AB75312

Anti-GPCR GPR119 antibody

Name: Anti-GPCR GPR119 antibody
Brand: Abcam Limited
SKU: AB75312
Rating: 4 (2 reviews)

(2 리뷰들)

(3 제품이 사용된 논문 )

Rabbit Polyclonal GPCR GPR119 antibody. Suitable for ELISA, WB, IHC-P, ICC/IF, IHC-Fr and reacts with Mouse, Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human GPR119 aa 200-250.

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR119 antibody (AB75312)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR119 antibody (AB75312)

Left panel : immunofluorescence analysis of MCF-7 cells, using 1/500 ab75312. Right panel : same, after pre-incubation with the immunizing peptide.

Unknown

Western blot - Anti-GPCR GPR119 antibody (AB75312)

All lanes:

Western blot - Anti-GPCR GPR119 antibody (ab75312) at 1/500 dilution

Lane 1:

extracts from K562 cells at 30 µg

Lane 2:

extracts from K562 cells at 30 µg with immunising peptide

Predicted band size: 36 kDa

Observed band size: 28 kDa,37 kDa,60 kDa

false

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human, Mouse

Applications

ICC/IF, ELISA, IHC-Fr, WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human GPR119 aa 200-250. The exact immunogen used to generate this antibody is proprietary information.

Q8TDV5

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GPR119 also known as Glucose-dependent insulinotropic receptor is a G protein-coupled receptor (GPCR) that plays an essential role in metabolic regulation. With a mass of approximately 51 kDa it is expressed mainly in pancreatic beta cells and intestinal enteroendocrine cells. This receptor senses dietary lipids and promotes insulin secretion in response to meals. It also couples with G proteins to initiate intracellular signaling cascades important for energy homeostasis.

Biological function summary

GPR119 functions as a sensor of lipid-derived molecules often acting as an aryl agonist receptor. It is not part of a larger complex but can activate a series of downstream pathways upon ligand binding. When activated by endogenous and synthetic agonists GPR119 stimulates the release of incretin hormones such as GLP-1 and GIP. This hormone release enhances insulin response and promotes optimal glycemic control essential in maintaining blood glucose levels.

Pathways

GPR119 signaling pathway is integral in glucose metabolism and energy balance. It links to both the incretin hormone signaling pathway and the insulin signaling pathway. Upon activation it influences the levels of cAMP an important secondary messenger that modulates several downstream effects including the regulation of the insulin receptor. GPR119 interacts with other proteins such as adenylate cyclase affecting insulin secretion and lipid metabolism.

GPR119 has significant relevance to type 2 diabetes and obesity. Its role in glucose homeostasis and energy balance makes it a potential therapeutic target for these conditions. Researchers examine GPR119 agonists as possible treatments for enhancing insulin secretion and controlling body weight. The receptor is linked to related pathways integrating multiple metabolic signals including those mediated by GLP-1 an important hormone involved in energy regulation and glycemic control.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Receptor for the endogenous fatty-acid ethanolamide oleoylethanolamide (OEA) and lysophosphatidylcholine (LPC). Functions as a glucose-dependent insulinotropic receptor. The activity of this receptor is mediated by G proteins which activate adenylate cyclase. Seems to act through a G(s) mediated pathway.

See full target information GPR119

대체 명칭 보기

Glucose-dependent insulinotropic receptor, G-protein coupled receptor 119, GPR119

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제품이 사용된 논문 (3)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell proliferation 58:e13764 PubMed39413003

2024

Terazosin, a repurposed GPR119 agonist, ameliorates mitophagy and β-cell function in NAFPD by inhibiting MST1-Foxo3a signalling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chenglei Zhang,Jiarui Li,Lijuan Wang,Jie Ma,Xin Li,Yuanyuan Wu,Yanru Ren,Yanhui Yang,Hui Song,Jianning Li,Yi Yang

The Journal of investigative dermatology 140:1909-1918.e8 PubMed32142797

2020

GPR119 Is a Potent Regulator of Human Sebocyte Biology.

Applications

Unspecified application

Species

Unspecified reactive species

Arnold Markovics,Ágnes Angyal,Kinga Fanni Tóth,Dorottya Ádám,Zsófia Pénzes,József Magi,Ágnes Pór,Ilona Kovács,Dániel Törőcsik,Christos C Zouboulis,Tamás Bíró,Attila Oláh

American journal of physiology. Gastrointestinal a 307:G958-67 PubMed25258406

2014

Characterization of duodenal expression and localization of fatty acid-sensing receptors in humans: relationships with body mass index.

Applications

Unspecified application

Species

Unspecified reactive species

Tanya J Little,Nicole J Isaacs,Richard L Young,Raffael Ott,Nam Q Nguyen,Christopher K Rayner,Michael Horowitz,Christine Feinle-Bisset

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPCR GPR119 antibody

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR119 antibody (AB75312)

Western blot - Anti-GPCR GPR119 antibody (AB75312)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (3)

Product promise