Anti-GPRC5A antibody [HL1864] - BSA and Azide free

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

ab325861 detects GPRC5A protein by immunohistochemical analysis. Sample : Paraffin-embedded human kidney. GPRC5A stained by ab325861 diluted at 1 : 100 and competitor's antibody diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

ab325861 detects GPRC5A protein by immunohistochemical analysis. Sample : Paraffin-embedded human lung cancer. GPRC5A stained by ab325861 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

ab325861 detects GPRC5A protein by immunohistochemical analysis. Sample : Paraffin-embedded human tissues. GPRC5A stained by ab325861 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

ab325861 detects GPRC5A protein by immunofluorescent analysis. Sample : Wild-type (WT) and GPRC5A knockout (KO) MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green : GPRC5A stained by ab325861 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

ab325861 detects GPRC5A protein by immunohistochemical analysis. Sample : Paraffin-embedded human tissues. GPRC5A stained by ab325861 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

ab325861 detects GPRC5A protein at cell membrane by immunofluorescent analysis. Sample : MCF-7 cells were fixed in ice-cold MeOH for 5 min. Green : GPRC5A stained by ab325861 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

• WB

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Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (AB325861)

Unboiled wild-type (WT) and GPRC5A knockout (KO) MCF-7 cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325861 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-GPRC5A antibody [HL1864] - BSA and Azide free (ab325861) at 1/1000 dilution

Lane 1:

Unboiled wild-type (WT) MCF-7 cell extracts at 30 µg

Lane 2:

Unboiled GPRC5A knockout (KO) MCF-7 cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false