Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free

• ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (AB244234)

This data was developed using ab128933, the same antibody clone in a different buffer formulation.ab128933, at a 1/100 dilution, staining Human Glutathione Reductase in HeLa cells, by Immunofluorescence.

• Flow Cyt (Intra)

Lab

Flow Cytometry (Intracellular) - Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (AB244234)

This data was developed using ab128933, the same antibody clone in a different buffer formulation.
Flow Cytometry analysis of HeLa (Human cervix adenocarcinoma epithelial cell) cells labeling Glutathione Reductase with purified ab128933 at 1/100 dilution (10 µg/mL) (Red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cells without incubation with primary antibody and secondary antibody (Blue).

• WB

Lab

Western blot - Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (AB244234)

This data was developed using ab128933, the same antibody clone in a different buffer formulation.

Lane 1 : Wild-type HAP1 cell lysate (20 μg)

Lane 2 : Glutathione Reductase knockout HAP1 cell lysate (20 μg)

Lane 3 : HeLa cell lysate (20 μg)

Lane 4 : Jurkat cell lysate (20 μg)

Lanes 1 - 4 : Merged signal (red and green). Green - ab128933 observed at 55 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab128933 was shown to specifically react with Glutathione Reductase when Glutathione Reductase knockout samples were used, along with additional cross-reactive bands. Wild-type and Glutathione Reductase knockout samples were subjected to SDS-PAGE. ab128933 and ab8245 (loading control to GAPDH) were diluted at 1/10000 and 1/10,000 dilution respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-Glutathione Reductase antibody [EPR7237] (<a href='/ko/products/primary-antibodies/glutathione-reductase-antibody-epr7237-ab128933'>ab128933</a>)

Predicted band size: 56 kDa

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• WB

Unknown

Western blot - Anti-Glutathione Reductase antibody [EPR7237] - BSA and Azide free (AB244234)

This data was developed using ab128933, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Glutathione Reductase antibody [EPR7237] (<a href='/ko/products/primary-antibodies/glutathione-reductase-antibody-epr7237-ab128933'>ab128933</a>) at 1/10000 dilution

Lane 1:

Caco-2 cell lysate at 10 µg

Lane 2:

A549 cell lysate at 10 µg

Lane 3:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 56 kDa

Observed band size: 50 kDa

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