JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB224040

Anti-GOLGA5/Golgin-84 antibody

Be the first to review this product! 리뷰 제출

|

(3 제품이 사용된 논문 )

Rabbit Polyclonal GOLGA5/Golgin-84 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GOLGA5 aa 150 to C-terminus.

대체 명칭 보기

RETII, RFG5, PIG31, GOLGA5, Golgin subfamily A member 5, Cell proliferation-inducing gene 31 protein, Golgin-84, Protein Ret-II, RET-fused gene 5 protein

7 이미지
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB224040)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB224040)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebellum tissue labelling GOLGA5/Golgin-84 with ab224040 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB224040)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB224040)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human epididymis tissue labelling GOLGA5/Golgin-84 with ab224040 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB224040)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB224040)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling GOLGA5/Golgin-84 with ab224040 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB224040)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB224040)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling GOLGA5/Golgin-84 with ab224040 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB224040)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GOLGA5/Golgin-84 antibody (AB224040)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach tissue labelling GOLGA5/Golgin-84 with ab224040 at 1/200 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-GOLGA5/Golgin-84 antibody (AB224040)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GOLGA5/Golgin-84 antibody (AB224040)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for GOLGA5/Golgin-84 (green) using ab224040 at 4 μg/ml in ICC/IF.

Western blot - Anti-GOLGA5/Golgin-84 antibody (AB224040)
  • WB

Supplier Data

Western blot - Anti-GOLGA5/Golgin-84 antibody (AB224040)

Loading control : GAPDH

All lanes:

Western blot - Anti-GOLGA5/Golgin-84 antibody (ab224040) at 0.4 µg/mL

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cells transfected with control siRNA, whole cell lysate

Lane 2:

U-87MG cells transfected with target specific siRNA probe #1, whole cell lysate

Lane 3:

U-87MG cells transfected with target specific siRNA probe #2, whole cell lysate

Predicted band size: 83 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human GOLGA5 aa 150 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8TBA6

style
left-column

대체품

Primary Antibodies

AB155806

Anti-GOLGA5/Golgin-84 antibody

Immunocytochemistry/ Immunofluorescence - Anti-GOLGA5/Golgin-84 antibody (AB155806)

  • 0
  • 0

보완 제품

Primary Antibodies

AB174834

Anti-GRASP65 antibody [EPR12439] - C-terminal

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRASP65 antibody [EPR12439] - C-terminal (AB174834)

  • 5
  • 4
Primary Antibodies

AB186735

Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker

RabMAb|Recombinant|Lab Essentials|20ul selling size

Western blot - Anti-TOMM20 antibody [EPR15581-54] - Mitochondrial Marker (AB186735)

  • 5
  • 16
Primary Antibodies

AB115321

Anti-pan PDK antibody [3H5BE12]

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-pan PDK antibody [3H5BE12] (AB115321)

  • 5
  • 4
Primary Antibodies

AB232928

Anti-Salusin alpha antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Salusin alpha antibody (AB232928)

  • 0
  • 0
style
left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
style
left-column
style
left-column

특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Immunogen
보관 버퍼
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
style
left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GOLGA5 also known as Golgin-84 is an important player in maintaining the structural integrity of the Golgi apparatus. This protein weighs approximately 84 kDa and exists within the Golgi membrane structure. It facilitates the tethering and fusion of vesicles ensuring the proper flow of proteins and lipids through the Golgi network. Golgin-84 expresses in a variety of tissues with strong local presence in cells undergoing secretion indicating its role in cellular transport processes.
Biological function summary

GOLGA5 participates in the assembly of the Golgi ribbon a structure essential for the efficient functioning of the Golgi apparatus. This protein integrates into a multiprotein complex which is critical for maintaining the stacked and ribbon-like structure of the Golgi. Its interaction with other Golgi matrix proteins helps regulate the positioning and coherence of Golgi cisternae. This ensures proper vesicle transfer and contributes to overall cellular homeostasis.

Pathways

The function of GOLGA5 is critical in the secretory pathway where it ensures the orderly transport of proteins processed by the Golgi apparatus. The presence of GOLGA5 supports Golgi reassembly and stacking protein 65 (GRASP65) involvement in Golgi reformation during the cell cycle. Its function also connects with ER-to-Golgi transport helping maintain an important support system for intracellular trafficking.

GOLGA5 appears related to neurodegenerative diseases and cancer. Alterations in its function or expression could dispose cells to develop cellular transport disruptions potentially leading to illnesses like Alzheimer’s disease where protein transport and processing are compromised. Furthermore in cases of cancer such as prostate cancer changes in GOLGA5 expression might disrupt normal cell function linking it to pathways involving oncoproteins.
style
left-column
style
left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

타겟 정보

Involved in maintaining Golgi structure. Stimulates the formation of Golgi stacks and ribbons. Involved in intra-Golgi retrograde transport.
See full target information GOLGA5
style
left-column

제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of neuroinflammation 19:43 PubMed35135580

2022

PD-L1 signaling in reactive astrocytes counteracts neuroinflammation and ameliorates neuronal damage after traumatic brain injury.

Applications

Unspecified application

Species

Unspecified reactive species

Xiang Gao,Wei Li,Fahim Syed,Fang Yuan,Ping Li,Qigui Yu

Journal of neuroscience methods 361:109285 PubMed34242703

2021

Protein-retention expansion microscopy for visualizing subcellular organelles in fixed brain tissue.

Applications

Unspecified application

Species

Unspecified reactive species

Logan A Campbell,Katy E Pannoni,Niesha A Savory,Dinesh Lal,Shannon Farris

Frontiers in cellular and infection microbiology 11:675890 PubMed34169005

2021

Akt Phosphorylation Influences Persistent Chlamydial Infection and -Induced Golgi Fragmentation Without Involving Rab14.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaobao Huang,Jinfeng Tan,Xiaohong Chen,Mingna Liu,Huiling Zhu,Wenjing Li,Zhenjian He,Jiande Han,Chunguang Ma
제품이 사용된 논문 모두 보기
style
left-column
style
left-column
style
right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com