AB255600

Anti-Glypican 1/ GPC1 antibody [EPR22580-72]

20ul selling size
BOND RX™ Validated
RabMAb
Recombinant
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Rabbit Recombinant Monoclonal Glypican 1/ GPC1 antibody. Suitable for IP, WB, IHC-P and reacts with Human samples.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] (AB255600)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] (AB255600)

Immunohistochemical analysis of paraffin-embedded esophagus lung carcinoma tissue labeling Glypican 1/ GPC1 with ab255600 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on esophagus carcinoma (PMID : 28445969) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab255600 for 30 mins at RT.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] (AB255600)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue (A) and the paired adjacent lung tissue (B) labeling Glypican 1/ GPC1 with ab255600 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on human lung carcinoma (panel A) and the paired adjacent lung (panel B) was barely stained (PMID : 29785020) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab255600 for 30 mins at RT.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] (AB255600)

Immunohistochemical analysis of paraffin-embedded human pancreatic carcinoma tissue (A) and the paired adjacent pancreas tissue (B) labeling Glypican 1/ GPC1 with ab255600 at 1/500 dilution, followed by Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Cytoplasmic and membranous staining on human pancreatic carcinoma (panel A) and no staining on the paired adjacent pancreas (panel B, PMID : 28440066) is observed. Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab255600 for 30 mins at RT.

The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

Unknown

Immunoprecipitation - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] (AB255600)

Glypican 1/ GPC1 was immunoprecipitated from 0.35 mg DU 145 (human prostate carcinoma cell line) whole cell lysate 10μg with ab255600 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab255600 1/1000 dilution (0.65 μg/ml). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

Lane 1 : DU 145 (human prostate carcinoma cell line) whole cell lysate 10μg

Lane 2 : ab255600 IP in DU 145 whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab255600 in DU 145 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 75 seconds.

All lanes:

Immunoprecipitation - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] (ab255600)

Predicted band size: 61 kDa

Observed band size: 60 kDa

false

Unknown

Western blot - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] (AB255600)

Blocking and dilution biffer : 5% NFDM/TBST.

Molecular weight observed is consistent with what has been described in the literature (PMID : 8207484, 27313791).

Homology with other Glypican family members (38.88% to GPC-2; 21.55% to GPC-3; 43.41% to GPC-4; 25.86% to GPC-5; 45.05% to GPC-6).

Exposure time :

Lanes 1-2 : 5.5 seconds

Lanes 3-4 : 3 minutes

All lanes:

Western blot - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] (ab255600) at 1/1000 dilution

Lane 1:

DU 145 (human prostate carcinoma cell line) cultured under serum-free conditions, whole cell lysate at 20 µg

Lane 2:

PC-3 (human prostate adenocarcinoma epithelial cell line) whole cell lysate at 20 µg

Lane 3:

MCF7 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Lane 4:

MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/50000 dilution

Predicted band size: 61 kDa

Observed band size: 60 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22580-72

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Glypican 1 (GPC1) also known as glypican-1 is a heparan sulfate proteoglycan anchored to the cell membrane via a glycosylphosphatidylinositol (GPI) anchor. This protein belongs to the glypican family and has an approximate mass of 60 to 70 kDa. GPC1 is found mainly on the surface of cells in the central nervous system as well as in muscle tissues and certain epithelial cells. Its structure facilitates interaction with cell surface receptors and extracellular matrix components playing an important role in cellular communication and signaling processes.

Biological function summary

GPC1 influences the regulation of growth factors and morphogens and it has a significant role in the cell division and growth processes. It is not recognized as part of any larger protein complex but interacts with diverse ligand molecules. Researchers identify GPC1 as an important facilitator of fibroblast growth factors (FGF) and sonic hedgehog (Shh) signaling. Its interaction with molecular partners helps to regulate developmental signals important for proper cellular function and organ development.

Pathways

The contributions of GPC1 are observed in the hedgehog signaling pathway and fibroblast growth factor signaling. GPC1's interaction within the hedgehog pathway is critical for organizing cellular growth and differentiation while its involvement in the FGF pathway supports angiogenesis and wound healing. GPC1 coordinates with other glypican family members and related proteins like FGFR1 to mediate these signaling processes effectively.

Researchers have linked GPC1 with cancer progression and neurodegenerative diseases. Abnormal GPC1 expression is often noted in pancreatic cancer influencing tumor growth and metastasis through its modulation of growth factor signaling. Moreover its role in neurodegenerative conditions such as Alzheimer's disease establishes connections with amyloid-beta peptides and tau proteins contributing to disease pathogenesis. These associations make GPC1 an intriguing target for therapeutic intervention and a focus for ongoing medical research.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cell surface proteoglycan that bears heparan sulfate. Binds, via the heparan sulfate side chains, alpha-4 (V) collagen and participates in Schwann cell myelination (By similarity). May act as a catalyst in increasing the rate of conversion of prion protein PRPN(C) to PRNP(Sc) via associating (via the heparan sulfate side chains) with both forms of PRPN, targeting them to lipid rafts and facilitating their interaction. Required for proper skeletal muscle differentiation by sequestering FGF2 in lipid rafts preventing its binding to receptors (FGFRs) and inhibiting the FGF-mediated signaling.

See full target information GPC1

대체 명칭 보기

Glypican-1, GPC1

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glypican 1/ GPC1 antibody [EPR22580-72]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] (AB255600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] (AB255600)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] (AB255600)

Immunoprecipitation - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] (AB255600)

Western blot - Anti-Glypican 1/ GPC1 antibody [EPR22580-72] (AB255600)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

Product promise