AB169779

Anti-Glucose Transporter 8 antibody [EPR9477]

RabMAb
Recombinant
각 태그의 뜻

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(4 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal Glucose Transporter 8 antibody. Suitable for WB and reacts with Mouse, Human, Rat samples. Cited in 4 publications.

대체 명칭 보기

GLUT8, GLUTX1, SLC2A8, Glucose transporter type 8, Glucose transporter type X1, GLUT-8

3 이미지

Unknown

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (AB169779)

All lanes:

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (ab169779) at 1/1000 dilution

Lane 1:

Human cerebellum lysate at 10 µg

Lane 2:

Human fetal heart lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 51 kDa

false

Lab

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (AB169779)

Blocking/Diluting Buffer and concentration : 5% NFDM/TBST
The observed bands are consistent with what are described in PMID : 10860996

All lanes:

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (ab169779) at 1/1000 dilution

All lanes:

Human skeletal muscle lysates at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 51 kDa

Observed band size: 38 kDa

false

Lab

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (AB169779)

Blocking/Diluting Buffer and concentration : 5% NFDM/TBST

The observed bands are consistent with what are described in PMID : 10860996

All lanes:

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (ab169779) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 15 µg

Lane 2:

Rat brain tissue lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 51 kDa

Observed band size: 38 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR9477

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "There are publications supporting the capability of GLUT8 to migrate at 35 kDa. See PMID 10671487 and 10860996. Thus, full length GLUT8 may migrate between 35-51 kDa." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "There are publications supporting the capability of GLUT8 to migrate at 35 kDa. See PMID 10671487 and 10860996. Thus, full length GLUT8 may migrate between 35-51 kDa." }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "There are publications supporting the capability of GLUT8 to migrate at 35 kDa. See PMID 10671487 and 10860996. Thus, full length GLUT8 may migrate between 35-51 kDa." } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Glucose Transporter 8 also known as GLUT8 is a member of the sugar transporter family. This protein plays a mechanical role in transferring glucose across cellular membranes. GLUT8 operates with facilitative transport ensuring glucose entry into cells. It has a molecular mass of around 55 kDa. Expression sites for GLUT8 include tissues like the testis brain and placenta. This specific transporter assists in maintaining glucose homeostasis critical for cell metabolism and energy production.

Biological function summary

GLUT8 facilitates the uptake of glucose into cells supporting cellular survival and energetic demands. It does not appear to be part of any large protein complex but operates independently to bring glucose into the intracellular environment. This functionality underpins its involvement in energy regulation influencing cellular respiration and growth by ensuring glucose availability especially in specialized tissues such as the brain and placenta.

Pathways

GLUT8 acts within the insulin signaling and glucose transport pathways. The transporter integrates with the insulin-responsive network where it responds to insulin stimulation to increase glucose uptake in cells expressing it similar to insulin-regulated transporters like GLUT4. GLUT8's functionality ensures effective glucose utilization directly linking its transport activity with energy metabolism pathways.

GLUT8's role connects to metabolic dysfunction and neurological conditions. Research shows a connection to diabetes primarily through its responsive nature to insulin which affects glucose homeostasis. Additionally GLUT8 is linked to Alzheimer's disease with potential interactions with amyloid precursor proteins and insulin signaling disruptions. These connections highlight GLUT8's relevance to neurological and energy balance disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Insulin-regulated facilitative hexose transporter that mediates the transport of glucose and fructose (By similarity). Facilitates hepatic influx of dietary trehalose, which in turn inhibits glucose and fructose influx triggering a starvation signal and hepatic autophagy through activation of AMPK and ULK1 (PubMed : 27922102). Also able to mediate the transport of dehydroascorbate.

See full target information SLC2A8

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

The Journal of neuroscience : the official journal 38:9579-9599 PubMed30232223

2018

Neural Deletion of Glucose Transporter Isoform 3 Creates Distinct Postnatal and Adult Neurobehavioral Phenotypes.

Applications

Unspecified application

Species

Unspecified reactive species

Bo-Chul Shin,Carlos Cepeda,Ana María Estrada-Sánchez,Michael S Levine,Laya Hodaei,Yun Dai,Jai Jung,Amit Ganguly,Peter Clark,Sherin U Devaskar

PloS one 13:e0193583 PubMed29590129

2018

Humanin (HN) and glucose transporter 8 (GLUT8) in pregnancies complicated by intrauterine growth restriction.

Applications

Unspecified application

Species

Unspecified reactive species

Carla Janzen,Margarida Y Y Lei,Il Seok D Jeong,Amit Ganguly,Peggy Sullivan,Vladislava Paharkova,Gina Capodanno,Hiromi Nakamura,Alix Perry,Bo-Chul Shin,Kuk-Wha Lee,Sherin U Devaskar

Nutrition, metabolism, and cardiovascular diseases 26:614-622 PubMed27052924

2016

Impaired stimulation of glucose transport in cardiac myocytes exposed to very low-density lipoproteins.

Applications

Unspecified application

Species

Unspecified reactive species

I Papageorgiou,C Viglino,M-C Brulhart-Meynet,R W James,R Lerch,C Montessuit

Antioxidants & redox signaling 22:848-70 PubMed25602171

2015

Reactive oxygen species-induced TXNIP drives fructose-mediated hepatic inflammation and lipid accumulation through NLRP3 inflammasome activation.

Applications

Unspecified application

Species

Unspecified reactive species

Xian Zhang,Jian-Hua Zhang,Xu-Yang Chen,Qing-Hua Hu,Ming-Xing Wang,Rui Jin,Qing-Yu Zhang,Wei Wang,Rong Wang,Lin-Lin Kang,Jin-Sheng Li,Meng Li,Ying Pan,Jun-Jian Huang,Ling-Dong Kong

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glucose Transporter 8 antibody [EPR9477]

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (AB169779)

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (AB169779)

Western blot - Anti-Glucose Transporter 8 antibody [EPR9477] (AB169779)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (4)

Product promise