JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB289369

Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free

Be the first to review this product! 리뷰 제출

|

(0 출판물)

Rabbit Recombinant Monoclonal Glutathione Peroxidase 2/GPX2 antibody. Carrier free. Suitable for WB, IHC-P and reacts with Mouse, Rat, Human, Dog samples. Immunogen corresponding to Synthetic Peptide within Human Glutathione peroxidase 2.
3 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)

GPX2 antibody [HL125] detects GPX2 protein at cytoplasm by immunohistochemical analysis.
Sample : Paraffin-embedded mouse colon.
GPX2 stained by GPX2 antibody [HL125] (ab289369) diluted at 1 : 200.
Antigen Retrieval : Citrate buffer, pH 6.0, 15 min

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)

Paraffin-embedded rat tissue stained for Glutathione Peroxidase 2/GPX2 protein with ab289369 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min .

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 2/GPX2 antibody [HL125] - BSA and Azide free (AB289369)

Paraffin-embedded dog kidney tissue stained for Glutathione Peroxidase 2/GPX2 protein at cytoplasm with ab289369 at a 1/100 dilution in immunohistochemical analysis.

Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL125

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Human, Mouse, Dog

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human Glutathione peroxidase 2. The exact immunogen used to generate this antibody is proprietary information.

P18283

style
left-column

보완 제품

Assay Kits

AB102530

Glutathione Peroxidase Assay Kit (Colorimetric)

Functional Studies - Glutathione Peroxidase Assay Kit (Colorimetric) (AB102530)

  • 5
  • 2
Sample Prep & Detection Kits

AB269823

Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link®

Immunocytochemistry - Alexa Fluor® 647 Conjugation Kit (Fast) - Lightning-Link® (AB269823)

  • 5
  • 2
Sample Prep & Detection Kits

AB218260

Oligonucleotide Conjugation Kit

Conjugation - Oligonucleotide Conjugation Kit (AB218260)

  • 0
  • 0
Primary Antibodies

AB22604

Anti-Glutathione Peroxidase 1 antibody

KO Validated

Western blot - Anti-Glutathione Peroxidase 1 antibody (AB22604)

  • 5
  • 23
Primary Antibodies

AB125066

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066)

  • 5
  • 13
Primary Antibodies

AB183664

Anti-GPX8 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPX8 antibody (AB183664)

  • 0
  • 0
Primary Antibodies

AB124954

Anti-TXNRD1 antibody [EPNCIR129]

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-TXNRD1 antibody [EPNCIR129] (AB124954)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "500-3000 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" }, "Dog": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200", "IHCP-species-notes": "<p></p>" } } }
style
left-column

제품 세부 정보

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Glutathione Peroxidase 2 (GPX2) also known as GPX-GI or GPX2 weight belongs to the glutathione peroxidase family. This enzyme plays a mechanical role in reducing hydrogen peroxide by using glutathione which protects cells from oxidative damage. GPX2 is a selenoprotein with a molecular weight of approximately 22 kDa. It is expressed in the gastrointestinal tract particularly in the epithelial cells of the intestines and is also present in other tissues to a lesser extent.
Biological function summary

GPX2 functions as an antioxidant defense mechanism. It works in safeguarding cells by reducing reactive oxygen species and maintaining cellular redox balance. GPX2 is not part of a larger multiprotein complex but works closely with other antioxidant proteins to protect cells. Its expression helps regulate cellular homeostasis and supports tissue integrity especially under oxidative stress conditions.

Pathways

GPX2 continues its relevance in the glutathione metabolism pathway where it cooperates with other glutathione peroxidases and detoxifies peroxides. GPX2 also participates in the oxidative stress response pathway and interacts with proteins like GSSG and GPX1 enhancing the cellular antioxidant capacity. Its activity in these pathways underlines its significant role in maintaining cellular health and integrity by preventing oxidative damage.

GPX2 relates it to colorectal cancer and inflammatory bowel disease. In such conditions GPX2's expression levels are often altered indicating its role in cancer progression and inflammation management. The protein connects to other antioxidant enzymes such as GPX1 and SOD in the context of these diseases with changes in its function potentially impacting disease development and progression.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Catalyzes the reduction of hydroperoxides in a glutathione-dependent manner thus regulating cellular redox homeostasis (PubMed : 36608588, PubMed : 8428933). Can reduce small soluble hydroperoxides such as H2O2, cumene hydroperoxide and tert-butyl hydroperoxide, as well as several fatty acid-derived hydroperoxides (PubMed : 36608588, PubMed : 8428933). Cannot reduce phosphatidycholine hydroperoxide (PubMed : 8428933).
See full target information Glutathione peroxidase 2

대체 명칭 보기

Glutathione peroxidase 2, GPx-2, GSHPx-2, Gastrointestinal glutathione peroxidase, Glutathione peroxidase-gastrointestinal, Glutathione peroxidase-related protein 2, Phospholipid hydroperoxide glutathione peroxidase GPX2, GPx-GI, GSHPx-GI, GPRP-2, GPX2
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com