AB251810

Anti-GPBB antibody

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Rabbit Polyclonal GPBB antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Glycogen phosphorylase, brain form aa 650 to C-terminus.

대체 명칭 보기

PYGB

3 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPBB antibody (AB251810)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPBB antibody (AB251810)

Paraffin-embedded human prostate tissue stained for GPBB using ab251810 at 1/1000 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-GPBB antibody (AB251810)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GPBB antibody (AB251810)

PFA-fixed, Triton X-100 permeabilized PC-3 (human prostate adenocarcinoma cell line) cells stained for GPBB (green) using ab251810 at 4 µg/ml in ICC/IF.

Supplier Data

Western blot - Anti-GPBB antibody (AB251810)

All lanes:

Western blot - Anti-GPBB antibody (ab251810) at 0.4 µg/mL

Lane 1:

SK-MEL-30 cell line

Lane 2:

Human epithelial cell line from cervix adenocarcinoma (HeLa cell line)

Predicted band size: 96 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human Glycogen phosphorylase, brain form aa 650 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P11216

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000 - 1/2500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "Fixation/Permeabilization: PFA/Triton X-100." }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The protein generally known as GPBB (glycogen phosphorylase brain form) plays a critical role in glucose metabolism. An alternate name for this protein is brain-type glycogen phosphorylase. The GPBB protein weighs approximately 97 kDa. It is mainly expressed in the brain but also present in skeletal and cardiac muscle tissues. This enzyme catalyzes the breakdown of glycogen into glucose-1-phosphate an essential step in energy production particularly during periods of increased cellular energy demand.

Biological function summary

GPBB contributes significantly to cellular energy homeostasis. It often partners with AMP-activated protein kinase (AMPK) among other proteins acting as a part of cellular complexes that regulate energy metabolism. GPBB is essential in maintaining the balance between glycogen synthesis and degradation ensuring that glucose levels rise during energy-demanding activities. This regulation supports proper brain function especially in neurons which heavily rely on glucose as an energy source.

Pathways

GPBB is fundamental in the glycogenolysis pathway which mobilizes glucose from glycogen reserves during metabolic needs. GPBB also relates to the insulin signaling pathway influencing how cells respond to insulin a protein vital for glucose uptake and metabolism. Through these pathways GPBB interacts with other proteins like glycogen synthase and phosphoglucomutase establishing a network that carefully coordinates metabolic responses to varying energy availability.

GPBB has significant implications in cardiac ischemia and neurodegenerative disorders such as Alzheimer's disease. During cardiac ischemia elevated levels of GPBB often indicate heart damage given its role in glycogen breakdown in cardiac muscle cells. In Alzheimer's disease abnormal GPBB activity might affect neuronal energy balance contributing to the disease's progression. Understanding these connections is important for developing therapeutic approaches targeting metabolic pathways involving GPBB.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Glycogen phosphorylase that regulates glycogen mobilization (PubMed : 27402852). Phosphorylase is an important allosteric enzyme in carbohydrate metabolism (PubMed : 3346228). Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates (PubMed : 3346228). However, all known phosphorylases share catalytic and structural properties (PubMed : 3346228).

See full target information Glycogen phosphorylase, brain form

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Bioscience reports 40: PubMed32880385

2020

Identification and validation of a prognostic index based on a metabolic-genomic landscape analysis of ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Qun-Feng Zhang,Yu-Kun Li,Chang-Ye Chen,Xiao-di Zhang,Lu Cao,Fei-Fei Quan,Xin Zeng,Juan Wang,Jue Liu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPBB antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPBB antibody (AB251810)

Immunocytochemistry/ Immunofluorescence - Anti-GPBB antibody (AB251810)

Western blot - Anti-GPBB antibody (AB251810)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise