Anti-Glycam1 antibody [EPR30103-507]

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Glycam1 antibody [EPR30103-507] (AB324912)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse lymph node (fresh frozen) tissue labeling Glycam1 with ab324912 at 1/500 (1.01 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). ab307377 was used as a co-localization positive control staining high endothelial venules (magenta).

Panel A : merged staining of anti-GLYCAM1 (ab324912, green), anti-VWF (ab307377, magenta) on mouse lymph node.

Panel B : anti-GLYCAM1 stained on mouse lymph node.

Panel C : anti-VWF stained in high endothelial venules (HEV) of mouse lymph node.

The section was incubated in two rounds of staining : in the order of ab324912 and ab307377, for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Glycam1 antibody [EPR30103-507] (AB324912)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse liver (perfused fixed-fresh frozen) tissue, negative control, labeling Glycam1 with ab324912 at 1/500 (1.01 μg/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 ug/mL) dilution (Green). ab307377 was used as a co-localization positive control (magenta).

Panel A : merged staining of anti-GLYCAM1 (ab324912, green), anti-VWF (ab307377, magenta) on mouse liver.

Panel B : anti-GLYCAM1 stained on mouse liver.

Panel C : anti-VWF stained in endothelial cells in mouse liver.

The section was incubated in two rounds of staining : in the order of ab324912 and ab307377, for 1 hr at room temperature. The nuclear counterstain was DAPI (Blue). The section was then mounted using Fluoromount®.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20)

The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Glycam1 antibody [EPR30103-507] (AB324912)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse lymph node tissue staining Glycam1 with ab324912 at a 1/4000 ( 0.126 μg/ml) dilution, ab237721 anti-CD3 used at 1/2000 dilution ( 0.26 μg/ml) and ab315346 anti-CD37 used at 1/2000 dilution (0.257 μg/ml).

Panel A : anti-Glycam1 (green; Opal™520), anti-CD3 (magenta; Opal™690), anti-CD37 (gray; Opal™570) on mouse lymph node.

Panel B : anti-Glycam1 staining HEV (highly endothelial venules) in mouse lymph node.

Panel C : anti-CD3 staining T lymphocytes in mouse lymph node.

Panel D : anti-CD37 staining B lymphocytes in mouse lymph node.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324912, ab237721 and ab315346 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Glycam1 antibody [EPR30103-507] (AB324912)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse colon tissue staining Glycam1 with ab324912 at a 1/4000 ( 0.126 μg/ml) dilution, ab237721 anti-CD3 used at 1/2000 dilution ( 0.26 μg/ml) and ab315346 anti-CD37 used at 1/2000 dilution (0.257 μg/ml).

Panel A : anti-Glycam1 (green; Opal™520), anti-CD3 (magenta; Opal™690), anti-CD37 (gray; Opal™570) on mouse colon.

Panel B : anti-Glycam1 staining HEV (highly endothelial venules) in mouse colon.

Panel C : anti-CD3 staining T lymphocytes in mouse colon.

Panel D : anti-CD37 staining B lymphocytes in mouse colon.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324912, ab237721 and ab315346 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• mIHC

Lab

Multiplex immunohistochemistry - Anti-Glycam1 antibody [EPR30103-507] (AB324912)

Multiplex immunohistochemistry analysis of formalin/PFA-fixed paraffin-embedded mouse colon tissue staining Glycam1 with ab324912 at a 1/4000 ( 0.126 μg/ml) dilution, ab317313 anti-CD43 used at 1/500 dilution (1.052 μg/ml) and ab272692 anti-MUC2 used at 1/2000 dilution (0.26 μg/ml).

Panel A : anti-Glycam1 (green; Opal™520), anti-CD43 (magenta; Opal™690), anti-MUC2 (gray; Opal™570) on mouse colon.

Panel B : anti-Glycam1 staining HEV (highly endothelial venules) in mouse colon.

Panel C : anti-CD43 staining T lymphocytes in mouse colon.

Panel D : anti-MUC2 staining goblet cells in mouse colon.

Nuclear DNA was labeled with DAPI (shown in blue).

The section was incubated in three rounds of staining : in the order of ab324912, ab237721 and ab315346 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycam1 antibody [EPR30103-507] (AB324912)

Immunohistochemical analysis of paraffin-embedded Mouse breast tissue labeling Glycam1 with ab324912 at 1/4000 (0.126 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse breast. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycam1 antibody [EPR30103-507] (AB324912)

Immunohistochemical analysis of paraffin-embedded Mouse colon tissue labeling Glycam1 with ab324912 at 1/4000 (0.126 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on HEV (highly endothelial venules) in mouse colon. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycam1 antibody [EPR30103-507] (AB324912)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling Glycam1 with ab324912 at 1/4000 (0.126 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse liver. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycam1 antibody [EPR30103-507] (AB324912)

Immunohistochemical analysis of paraffin-embedded Mouse lung tissue labeling Glycam1 with ab324912 at 1/4000 (0.126 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on mouse lung. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycam1 antibody [EPR30103-507] (AB324912)

Immunohistochemical analysis of paraffin-embedded Mouse lymph node tissue labeling Glycam1 with ab324912 at 1/4000 (0.126 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on HEV (highly endothelial venules) of mouse lymph node. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Incubate slides with 3% Hydrogen Peroxide for 10 mins at room temperature after secondary antibody incubation to reduce the background.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins