AB79516

Anti-GPCR GPR32 antibody

Name: Anti-GPCR GPR32 antibody
Brand: Abcam Limited
SKU: AB79516
Rating: 4 (2 reviews)

(2 리뷰들)

(4 제품이 사용된 논문 )

Rabbit Polyclonal GPCR GPR32 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human GPR32.

2 이미지

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR32 antibody (AB79516)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR32 antibody (AB79516)

Left panel : immunofluorescence analysis of A549 cells using ab79516 at a 1/500 dilution. Right panel : same, after pre-incubation with the immunizing peptide.

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Western blot - Anti-GPCR GPR32 antibody (AB79516)

All lanes:

Western blot - Anti-GPCR GPR32 antibody (ab79516) at 1/500 dilution

Lane 1:

Extracts from A549 cells at 15 µg

Lane 2:

Extracts from A549 cells at 15 µg with immunizing peptide

Predicted band size: 40 kDa

Observed band size: 33 kDa

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주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human GPR32. The exact immunogen used to generate this antibody is proprietary information.

O75388

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "<p></p>" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

보관 버퍼

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

배송 시 보관 조건

Blue Ice

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

G protein-coupled receptor 32 (GPR32) also known as GPR32 is a member of the G protein-coupled receptors (GPCRs) family characterized by its seven-transmembrane domain structure. The molecular mass of GPR32 is approximately 40 kDa. Researchers typically find GPR32 expression in human tissues such as lung and heart. The receptor interacts with specific ligands activating intracellular signaling pathways that lead to various cellular responses.

Biological function summary

GPR32 plays a role in the immune system regulation and inflammation resolution. It partners with ligand molecules like certain specialized pro-resolving mediators (SPMs) to facilitate anti-inflammatory responses. Scientists study GPR32 as part of the broader network of GPCRs known for their involvement in transmitting signals from the extracellular environment into the cell. By influencing resolution pathways GPR32 helps maintain tissue homeostasis.

Pathways

GPR32 significantly influences the resolution phase of inflammation and integrates into the SPM signaling pathway. It interacts with other proteins such as the ALX/FPR2 receptor which is also known to mediate anti-inflammatory actions. Through these interactions GPR32 contributes to orchestrating inflammatory pathway modulation a critical process for balancing immune responses and promoting tissue repair.

GPR32 has a reported relation with inflammatory conditions and cardiovascular diseases. Researchers have studied its role in chronic inflammation where disrupted regulation might contribute to pathological inflammation persistence. GPR32's involvement in cardiovascular diseases links it to the regulation of blood pressure and vascular inflammation with potential interactions seen with proteins such as NADPH oxidase known to affect oxidative stress within these conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

G-protein coupled receptor that binds to several ligands including resolvin D1 (RvD1) with high affinity, leading to rapid and transient activation of numerous intracellular signaling pathways. In macrophages, enhances the RvD1-stimulated phagocytic and clearance functions (PubMed : 20080636). Macrophages migrate less toward different chemoattractant stimuli but phagocytose more microbial particles (PubMed : 26969756). Prevents the increase in Ca(2+) and activation of ERK1/2 used by histamine and its H1 receptor subtype to induce goblet cell secretion by activating PKC and GRK2 to counter-regulate the histamine receptor (PubMed : 23462912).

See full target information GPR32

대체 명칭 보기

Probable G-protein coupled receptor 32, GPR32

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제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록 and refine your search

In vitro cellular & developmental biology. Animal 61:331-339 PubMed40064746

2025

Resolvin D1 suppresses inflammation in human fibroblast-like synoviocytes via the p-38, NF-κB, and AKT signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Makoto Yanoshita,Naoto Hirose,Sayuri Nishiyama,Eri Tsuboi,Naoki Kubo,Daiki Kita,Kotaro Tanimoto

JCI insight 6: PubMed34166226

2021

Inflammation resolution circuits are uncoupled in acute sepsis and correlate with clinical severity.

Applications

Unspecified application

Species

Unspecified reactive species

Bakr Jundi,Do-Hyun Lee,Hyungkook Jeon,Melody G Duvall,Julie Nijmeh,Raja-Elie E Abdulnour,Mayra Pinilla-Vera,Rebecca M Baron,Jongyoon Han,Joel Voldman,Bruce D Levy

Journal of thrombosis and haemostasis : JTH 15:802-813 PubMed28079976

2017

Maresin 1 induces a novel pro-resolving phenotype in human platelets.

Applications

Unspecified application

Species

Unspecified reactive species

K L Lannan,S L Spinelli,N Blumberg,R P Phipps

Journal of immunology (Baltimore, Md. : 1950) 193:4980-7 PubMed25320283

2014

Resolvin D1 attenuates polyinosinic-polycytidylic acid-induced inflammatory signaling in human airway epithelial cells via TAK1.

Applications

Unspecified application

Species

Unspecified reactive species

Hsi-Min Hsiao,Thomas H Thatcher,Elizabeth P Levy,Robert A Fulton,Kristina M Owens,Richard P Phipps,Patricia J Sime

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPCR GPR32 antibody

Immunocytochemistry/ Immunofluorescence - Anti-GPCR GPR32 antibody (AB79516)

Western blot - Anti-GPCR GPR32 antibody (AB79516)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (4)

Product promise