Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker

• ICC/IF

Collaborator

Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (AB30637)

The image shows staining of Drosophila S2 cells using ab30637 to cis-Golgi/Golgi marker, GM130. For comparison, S2 cells were stained with an antibody to the trans-Golgi marker, GCC88. Gm130 stained the cis-Golgi region and appears to be a superior Golgi marker to GCC88.

This image is courtesy of Sean Munro, MRC Laboratory of Molecular Biology

• ICC/IF

PubMed

Immunocytochemistry/ Immunofluorescence - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (AB30637)

ab30637 at a 1/100 dilution staining Golgi/Cis-Golgi in Drosophila S2R+ cells by Immunocytochemistry/ Immunofluorescence.

Expression of recombinant wild type pgant3 (pIB-pgant3-V5), pgant3m1 (pIB-m1-V5), and pgant3m2 (pIB-m2-V5) in Drosophila S2R+ cells. Cells were then stained for V5 and the Golgi marker GM130 revealing Golgi localization of wild type pgant3 and pgant3m1.

Image from Zhang L et al, J Biol Chem. 2010 Jun 18;285(25):19491-501. Epub 2010 Apr 6, Fig 1. DOI 10.1074/jbc.M109.098145 June 18, 2010 The Journal of Biological Chemistry, 285, 19491-19501.

• WB

Unknown

Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (AB30637)

All lanes:

Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 0.1 µg/mL

Lane 1:

Drosophila melanogaster S2 cell extract

Lane 2:

Drosophila melanogaster S2 cell extract + specific siRNA

Predicted band size: 113 kDa

Observed band size: 95 kDa

false

This image is courtesy of Sean Munro, MRC Laboratory of Molecular Biology

• WB

Unknown

Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (AB30637)

All lanes:

Western blot - Anti-GM130 antibody - Drosophila Golgi/Cis-Golgi Marker (ab30637) at 1 µg/mL

Lane 1:

Schneider L2 whole cell lysate (<a href='/ko/products/unavailable/schneider-l2-whole-cell-lysate-ab14893'>ab14893</a>) at 10 µg

Lane 2:

Schneider L2 whole cell lysate (<a href='/ko/products/unavailable/schneider-l2-whole-cell-lysate-ab14893'>ab14893</a>) at 10 µg with <em>D. melanogaster</em> GM130 peptide (<a href='/ko/products/unavailable/d-melanogaster-gm130-peptide-ab32335'>ab32335</a>)

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 113 kDa

Observed band size: 105 kDa,70 kDa

false