Anti-Glycine decarboxylase antibody

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (AB204087)

Immunohistochemical analysis of paraffin-embedded human placenta tissue labeling Glycine decarboxylase (high expression) with ab204087 at a 1/1000 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (AB204087)

Immunohistochemical analysis of paraffin-embedded human liver tissue labeling Glycine decarboxylase (high expression) with ab204087 at a 1/1000 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Glycine decarboxylase antibody (AB204087)

Immunocytochemical analysis of HepG2 (Human liver hepatocellular carcinoma cell line) whole cells labeling Glycine decarboxylase in the nucleus and mitochondria with ab204087 at 2 μg/ml.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glycine decarboxylase antibody (AB204087)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Glycine decarboxylase (high expression) with ab204087 at a 1/1000 dilution.

Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

• WB

Supplier Data

Western blot - Anti-Glycine decarboxylase antibody (AB204087)

Loading control : Anti-GAPDH.

All lanes:

Western blot - Anti-Glycine decarboxylase antibody (ab204087) at 0.4 µg/mL

Lane 1:

Caco-2 (Human colorectal adenocarcinoma cell line) whole cell lysate

Lane 2:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate

Predicted band size: 112 kDa

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• WB

Lab

Western blot - Anti-Glycine decarboxylase antibody (AB204087)

Western blot : Rabbit Polyclonal to Glycine decarboxylase ab204087 staining at 0.4 µg/mL, shown in green; Mouse anti-CANX (ab238078) loading control staining at 1/20,000 dilution, shown in magenta. A band was observed at 110 kDa in Wild-type A549 cell lysates with no signal observed at this size in GLDC knockout A549 cell line. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3pc Milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit 800CW & Goat anti-Mouse 680RD at 1/20,000 dilution.

All lanes:

Western blot - Anti-Glycine decarboxylase antibody (ab204087) at 0.4 µg/mL

Lane 1:

Wild-type A549 at 20 µg

Lane 2:

Western blot - Human GLDC knockout A549 cell line (<a href='/ko/products/cell-lines/human-gldc-knockout-a549-cell-line-ab300972'>ab300972</a>) at 20 µg

Lane 3:

HepG2 at 20 µg

Lane 4:

HAP1 at 20 µg

Lane 5:

Raji at 20 µg

Secondary

Lanes 1 - 5:

Goat anti-Rabbit 800CW at 1/20000 dilution

Lanes 1 - 5:

Goat anti-Mouse 680RD at 1/20000 dilution

Predicted band size: 110 kDa

Observed band size: 110 kDa,76 kDa

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