AB246973

Anti-Glypican 4 antibody

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Rabbit Polyclonal Glypican 4 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GPC4 aa 450 to C-terminus.

대체 명칭 보기

UNQ474/PRO937, GPC4, Glypican-4, K-glypican

5 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Glypican 4 antibody (AB246973)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Glypican 4 antibody (AB246973)

PFA-fixed, Triton X-100 permeabilized U-2 OS cells stained for Glypican 4 (green) using ab246973 at 4μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 4 antibody (AB246973)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 4 antibody (AB246973)

Paraffin-embedded human kidney tissue stained for Glypican 4 using ab246973 at 1/20 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 4 antibody (AB246973)

Paraffin-embedded human tonsil tissue stained for Glypican 4 using ab246973 at 1/20 dilution in immunohistochemical analysis. No reactivity in geminal center cells, as expected.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 4 antibody (AB246973)

Paraffin-embedded human liver tissue stained for Glypican 4 using ab246973 at 1/20 dilution in immunohistochemical analysis.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 4 antibody (AB246973)

Paraffin-embedded human pancreas tissue stained for Glypican 4 using ab246973 at 1/20 dilution in immunohistochemical analysis.

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GPC4 aa 450 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

O75487

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/50", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Glypican 4 also known as GPC4 is a heparan sulfate proteoglycan a member of the glypican family. This protein is anchored to the cell membrane and plays a role in cellular processes by attaching to the cell surface via a glycosylphosphatidylinositol (GPI) anchor. Its mass is approximately 60-70 kDa. Glypican 4 is expressed in various tissues including the brain pancreas kidney and skeletal muscle. In these tissues Glypican 4 is involved in cell communication and modulation of signaling activities at the cell surface.

Biological function summary

Glypican 4 influences cell growth differentiation and morphogenesis. It interacts with extracellular ligands and growth factors playing a role in developmental processes. Glypican 4 is often part of a complex with other signaling molecules including Wnt and fibroblast growth factors. These interactions help regulate processes essential for organ development and tissue patterning. Glypican 4 through these molecular associations influences signaling pathways that affect both cellular architecture and function.

Pathways

Glypican 4 plays a role in Wnt signaling and Hedgehog pathways. These pathways are critical for embryonic development and cell fate determination. Within the Wnt signaling pathway Glypican 4 interacts with the Wnt protein modulating its distribution and activity on the cell surface. In the Hedgehog pathway Glypican 4 helps modulate hedgehog proteins like Sonic Hedgehog facilitating their movement and interaction with cell surface receptors.

Glypican 4 is connected to some cancers and metabolic disorders like diabetes. Overexpression or mutations in Glypican 4 can contribute to oncogenesis as it may lead to aberrant activation of signaling pathways making it relevant in cancers like pancreatic and brain tumors. Additionally Glypican 4's role in metabolic pathways links it to insulin-related pathways emphasizing its connection with metabolic disorders such as Type 2 diabetes. Glypican 4 interacts with proteins such as insulin receptors which further highlights its relevance in these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Cell surface proteoglycan that bears heparan sulfate. May be involved in the development of kidney tubules and of the central nervous system (By similarity).

See full target information GPC4

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

International journal of molecular sciences 21: PubMed31973107

2020

The TUSC2 Tumour Suppressor Inhibits the Malignant Phenotype of Human Thyroid Cancer Cells via SMAC/DIABLO Protein.

Applications

Unspecified application

Species

Unspecified reactive species

Raffaela Mariarosaria Mariniello,Francesca Maria Orlandella,Anna Elisa De Stefano,Paola Lucia Chiara Iervolino,Giovanni Smaldone,Neila Luciano,Nara Cervone,Francesco Munciguerra,Silvia Esposito,Peppino Mirabelli,Giuliana Salvatore

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Glypican 4 antibody

Immunocytochemistry/ Immunofluorescence - Anti-Glypican 4 antibody (AB246973)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 4 antibody (AB246973)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 4 antibody (AB246973)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 4 antibody (AB246973)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glypican 4 antibody (AB246973)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise