Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free (AB232430)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab40845).

All lanes:

Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free (ab232430) at 1/5000 dilution

All lanes:

Human brain lysate at 10 µg

Predicted band size: 101 kDa

Observed band size: 95 kDa

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• WB

Lab

Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] - BSA and Azide free (AB232430)

This data was developed using ab40845, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

In Western blot, Anti-Myc tag antibody [9E10] - Loading Control (ab32) staining at 1/10000 dilution.

This antibody does not cross-react with Human GRIA1,GRIA2 and GRIA4.

Exposure time : ab40845 : 40s, ab32 : 80s.

All lanes:

Western blot - Anti-Glutamate receptor 3/GluA3 antibody [EP813Y] (<a href='/ko/products/primary-antibodies/glutamate-receptor-3-glua3-antibody-ep813y-ab40845'>ab40845</a>) at 1/1000 dilution

Lane 1:

293T transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 20 µg

Lane 2:

293T transfected with Human fragment GRIA2 expression vector containing a c-term myc-His-tag®, whole cell lysate at 20 µg

Lane 3:

293T transfected with Human full length GRIA3 expression vector containing a n-term myc-His-tag®, whole cell lysate at 20 µg

Lane 4:

293T transfected with human full length GRIA4 expression vector containing a c-term myc-His-tag®, whole cell lysate at 20 µg

Lane 5:

Recombinant Human full length GRIA1 Protein containing a C-Myc/DDK tag at 100 ng

Secondary

Lanes 1 - 5:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Lanes 1 - 5:

Goat anti-mouse IgG (H+L), Peroxidase conjugated at 1/10000 dilution

Observed band size: 100 kDa

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