Anti-Gremlin 1 antibody [EPR29485-567]

• ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-Gremlin 1 antibody [EPR29485-567] (AB326998)

Immunofluorescent analysis of 100% methanol-fixed, 0.1% TritonX-100 permeabilized MRC-5 (human lung fibroblast) and MCF7 (human breast adenocarcinoma epithelial cell) cells labelling Gremlin 1 with ab326998 at 1/50 (9.86 μg/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 dilution (Green).

Confocal image showing cytoplasmic staining in MRC-5 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).

Low expression : MCF7

Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 dilution, followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1 : 1000 dilution (Magenta).

• I-ELISA

Lab

Indirect ELISA - Anti-Gremlin 1 antibody [EPR29485-567] (AB326998)

Indirect ELISA analysis of ab326998 at 1000-0 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1 : 2500 dilution dilution.

Antigen : Human full-length Gremlin 1;Human full-length Gremlin 2.

Antigen concentration : 1000 ng/ml

This antibody does not cross-react with recombinant human Gremlin 2 by ELISA.

• WB

Lab

Western blot - Anti-Gremlin 1 antibody [EPR29485-567] (AB326998)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : MCF7, T-47D.

The band at approximately 50 kDa is of unknown identity.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-Gremlin 1 antibody [EPR29485-567] (ab326998) at 1/1000 dilution

Lane 1:

IMR-90 (human lung fibroblast) whole cell lysate at 20 µg

Lane 2:

MRC-5 (human lung fibroblast) whole cell lysate at 20 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

Lane 5:

MG-63 (human osteosarcoma fibroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/ko/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 26-28 kDa,36 kDa

false

Exposure time: 180s