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AB244799

Anti-Granzyme B antibody [EPR20130-11] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal Granzyme B antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
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Sandwich ELISA - Anti-Granzyme B antibody [EPR20130-11] - BSA and Azide free (Capture) (AB244799)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-Granzyme B antibody [EPR20130-11] - BSA and Azide free (Capture) (AB244799)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab238265).

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR20130-11

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Assay Kits

AB206386

TUNEL Assay Kit - HRP-DAB

Immunohistochemistry - TUNEL Assay Kit - HRP-DAB (AB206386)

  • 4
  • 3
Sample Prep & Detection Kits

AB269822

Alexa Fluor® 594 Conjugation Kit (Fast) - Lightning-Link®

Schematic Diagram - Alexa Fluor® 594 Conjugation Kit (Fast) - Lightning-Link® (AB269822)

  • 5
  • 1
Sample Prep & Detection Kits

AB269894

Latex Conjugation Kit - 400nm Black

Functional Studies - Latex Conjugation Kit - 400nm Black (AB269894)

  • 0
  • 0
Primary Antibodies

AB313711

Anti-Granzyme K antibody [EPR24601-14] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Granzyme K antibody [EPR24601-14] - BSA and Azide free (Detector) (AB313711)

  • 0
  • 0
Primary Antibodies

AB259680

Anti-Granzyme A antibody [EPR22160-10] - BSA and Azide free (Detector)

RabMAb|Recombinant

Sandwich ELISA - Anti-Granzyme A antibody [EPR22160-10] - BSA and Azide free (Detector) (AB259680)

  • 0
  • 0
Assay Kits

AB244123

Human Granzyme B Antibody Pair - BSA and Azide free

RabMAb|Recombinant

Sandwich ELISA - Human Granzyme B Antibody Pair - BSA and Azide free (AB244123)

  • 0
  • 0
Primary Antibodies

AB237365

Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Alexa Fluor® 647 Anti-CD8 alpha antibody [EPR21769] (AB237365)

  • 0
  • 0
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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제품 세부 정보

ab244799 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse Granzyme B. The recommended pair for sandwich ELISA is:
Capture: ab244799, Mouse Granzyme B Capture Antibody (unconjugated)
Detector: ab245072, Mouse Granzyme B Detector Antibody (unconjugated)
The reference range value is 188 - 12000 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme B also known as GZMB GB11 or granzyme B protein is a serine protease with a molecular mass of approximately 32 kDa. It is expressed mainly in cytotoxic T lymphocytes and natural killer (NK) cells. This enzyme plays a mechanical role in inducing apoptosis in target cells serving as an effector protein in the immune system's defense against virally infected cells or transformed cancer cells. The activity of granzyme B relies on its ability to cleave after aspartate residues in substrate proteins leading to the activation of apoptotic pathways.
Biological function summary

Granzyme B participates prominently in the immune response by activating caspases particularly caspase-3 which promotes the breakdown of cellular components necessary for apoptosis. Granzyme B does not function in isolation but acts in concert with other immune system factors such as perforin to effectively induce cell death. Perforin creates pores in the target cell membrane allowing granzyme B to enter and instigate the apoptosis sequence. The enzyme also contributes to the processing of cytokines which enhances the immune response further.

Pathways

Studies have determined that granzyme B is critical in the apoptosis pathway particularly in the granule exocytosis pathway. It closely interacts with proteins such as perforin and other granzymes to mediate apoptosis in target cells. Granzyme B also plays a role in the inflammatory response and can influence pathways associated with cytotoxic T cell signaling. Its pathway interactions ensure effective elimination of damaged or infected cells maintaining tissue homeostasis.

Granzyme B has associations with autoimmune diseases and cancer. Abnormally high levels of granzyme B can contribute to tissue damage and inflammation in autoimmune conditions like rheumatoid arthritis. In the context of cancer granzyme B aids in tumor surveillance and destruction when functioning correctly but impaired granzyme B activity can lead to evasion of immune detection by cancerous cells. Perforin also plays a role in these conditions closely working with granzyme B to either protect against or drive disease progression.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse (PubMed : 3555842, PubMed : 35705808). It cleaves after Asp (PubMed : 35705808). Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death (By similarity). Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution (By similarity). Cleaves caspase-3 and -9 (CASP3 and CASP9, respectively) to give rise to active enzymes mediating apoptosis (PubMed : 35705808). Cleaves and activates CASP7 in response to bacterial infection, promoting plasma membrane repair (PubMed : 35705808).
See full target information Gzmb

대체 명칭 보기

Ctla-1, Ctla1, Gzmb, CTLA-1, Cytotoxic cell protease 1, Fragmentin-2, CCP1
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Product promise

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