AB224322

Anti-GMFB antibody

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(3 제품이 사용된 논문 )

Rabbit Polyclonal GMFB antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Mouse, Human, Rat samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GMFB aa 1 to C-terminus.

대체 명칭 보기

Glia maturation factor beta, GMF-beta, GMFB

12 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Paraffin-embedded human hippocampus tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-GMFB antibody (AB224322)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GMFB antibody (AB224322)

PFA-fixed/Triton X-100 permeabilized U-251 MG (human brain glioma cell line) cells stained for GMFB (green) using ab224322 at 4 μg/ml in ICC/IF.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Paraffin-embedded human cerebral cortex tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Paraffin-embedded mouse lateral septum tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Paraffin-embedded mouse cerebellum tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Paraffin-embedded mouse somatosensory cortex tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Paraffin-embedded mouse olfactory bulb tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Paraffin-embedded mouse red nucleus tissue stained for GMFB using ab224322 at 1/200 dilution in immunohistochemical analysis using a fluorescent secondary antibody.

Supplier Data

Western blot - Anti-GMFB antibody (AB224322)

All lanes:

Western blot - Anti-GMFB antibody (ab224322) at 1/100 dilution

All lanes:

Human cerebral cortex tissue lysate

Predicted band size: 17 kDa

true

Supplier Data

Western blot - Anti-GMFB antibody (AB224322)

All lanes:

Western blot - Anti-GMFB antibody (ab224322) at 1/100 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast cell line) cell lysate

Lane 2:

NBT-II cell lysate

Predicted band size: 17 kDa

true

Supplier Data

Western blot - Anti-GMFB antibody (AB224322)

All lanes:

Western blot - Anti-GMFB antibody (ab224322) at 1/100 dilution

All lanes:

A431 (human epidermoid carcinoma cell line) cell lysate

Predicted band size: 17 kDa

true

Supplier Data

Western blot - Anti-GMFB antibody (AB224322)

All lanes:

Western blot - Anti-GMFB antibody (ab224322) at 1/100 dilution

All lanes:

Mouse cerebral cortex tissue lysate

Predicted band size: 17 kDa

true

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human GMFB aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P60983

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The Glia Maturation Factor Beta (GMFB) is an important regulator in brain development and maintenance. It is also known by the name GMF-b. This protein weighs approximately 17.2 kDa and is expressed in glial cells of the central nervous system as well as other tissues including skin and kidney. By stimulating differentiation and maturation of glial cells GMFB influences central nervous system homeostasis and glial responses to injury.

Biological function summary

GMFB influences numerous cellular processes such as brain maturation and injury repair. It does not form part of a complex but functions independently to exert influence on various targets. GMFB aids in the reorganization of the actin cytoskeleton which is important for cell shape and motility. It regulates neural signaling by interacting with signaling molecules enabling adaptability and cellular communication.

Pathways

GMFB significantly affects neurotrophic and inflammatory pathways. It interacts with proteins like PI3K and AKT to modulate neural survival and plasticity impacting brain health and response to stress. The protein also involves itself in inflammation-related pathways supporting cytokine production and immune responses during injury and infection. Through these interactions GMFB ensures optimal cellular function and organismal health.

GMFB has links to neurodegenerative diseases and gliomas. Abnormal GMFB expression connects with Alzheimer's disease affecting neural health and functionality. In the context of gliomas GMFB influences tumor progression and microenvironment implicating it in tumorigenesis. In these disorders GMFB interacts with proteins such as GFAP and beta-amyloid leading to dysregulation in cellular signaling and pathological developments.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

This protein causes differentiation of brain cells, stimulation of neural regeneration, and inhibition of proliferation of tumor cells.

See full target information GMFB

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of neuroinflammation 20:177 PubMed37507761

2023

Cellulose ether treatment inhibits amyloid beta aggregation, neuroinflammation and cognitive deficits in transgenic mouse model of Alzheimer's disease.

Applications

Unspecified application

Species

Unspecified reactive species

Tahir Ali,Antonia N Klein,Keegan McDonald,Lovisa Johansson,Priyanka Ganguli Mukherjee,Martin Hallbeck,Katsumi Doh-Ura,Hermann M Schatzl,Sabine Gilch

Molecular neurobiology 56:378-393 PubMed29704201

2018

Targeted Gene Editing of Glia Maturation Factor in Microglia: a Novel Alzheimer's Disease Therapeutic Target.

Applications

Unspecified application

Species

Unspecified reactive species

Sudhanshu P Raikwar,Ramasamy Thangavel,Iuliia Dubova,Govindhasamy Pushpavathi Selvakumar,Mohammad Ejaz Ahmed,Duraisamy Kempuraj,Smita A Zaheer,Shankar S Iyer,Asgar Zaheer

Journal of Alzheimer's disease : JAD 61:553-560 PubMed29172001

2017

Co-Expression of Glia Maturation Factor and Apolipoprotein E4 in Alzheimer's Disease Brain.

Applications

IHC-P

Species

Unspecified reactive species

Ramasamy Thangavel,Sachin M Bhagavan,Swathi Beladakere Ramaswamy,Spurthi Surpur,Raghav Govindarajan,Duraisamy Kempuraj,Smita Zaheer,Sudhanshu Raikwar,Mohammad E Ahmed,Govindhasamy Pushpavathi Selvakumar,Shankar S Iyer,Asgar Zaheer

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GMFB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Immunocytochemistry/ Immunofluorescence - Anti-GMFB antibody (AB224322)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GMFB antibody (AB224322)

Western blot - Anti-GMFB antibody (AB224322)

Western blot - Anti-GMFB antibody (AB224322)

Western blot - Anti-GMFB antibody (AB224322)

Western blot - Anti-GMFB antibody (AB224322)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (3)

Product promise