AB244403

Anti-GNE antibody

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Rabbit Polyclonal GNE antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human GNE aa 250-450.

대체 명칭 보기

GLCNE, IBM2, GNE, Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase, UDP-GlcNAc-2-epimerase/ManAc kinase

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-GNE antibody (AB244403)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GNE antibody (AB244403)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for GNE (green) using ab244403 at 4 μg/ml in ICC/IF.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNE antibody (AB244403)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNE antibody (AB244403)

Paraffin-embedded human liver tissue stained for GNE using ab244403 at 1/50 dilution in immunohistochemical analysis.

Unknown

Western blot - Anti-GNE antibody (AB244403)

All lanes:

Western blot - Anti-GNE antibody (ab244403) at 0.4 µg/mL

All lanes:

A549 (Human lung carcinoma cell line) cell lysate

Predicted band size: 79 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human GNE aa 250-450. The exact immunogen used to generate this antibody is proprietary information.

Q9Y223

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chinese hamster": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GNE also called glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase is a bifunctional enzyme with a mass of approximately 82 kDa. This enzyme catalyzes the first two steps in sialic acid biosynthesis. GNE expression occurs in many tissues highest in liver and kidney and also in heart muscle and brain. Its bifunctional nature arises from two domain activities that facilitate the essential conversion of UDP-N-acetylglucosamine to N-acetylneuraminic acid a precursor for sialic acids.

Biological function summary

Glucosamine (UDP-N-acetyl)-2-epimerase/N-acetylmannosamine kinase acts as an important enzyme in sialic acid production acting alone and not part of a larger enzymatic complex. Sialic acids are terminal sugars on glycoproteins and glycolipids influencing cell-cell interaction signaling and stability. The enzyme's function affects cell surface structures impacting processes like cell migration and immune response.

Pathways

The enzyme GNE plays an important role in the sialylation pathway affecting various biological systems. This pathway involves the modification of glycoproteins and glycolipids critical for maintaining cellular communication. UDP-N-acetylglucosamine and N-acetylneuraminic acid are intermediates in pathways that also involve proteins like CMP-sialic acid transporter which mediates the transport of sialic acids into the Golgi apparatus for post-translational modification processes.

Defects in GNE are linked to conditions such as hereditary inclusion body myopathy (HIBM) an autosomal recessive muscle-wasting disorder. Another associated condition is sialuria a rare metabolic disorder tied to excessive levels of sialic acid. In HIBM mutations affect the enzyme’s kinase activity leading to reduced sialylation of glycoproteins. Research shows a relationship between GNE and proteins like sialin whose function as a transporter can impact the pathological manifestation of these disorders.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Bifunctional enzyme that possesses both UDP-N-acetylglucosamine 2-epimerase and N-acetylmannosamine kinase activities, and serves as the initiator of the biosynthetic pathway leading to the production of N-acetylneuraminic acid (NeuAc), a critical precursor in the synthesis of sialic acids. By catalyzing this pivotal and rate-limiting step in sialic acid biosynthesis, this enzyme assumes a pivotal role in governing the regulation of cell surface sialylation, playing a role in embryonic angiogenesis (PubMed : 10334995, PubMed : 11326336, PubMed : 14707127, PubMed : 16503651, PubMed : 2808337, PubMed : 38237079). Sialic acids represent a category of negatively charged sugars that reside on the surface of cells as terminal components of glycoconjugates and mediate important functions in various cellular processes, including cell adhesion, signal transduction, and cellular recognition (PubMed : 10334995, PubMed : 14707127).

See full target information GNE

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Regenerative therapy 22:19-29 PubMed36582605

2022

Cancer stem cell-like cells-derived exosomal lncRNA promotes biological characteristics in thyroid cancer via miR-122-5p/P4HA1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Qinghua Wu,Yonggang He,Xin Liu,Fangxiu Luo,Yimei Jiang,Ming Xiang,Ren Zhao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GNE antibody

Immunocytochemistry/ Immunofluorescence - Anti-GNE antibody (AB244403)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GNE antibody (AB244403)

Western blot - Anti-GNE antibody (AB244403)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise