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AB252634

Anti-GM-CSF antibody [EPR22272-71] - BSA and Azide free (Detector)

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Anti-GM-CSF antibody [EPR22272-71] - BSA and Azide free (Detector) (ab252634) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting GM-CSF in ELISA. Suitable for Human, Monkey.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

대체 명칭 보기

Granulocyte-macrophage colony-stimulating factor, Colony Stimulating Factor 2, Pluripoietin-a

1 이미지
Sandwich ELISA - Anti-GM-CSF antibody [EPR22272-71] - BSA and Azide free (Detector) (AB252634)
  • sELISA

Unknown

Sandwich ELISA - Anti-GM-CSF antibody [EPR22272-71] - BSA and Azide free (Detector) (AB252634)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab174448).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22272-71

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Monkey

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB174448

Human GM-CSF ELISA Kit (CSF2)

Recombinant|SimpleStep

ELISA - Human GM-CSF ELISA Kit (CSF2) (AB174448)

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Primary Antibodies

AB259663

Anti-Progranulin antibody [EPR22207-103] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-Progranulin antibody [EPR22207-103] - BSA and Azide free (Capture) (AB259663)

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Primary Antibodies

AB244917

Anti-FGF21 antibody [EPR20586-14] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-FGF21 antibody [EPR20586-14] - BSA and Azide free (Detector) (AB244917)

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Primary Antibodies

AB317281

Anti-Eotaxin antibody [EPR26619-76] - BSA and Azide free (Detector)

Recombinant|RabMAb

Sandwich ELISA - Anti-Eotaxin antibody [EPR26619-76] - BSA and Azide free (Detector) (AB317281)

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Primary Antibodies

AB308079

Anti-G-CSF antibody [EPR22390-163] - BSA and Azide free (Capture)

RabMAb|Recombinant

Sandwich ELISA - Anti-G-CSF antibody [EPR22390-163] - BSA and Azide free (Capture) (AB308079)

  • 0
  • 0
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Reactivity 정보

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제품 세부 정보

ab252634 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human/Monkey GM-CSF. The recommended pair for sandwich ELISA is:
Capture: ab252575, Human/Monkey GM-CSF Capture Antibody (unconjugated)
Detector: ab252634, Human/Monkey GM-CSF Detector Antibody (unconjugated)
The reference range value is 15.63 - 1000 pg/mL.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GM-CSF also known as Granulocyte-Macrophage Colony-Stimulating Factor is a glycoprotein with a mass of approximately 23 kDa. This protein plays a mechanical role in stimulating the differentiation and proliferation of hematopoietic progenitor cells into granulocytes and macrophages. Its expression occurs in various cell types including macrophages T cells endothelial cells and fibroblasts. GM-CSF interacts with its specific receptor known as CSF2 receptor to exert its effects.
Biological function summary

GM-CSF influences the immune system by enhancing the functional capacity of mature neutrophils macrophages and eosinophils. GM-CSF protein acts alone and not as part of a larger protein complex. It is important in regulating immune responses and inflammation. Due to its role in promoting the survival and activation of these immune cells GM-CSF is important for mounting an effective immune defense in response to infections and other challenges.

Pathways

GM-CSF signaling is integral to the JAK-STAT pathway. This pathway is essential for transmitting signals from surface receptors like GM-CSF receptors into the nucleus thereby inducing gene expression that leads to cell survival and proliferation. GM-CSF also intersects with the ERK1/ERK2 MAPK pathway which is involved in regulating cellular processes such as division and differentiation. The GM-CSF receptor shares similarities with other cytokine receptors involved in these pathways making it related to various cytokines through its downstream signaling effects.

GM-CSF has a well-established connection to diseases like rheumatoid arthritis and multiple sclerosis. In rheumatoid arthritis overproduction of GM-CSF is linked to chronic inflammation and joint damage. In multiple sclerosis GM-CSF might contribute to the pathological immune responses against the central nervous system. Antibodies targeting GM-CSF or its receptor represent a therapeutic strategy for these autoimmune diseases. By modulating GM-CSF activity therapeutic approaches aim to reduce inflammation and autoimmunity highlighting the protein's importance in disease progression.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

See full target information CSF2
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Product promise

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