AB282703

Anti-Granzyme K antibody [EPR24601-164]

Anti-Granzyme K 항체 [EPR24601-164]

BOND RX™ Validated
20ul selling size
RabMAb
Recombinant
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(4 제품이 사용된 논문 )

Anti-Granzyme K antibody [EPR24601-164] (ab282703) is a rabbit monoclonal antibody detecting Granzyme K in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

7 이미지

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labelling Granzyme K with ab282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human tonsil. (PMID : 22207739). The section was incubated with ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma tissue labelling Granzyme K with ab282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on immune cells of human cervical carcinoma. The section was incubated with ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemical analysis of paraffin-embedded human diffuse large B-cell lymphoma tissue labelling Granzyme K with ab282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used. Positive staining on human diffuse large B-cell lymphoma. The section was incubated with ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemical analysis of paraffin-embedded human NKT lymphoma tissue labelling Granzyme K with ab282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on human NKT lymphoma. The section was incubated with ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemical analysis of paraffin-embedded human pancreas tissue labelling Granzyme K with ab282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control : no staining on human pancreas. The section was incubated with ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemical analysis of paraffin-embedded human endometrium tissue labelling Granzyme K with 282703 at 1/1000 dilution (0.485 ug/ml), followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection). Positive staining on lymphocytes in human endometrium. The section was incubated with ab282703 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection) was used.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

Lab

Western blot - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Blocking and diluting buffer and concentration : 5% NFDM/TBST

The expression profile/molecular weight observed is consistent with what has been described in the literature (PMID : 10407175, 18559365).

Negative control : heart (PMID : 10407175).

Exposure time : 15 seconds

All lanes:

Western blot - Anti-Granzyme K antibody [EPR24601-164] (ab282703) at 1/1000 dilution

Lane 1:

Human spleen tissue lysate at 40 µg

Lane 2:

Human tonsil tissue lysate at 40 µg

Lane 3:

Human lymphoma tissue lysate at 40 µg

Lane 4:

Human heart tissue lysate at 40 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/ko/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/2000 dilution

Predicted band size: 29 kDa

Observed band size: 22 kDa,27 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24601-164

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/1000", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

제품 세부 정보

What is this antibody validated in?
Anti-Granzyme K antibody [EPR24601-164] (ab282703) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Granzyme K?
Anti-Granzyme K [EPR24601-164] (ab282703) specifically detects a band for Granzyme K (UniProt: P49863) at a molecular weight of 29kDa.

Other related products
We have a range of other formats of antibody clone [EPR24601-164] also available for your convenience: ab282703, Carrier free - ab282714

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 20µl. Discover our selection of trial-size antibodies.

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Granzyme K also known as protein K or 24c3 is a serine protease with a mass of approximately 27 kilodaltons. It belongs to the granzyme family of proteins which are mainly found within the cytotoxic granules of natural killer (NK) cells and cytotoxic T lymphocytes (CTLs). These immune cells store granzyme K in their granules which they release to induce apoptosis in target cells. Researchers have identified granzyme K expression in various tissues and immune cells highlighting its widespread role in modulating immune responses.

Biological function summary

Granzyme K serves as a potent mediator in the immune system by inducing apoptosis in target cells. It does not act alone but rather in concert with other granzymes and proteins to effectively execute cell death. Granzyme K can degrade cellular proteins once introduced into a target cell's cytoplasm leading to apoptosis. The protease functions independently and does not require a complex for its activity contributing to the immune system's ability to eliminate infected or transformed cells.

Pathways

The granzyme K protein exists within essential immune regulatory pathways such as the cytotoxic T lymphocyte-induced apoptosis pathway and the natural killer cell-mediated cytotoxicity pathway. Granzyme K acts alongside granzymes A and B to promote programmed cell death and finely tune host immune responses. While granzymes A and B have more defined roles granzyme K complements their activity offering redundancy and ensuring efficient elimination of harmful target cells.

Granzyme K shows significant connections to inflammatory conditions and autoimmune diseases such as rheumatoid arthritis. Elevated granzyme K levels in these diseases may result from dysregulated immune responses leading to excessive tissue damage. In autoimmune disorders granzyme K works in combination with proteins like perforin which facilitates entry of granzymes into the cell to stimulate apoptosis. Understanding granzyme K's role in these conditions may help develop therapeutic approaches to modulate immune activity and minimize tissue damage.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Serine protease that initiates the GZMK pathway of the complement system, a cascade of proteins directly activated by CD8(+) T-cells that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system (PubMed : 3262682, PubMed : 39814882, PubMed : 39914456). GZMK is specifically secreted by CD8(+) T-cells and mediates both recognition and initiation steps of GZMK complement pathway (PubMed : 39914456). First acts as a pattern recognition receptor, which specifically recognizes and binds heparan sulfate glycosaminoglycans on the pathogen surface to drive opsonization (PubMed : 39914456). It then initiates the complement pathway cascade by catalyzing cleavage and activation of C2 and C4, the next components of the complement pathway (PubMed : 39814882, PubMed : 39914456). GZMK-mediated complement activation is an important contributor to tissue inflammation (PubMed : 24711407, PubMed : 33271118, PubMed : 35704599, PubMed : 39614076, PubMed : 39814882, PubMed : 39914456). Also able to cleave and activate F2R/PAR1 and PAR2/F2RL1, possibly promoting interleukin release (PubMed : 21760880, PubMed : 26936634, PubMed : 35881628). May also regulate apoptosis by catalyzing cleavage and activation of BID, thereby promoting cytochrome C release from mitochrondria (PubMed : 17308307).

See full target information GZMK

대체 명칭 보기

TRYP2, GZMK, Granzyme K, GrK, Fragmentin-3, Granzyme-3, NK-tryptase-2, NK-Tryp-2

style

left-column

제품이 사용된 논문 (4)

Recent publications for all applications. Explore the 전체 목록and refine your search

World journal of diabetes 16:109553 PubMed40980297

2025

Farrerol ameliorates diabetic cardiomyopathy by inhibiting ferroptosis miR-29b-3p/SIRT1 signaling pathway in endothelial cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Guo,Xin-Rou Yu,Hao-Di Gu,Yu-Jie Wang,Zhen-Gang Yang,Ju-Fang Chi,Liu-Ping Zhang,Hui Lin

Cellular and molecular gastroenterology and hepatology 19:101411 PubMed39349248

2024

Single-cell Profiling of Intrahepatic Immune Cells Reveals an Expansion of Tissue-resident Cytotoxic CD4 T Lymphocyte Subset Associated With Pathogenesis of Alcoholic-associated Liver Diseases.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Gao,Shiguan Wang,Xiaoyu Xie,Pierluigi Ramadori,Xinying Li,Xiaoyu Liu,Xue Ding,Jinyuan Liang,Bowen Xu,Yawei Feng,Xueying Tan,Haoran Wang,Yan Zhang,Haiyan Zhang,Tingguo Zhang,Ping Mi,Shiyang Li,Cuijuan Zhang,Detian Yuan,Mathias Heikenwalder,Peng Zhang

NPJ precision oncology 8:159 PubMed39060379

2024

Novel molecular subtypes of METex14 non-small cell lung cancer with distinct biological and clinical significance.

Applications

Unspecified application

Species

Unspecified reactive species

Shengnan Chen,Tao Hu,Jikai Zhao,Qian Zhu,Jin Wang,Zhan Huang,Chan Xiang,Ruiying Zhao,Changbin Zhu,Shun Lu,Yuchen Han

Environmental toxicology 39:890-904 PubMed37956258

2023

Multidimensional progressive single-cell sequencing reveals cell microenvironment composition and cancer heterogeneity in lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Chen,Anqi Song,Faisal Ul Rehman,Dan Han

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Granzyme K antibody [EPR24601-164]

Anti-Granzyme K 항체 [EPR24601-164]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

Western blot - Anti-Granzyme K antibody [EPR24601-164] (AB282703)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (4)

Product promise