide staining the PP cells in rat pancreas islet.

Nuclear DNA was labelled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab254351 at 1/1000 dilution (0.48 μg/ml), ab271989 at 1/4000 dilution (0.25 μg/ml) and ab255827 at 1/10000 dilution (0.05 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using ab271989, the same antibody clone in a different buffer formulation.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-GIP antibody [EPR20410] (AB209792)

• mIHC

Lab

Multiplex immunohistochemistry - Anti-GIP antibody [EPR20410] (AB209792)

Fluorescence multiplex immunohistochemical analysis of the mouse pancreas (Formalin/PFA-fixed paraffin-embedded sections).

Panel A : merged staining of anti-Somatostatin 28 (yellow; Opal™520), anti-GIP (magenta; Opal™570) and anti-Pancreatic Polypeptide (green; Opal™690) on mouse pancreas.
Panel B : anti-Somatostatin 28 staining the delta cells in mouse pancreas islet.
Panel C : anti-GIP staining the alpha cells in mouse pancreas islet.
Panel D : anti-Pancreatic Polypeptide staining the PP cells in mouse pancreas islet.

Nuclear DNA was labelled with DAPI (shown in blue). The section was incubated in three rounds of staining : in the order of ab111912 at 1/9000 dilution (0.07 μg/ml), ab271989 at 1/4000 dilution (0.25 μg/ml) and ab255827 at 1/10000 dilution (0.05 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using ab271989, the same antibody clone in a different buffer formulation.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-GIP antibody [EPR20410] (AB209792)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse pancreas tissue staining MafB with ab326360 at a 1 : 100 (5.16 μg/ml) dilution, ab209792 anti-GIP used at 1 : 2000 (0.258 μg/ml) dilution and ab181547 anti-Insulin used at a 1 : 20000 (0.053 μg/ml) dilution.

Panel A : anti-MAFB (green; Opal™570), anti-GIP (magenta; Opal™690) and anti-Insulin (gray; Opal™520) on mouse pancreas.
Panel B : anti-MAFB staining nucleus of alpha cells in mouse pancreas.
Panel C : anti-GIP staining cytoplasm of alpha cells in mouse pancreas.
Panel D : anti-Insulin staining cytoplasm of beta cells in mouse pancreas.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab326360, ab209792 and ab181547 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Nuclear counter stain with DAPI.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-GIP antibody [EPR20410] (AB209792)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded rat pancreas tissue staining MafA with ab324869 at a 1 : 100 (5.13 ug/ml) dilution, ab181547 anti-Insulin used at 1 : 20000 (0.053 ug/ml) dilution and ab209792 anti-GIP used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MafA (green; Opal™520), anti-Insulin (magenta; Opal™690) and anti-GIP (gray; Opal™570) on rat pancreas.
Panel B : anti-MafA staining nucleus of beta cells in rat pancreas islet.
Panel C : anti-Insulin staining cytoplasm of beta cells in rat pancreas islet.
Panel D : anti-GIP staining the alpha cells in rat pancreas islet.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324869, ab181547 and ab209792 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

• mIHC

Supplier Data

Multiplex immunohistochemistry - Anti-GIP antibody [EPR20410] (AB209792)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse pancreas tissue staining MafA with ab324869 at a 1 : 100 (5.13 ug/ml) dilution, ab181547 anti-Insulin used at 1 : 20000 (0.053 ug/ml) dilution and ab209792 anti-GIP used at a 1 : 2000 (0.26 ug/ml) dilution.

Panel A : merged staining of anti-MafA (green; Opal™520), anti-Insulin (magenta; Opal™690) and anti-GIP (gray; Opal™570) on mouse pancreas.
Panel B : anti-MafA staining nucleus of beta cells in mouse pancreas islet.
Panel C : anti-Insulin staining cytoplasm of beta cells in mouse pancreas islet.
Panel D : anti-GIP staining the alpha cells in mouse pancreas islet.
Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324869, ab181547 and ab209792 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.