AB188585

Anti-GPD2 antibody [EPR14259]

Name: Anti-GPD2 antibody [EPR14259]
Brand: Abcam Limited
SKU: AB188585
Rating: 4 (3 reviews)

RabMAb
Recombinant
각 태그의 뜻

(3 리뷰들)

(15 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal GPD2 antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 15 publications.

대체 명칭 보기

GPD-M, GPDH-M, mitochondrial glycerophosphate dehydrogenase gene, mtGPD, mGDH, GPD2

3 이미지

Flow Cytometry (Intracellular) - Anti-GPD2 antibody [EPR14259] (AB188585)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-GPD2 antibody [EPR14259] (AB188585)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling GPD2 with ab188585 at 1/310 dilution (red) compared to a rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

Supplier Data

Immunoprecipitation - Anti-GPD2 antibody [EPR14259] (AB188585)

Western blot aalysis of GPD2 in HeLa cell lysate immunoprecipitated using ab188585 at 1/50 dilution.

Secondary antibody : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-GPD2 antibody [EPR14259] (ab188585)

Predicted band size: 81 kDa

false

Supplier Data

Western blot - Anti-GPD2 antibody [EPR14259] (AB188585)

All lanes:

Western blot - Anti-GPD2 antibody [EPR14259] (ab188585) at 1/10000 dilution

Lane 1:

HeLa cell lysate at 20 µg

Lane 2:

MCF-7 cell lysate at 20 µg

Lane 3:

NIH:OVCAR-3 cell lysate at 20 µg

Lane 4:

U87-MG cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 81 kDa

Observed band size: 74 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR14259

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30 - 1/50", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/310", "FlowCytIntra-species-notes": "<a href='/ko/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Glycerol-3-phosphate dehydrogenase 2 (GPD2) also known as GPDM-m and mGPDH is an enzyme that belongs to the glycerol-3-phosphate dehydrogenase family. GPD2 weighs approximately 74 kDa and is expressed in several tissues including the liver kidney and muscle. It primarily localizes in the mitochondrial inner membrane. Mechanically GPD2 catalyzes the oxidation of glycerol-3-phosphate to dihydroxyacetone phosphate playing an important role in energy metabolism by participating in the glycerol phosphate shuttle. This shuttle facilitates the transfer of reducing equivalents across the mitochondrial membrane.

Biological function summary

GPD2 plays a role in cellular energy production by linking lipid metabolism to carbohydrate metabolism. It forms part of a complex system supporting oxidative phosphorylation by transferring electrons to the mitochondrial electron transport chain. The activity of GPD2 helps sustain the NAD+/NADH balance which is essential for maintaining cellular redox states. Through its function GPD2 contributes to metabolic flexibility allowing cells to adapt to changes in fuel availability.

Pathways

GPD2 is involved in important energy production pathways such as the glycerol phosphate shuttle and the oxidative phosphorylation pathway. These pathways are important for efficient ATP production in eukaryotic cells. GPD2 interacts with other metabolic proteins like cytosolic glycerol-3-phosphate dehydrogenase (GPD1) which works in tandem to facilitate the shuttle of reducing equivalents between cytosol and mitochondria. Such processes ensure continuous energy supply necessary for cellular functions especially under conditions where carbohydrates and lipids serve interchangeably as energy sources.

GPD2 associates with conditions such as obesity and type 2 diabetes where energy metabolism is often disrupted. Altered GPD2 activity can lead to impaired mitochondrial function contributing to the pathogenesis of these metabolic disorders. Furthermore GPD2 links to proteins implicated in these diseases such as insulin receptor substrates and oxidative stress markers which further influence glucose and lipid homeostasis. Understanding GPD2's interaction with these elements helps in uncovering potential therapeutic targets for such diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Calcium-responsive mitochondrial glycerol-3-phosphate dehydrogenase which seems to be a key component of the pancreatic beta-cell glucose-sensing device.

See full target information GPD2

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제품이 사용된 논문 (15)

Recent publications for all applications. Explore the 전체 목록 and refine your search

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 38:e70195 PubMed39576231

2024

Rapid modulation of interscapular brown adipose tissue mitochondrial activity by ketosis induced by 1,3-butanediol administration to rats.

Applications

Unspecified application

Species

Unspecified reactive species

Paola Venditti,Gabriella Pinto,Vincenzo Migliaccio,Giuliana Panico,Gianluca Fasciolo,Rita De Matteis,Lillà Lionetti,Pieter de Lange,Stefania Serpico,Gaetana Napolitano,Claudio Agnisola,Angela Amoresano,Assunta Lombardi

PloS one 19:e0310530 PubMed39509399

2024

Isoscopoletin inhibits hepatocellular carcinoma cell proliferation via regulating glycolysis-related proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Chenyao Ruan,Chen Wang,Jiawen Gu,Zhihui Zhu

iScience 27:109796 PubMed38832016

2024

Mitochondrial glycerol 3-phosphate dehydrogenase deficiency exacerbates lipotoxic cardiomyopathy.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Qu,Xiufei Liu,Jiaran Zhu,Niexia He,Qingshan He,Linlin Zhang,Yuren Wang,Xiaoli Gong,Xin Xiong,Jinbo Liu,Chuan Wang,Gangyi Yang,Qingwu Yang,Gang Luo,Zhiming Zhu,Yi Zheng,Hongting Zheng

Cell death & disease 14:784 PubMed38036520

2023

The mitochondrial NADH shuttle system is a targetable vulnerability for Group 3 medulloblastoma in a hypoxic microenvironment.

Applications

Unspecified application

Species

Unspecified reactive species

J Contenti,Y Guo,A Mazzu,M Irondelle,M Rouleau,C Lago,G Leva,L Tiberi,I Ben-Sahra,F Bost,N M Mazure

The Journal of clinical investigation 133: PubMed36821389

2023

Kidney glycolysis serves as a mammalian phosphate sensor that maintains phosphate homeostasis.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Zhou,Petra Simic,Iris Y Zhou,Peter Caravan,Xavier Vela Parada,Donghai Wen,Onica L Washington,Maria Shvedova,Kerry A Pierce,Clary B Clish,Michael Mannstadt,Tatsuya Kobayashi,Marc N Wein,Harald Jüppner,Eugene P Rhee

Animals : an open access journal from MDPI 12: PubMed36496922

2022

Effect of miR-143-3p from Extracellular Vesicles of Porcine Uterine Luminal Fluid on Porcine Trophoblast Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yue Ding,Qun Hu,Jianyu Gan,Xupeng Zang,Ting Gu,Zhenfang Wu,Gengyuan Cai,Linjun Hong

Cell 185:4654-4673.e28 PubMed36334589

2022

Architecture of the outbred brown fat proteome defines regulators of metabolic physiology.

Applications

Unspecified application

Species

Unspecified reactive species

Haopeng Xiao,Luiz H M Bozi,Yizhi Sun,Christopher L Riley,Vivek M Philip,Mandy Chen,Jiaming Li,Tian Zhang,Evanna L Mills,Margo P Emont,Wenfei Sun,Anita Reddy,Ryan Garrity,Jiani Long,Tobias Becher,Laura Potano Vitas,Dina Laznik-Bogoslavski,Martha Ordonez,Xinyue Liu,Xiong Chen,Yun Wang,Weihai Liu,Nhien Tran,Yitong Liu,Yang Zhang,Aaron M Cypess,Andrew P White,Yuchen He,Rebecca Deng,Heiko Schöder,Joao A Paulo,Mark P Jedrychowski,Alexander S Banks,Yu-Hua Tseng,Paul Cohen,Linus T Tsai,Evan D Rosen,Samuel Klein,Maria Chondronikola,Fiona E McAllister,Nick Van Bruggen,Edward L Huttlin,Bruce M Spiegelman,Gary A Churchill,Steven P Gygi,Edward T Chouchani

Diabetes 70:1372-1387 PubMed33741719

2021

Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Qu,Xiaoli Gong,Xiufei Liu,Rui Zhang,Yuren Wang,Bangliang Huang,Linlin Zhang,Hongting Zheng,Yi Zheng

Oxidative medicine and cellular longevity 2020:8683404 PubMed32566106

2020

Improvement of Endothelial Dysfunction of Berberine in Atherosclerotic Mice and Mechanism Exploring through TMT-Based Proteomics.

Applications

Unspecified application

Species

Unspecified reactive species

Wangxiao Tan,Yu Wang,Kaiyue Wang,Siwei Wang,Jinghua Liu,Xiaoyan Qin,Yongna Dai,Xiaoying Wang,Xiumei Gao

International journal of molecular sciences 20: PubMed31618976

2019

The Potential of the FSP1cre- Mouse Model for Studying Juvenile NAFLD.

Applications

Unspecified application

Species

Unspecified reactive species

Jiapeng Chen,Yan Zhuang,Ming Keat Sng,Nguan Soon Tan,Walter Wahli

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GPD2 antibody [EPR14259]

Flow Cytometry (Intracellular) - Anti-GPD2 antibody [EPR14259] (AB188585)

Immunoprecipitation - Anti-GPD2 antibody [EPR14259] (AB188585)

Western blot - Anti-GPD2 antibody [EPR14259] (AB188585)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Reagents, Controls & Accessories

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (15)

Product promise