AB226177

Anti-GRAF antibody

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Rabbit Polyclonal GRAF antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ARHGAP26 aa 600 to C-terminus.

대체 명칭 보기

GRAF, KIAA0621, OPHN1L, ARHGAP26, Rho GTPase-activating protein 26, GTPase regulator associated with focal adhesion kinase, Oligophrenin-1-like protein, Rho-type GTPase-activating protein 26, GRAF1

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Supplier Data

Immunoprecipitation - Anti-GRAF antibody (AB226177)

GRAF was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226177 at 6 μg per reaction. Western blot was performed from the immunoprecipitate using ab226177 at 0.4 μg/ml.

Lane 1 : ab226177 IP in Jurkat whole cell lysate.

Lane 2 : Control IgG IP in Jurkat whole cell lysate.

Detection : Chemiluminescence with exposure time of 3 minutes.

All lanes:

Immunoprecipitation - Anti-GRAF antibody (ab226177)

Predicted band size: 92 kDa

false

Supplier Data

Western blot - Anti-GRAF antibody (AB226177)

All lanes:

Western blot - Anti-GRAF antibody (ab226177) at 0.1 µg/mL

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Predicted band size: 92 kDa

true

Exposure time: 30s

주요 정보

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human ARHGAP26 aa 600 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UNA1

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cynomolgus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Immunogen

Purification 관련 사항

ab226177 was affinity purified using an epitope specific to GRAF immobilized on solid support.

보관 버퍼

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GRAF also known as GAP for Rho-associated domain containing protein F has a molecular mass of approximately 110 kDa. This protein functions mechanically as a GTPase-activating protein (GAP) that regulates Rho family GTPases which are essential in controlling cell shape and movement. GRAF expression is notable in several tissues including the colon brain and heart highlighting its involvement in various physiological processes.

Biological function summary

GRAF plays an important role in cytoskeletal dynamics impacting cell adhesion and motility. GRAF is part of a larger signaling complex that influences cellular morphology and signal transduction pathways. This interaction underpins its engagement in cellular activities associated with structural changes and response to external stimuli. Through its activity GRAF regulates the balance of actin polymerization and depolymerization essential for maintaining cellular integrity.

Pathways

GRAF's role intersects with the Rho signaling pathway which is critical for cell mobility and organization. This pathway contains proteins like RhoA and Rac1 where GRAF exerts its GTPase-activating function. GRAF also integrates into the integrin signaling cascade serving as a pivotal link between extracellular matrix interactions and intracellular cytoskeletal arrangements. These pathways establish GRAF's importance in coordinating cellular responses to environmental changes.

GRAF's involvement has been observed in certain cancers particularly colorectal cancer and cardiovascular diseases. In colorectal cancer altered GRAF expression associates with tumor progression and metastasis partly through its interaction with dysregulated RhoA signaling. In cardiovascular conditions changes in GRAF activity influence cardiac myocyte function and may contribute to heart failure. Understanding these connections helps in appreciating GRAF's potential as a therapeutic target in these diseases.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

GTPase-activating protein for RHOA and CDC42. Facilitates mitochondrial quality control by promoting Parkin-mediated recruitment of autophagosomes to damaged mitochondria (PubMed : 38081847). Negatively regulates the growth of human parainfluenza virus type 2 by inhibiting hPIV-2-mediated RHOA activation via interaction with two of its viral proteins P and V (PubMed : 27512058).. Isoform 2. Associates with MICAL1 on the endosomal membrane to promote Rab8-Rab10-dependent tubule extension. After dissociation of MICAL1, recruits WDR44 which connects the endoplasmic reticulum (ER) with the endosomal tubule, thereby participating in the export of a subset of neosynthesized proteins.

See full target information ARHGAP26

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제품이 사용된 논문 (1)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Cell death discovery 7:351 PubMed34772908

2021

DACH1 inhibits breast cancer cell invasion and metastasis by down-regulating the transcription of matrix metalloproteinase 9.

Applications

Unspecified application

Species

Unspecified reactive species

Sattout Aman,Yanan Li,Yunmeng Cheng,Yuxi Yang,Linlin Lv,Bowen Li,Kangkai Xia,Shujing Li,Huijian Wu

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GRAF antibody

Immunoprecipitation - Anti-GRAF antibody (AB226177)

Western blot - Anti-GRAF antibody (AB226177)

주요 정보

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Biochemicals

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

제품이 사용된 논문 (1)

Product promise