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AB242862

Anti-Progranulin antibody [EPR18539-58] - BSA and Azide free (Detector)

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Rabbit Recombinant Monoclonal Granulin antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
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Sandwich ELISA - Anti-Progranulin antibody [EPR18539-58] - BSA and Azide free (Detector) (AB242862)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Progranulin antibody [EPR18539-58] - BSA and Azide free (Detector) (AB242862)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab213473).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR18539-58

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB252364

Human Progranulin ELISA Kit

Recombinant|SimpleStep

Sandwich ELISA - Human Progranulin ELISA Kit (AB252364)

  • 0
  • 0
Sample Prep & Detection Kits

AB102918

PE / R-Phycoerythrin Conjugation Kit - Lightning-Link®

Flow Cytometry - PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (AB102918)

  • 5
  • 2
Sample Prep & Detection Kits

AB154873

Gold Conjugation Kit (40nm, 20 OD)

Conjugation - Gold Conjugation Kit (40nm, 20 OD) (AB154873)

  • 0
  • 0
Primary Antibodies

AB76541

Anti-CDX2 antibody [EPR2764Y]

RabMAb|Recombinant|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CDX2 antibody [EPR2764Y] (AB76541)

  • 4
  • 17
Primary Antibodies

AB4729

Anti-Histone H3 (acetyl K27) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (acetyl K27) antibody - ChIP Grade (AB4729)

  • 5
  • 85
Primary Antibodies

AB8898

Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade

BOND RX™ Validated

ChIP - Anti-Histone H3 (tri methyl K9) antibody - ChIP Grade (AB8898)

  • 5
  • 92
Primary Antibodies

AB1220

Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade

ChIP - Anti-Histone H3 (di methyl K9) antibody [mAbcam 1220] - ChIP Grade (AB1220)

  • 5
  • 72
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab242862 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Progranulin. The recommended pair for sandwich ELISA is:
Capture: ab242616, Mouse Progranulin Capture Antibody (unconjugated)
Detector: ab242862, Mouse Progranulin Detector Antibody (unconjugated)
The reference range value is 15.6 - 1000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Granulin also known as progranulin or GRN is a protein with a molecular mass of approximately 68 kDa. This protein is encoded by the GRN gene and is involved in various cellular processes. Granulin is expressed widely within the body particularly in epithelial cells immune cells and neurons. Researchers have developed methods like GRN ELISA and progranulin ELISA to quantitatively measure the levels of granulin in biological samples aiding in further understanding of its role in health and disease.
Biological function summary

Granulin functions as a growth factor involved in tissue repair cell proliferation and inflammation. It does not function as part of a larger protein complex but acts individually in exerting its effects on cells. Granulin influences various aspects of cellular architecture and signaling pathways making it important for maintaining cellular homeostasis. Additionally the granulin protein participates in regulating networks that involve cytokines and growth factors impacting both the innate and adaptive immune responses.

Pathways

Granulin plays significant roles in the MAPK and NF-kB signaling pathways. These pathways critically manage cellular reactions to stress inflammation and other external stimuli. Granulin modulates these processes often interacting with proteins like TNF receptors and interleukins to affect the downstream signaling events. Interactions between granulin and other signaling molecules allow it to integrate into broader networks that oversee various biological responses.

Significant links exist between granulin mutations and frontotemporal dementia (FTD) and Alzheimer's disease. Mutations in the GRN gene often lead to reduced granulin levels contributing to neurodegenerative conditions. These mutations affect the protein's normal function promoting pathological mechanisms associated with neuronal deterioration. Granulin interacts with proteins such as TDP-43 in neurodegenerative diseases where alterations might result in protein aggregation and neurotoxicity. Understanding these connections can lead to potential therapeutic interventions targeting granulin-related pathways.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Secreted protein that acts as a key regulator of lysosomal function and as a growth factor involved in inflammation, wound healing and cell proliferation (PubMed : 12524533, PubMed : 20026663, PubMed : 23041626, PubMed : 27789271, PubMed : 28073925, PubMed : 28453791, PubMed : 28541286, PubMed : 8496151). Regulates protein trafficking to lysosomes and, also the activity of lysosomal enzymes (PubMed : 27789271, PubMed : 28453791, PubMed : 28541286). Facilitates also the acidification of lysosomes, causing degradation of mature CTSD by CTSB (PubMed : 28073925). In addition, functions as a wound-related growth factor that acts directly on dermal fibroblasts and endothelial cells to promote division, migration and the formation of capillary-like tubule structures (PubMed : 12524533). Also promotes epithelial cell proliferation by blocking TNF-mediated neutrophil activation preventing release of oxidants and proteases (PubMed : 8496151). Moreover, modulates inflammation in neurons by preserving neurons survival, axonal outgrowth and neuronal integrity (PubMed : 20026663, PubMed : 23041626).. Granulin-3. Inhibits epithelial cell proliferation and induces epithelial cells to secrete IL-8.. Granulin-7. Stabilizes CTSD through interaction with CTSD leading to maintain its aspartic-type peptidase activity.
See full target information Grn

대체 명칭 보기

Progranulin, PGRN, Acrogranin, Epithelin/granulin precursor, Glycoprotein of 88 Kda, PC cell-derived growth factor, Proepithelin, GP88, Glycoprotein 88, PCDGF, PEPI, Grn
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Product promise

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