Anti-GNAS antibody

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GNAS antibody (AB235959)

4% paraformaldehyde-fixed, 0.2% Triton X-100-permeabilized MCF7 (Human breast adenocarcinoma cell line) cells stained for G protein alpha S (Green) using ab235959 at 1/200 dilution in ICC/IF. Counterstained with DAPI (blue). The secondary antibody was Alexa Fluor^® 488-conjugated Goat Anti-Rabbit IgG (H+L).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAS antibody (AB235959)

Paraffin-embedded human brain tissue stained for G protein alpha S using ab235959 at 1/600 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNAS antibody (AB235959)

Paraffin-embedded human small intestine tissue stained for G protein alpha S using ab235959 at 1/600 dilution in immunohistochemical analysis.

After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 minutes at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized tissue using an HRP conjugated SP system.