AB233734

Anti-GRIK3/GluK3 antibody [2B3D1]

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(2 제품이 사용된 논문 )

Mouse Monoclonal GRIK3/GluK3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human GRIK3 aa 1-200.

3 이미지

Immunocytochemistry/ Immunofluorescence - Anti-GRIK3/GluK3 antibody [2B3D1] (AB233734)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRIK3/GluK3 antibody [2B3D1] (AB233734)

Immunofluorescence analysis of Hela (human epithelial cell line from cervix adenocarcinoma) cells labelling GRIK3/GluK3 using ab233734 at 1/200 dilution. Blue : DRAQ5 fluorescent DNA dye. Red : Actin filaments labeled with AlexaFluor^®-555-Phalloidin.

Supplier Data

Western blot - Anti-GRIK3/GluK3 antibody [2B3D1] (AB233734)

All lanes:

Western blot - Anti-GRIK3/GluK3 antibody [2B3D1] (ab233734) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

GRIK3/GluK3 (aa 32-173)-hIgGFc transfected HEK293 cell lysate

Predicted band size: 104 kDa

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Supplier Data

Western blot - Anti-GRIK3/GluK3 antibody [2B3D1] (AB233734)

Expected MW is 42 kDa.

All lanes:

Western blot - Anti-GRIK3/GluK3 antibody [2B3D1] (ab233734) at 1/500 dilution

All lanes:

Human GRIK3/GluK3 (aa 32-173) recombinant protein

Predicted band size: 104 kDa

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주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

2B3D1

Isotype

IgG1

Carrier free

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human GRIK3 aa 1-200. The exact immunogen used to generate this antibody is proprietary information.

Q13003

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cynomolgus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein G

Purification 관련 사항

Purified from tissue culture supernatant.

보관 버퍼

Preservative: 0.05% Sodium azide Constituents: PBS

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The GRIK3 gene encodes the protein GluK3 also known as kainate receptor subunit 3. This protein is involved in mediating synaptic transmission in the central nervous system. GluK3 is part of the ionotropic glutamate receptor family which forms ligand-gated ion channels. The protein has a molecular mass of approximately 107 kDa. GRIK3 is expressed in several regions of the brain including the cortex hippocampus and cerebellum indicating its important roles in these areas.

Biological function summary

The ionotropic receptor family includes GluK3 which forms a functional protein complex known for mediating excitatory neurotransmission. The activity of GluK3 involves the binding of glutamate leading to the opening of cation channels and allowing the flow of sodium and potassium ions. This process results in the depolarization of neurons contributing to synaptic plasticity and excitatory synaptic activity in the brain. The precise functioning of GluK3 is important for maintaining normal synaptic transmission and neuronal communication.

Pathways

The GRIK3/GluK3 protein significantly contributes to the glutamatergic synapse pathway and influences the long-term potentiation (LTP) pathway. GluK3 plays a role in regulating synaptic strength and plasticity by modulating neurotransmitter release and postsynaptic responses. Throughout these pathways it interacts with proteins such as GRIA1 and GRIN2 which are other members of the glutamate receptor family. These interactions ensure proper signal transmission and synaptic adaptation throughout neural circuits.

GRIK3/GluK3 shows associations with psychiatric conditions such as schizophrenia and bipolar disorder. The dysregulation of GluK3 function can lead to aberrant glutamatergic signaling which is implicated in the pathophysiology of these mental health disorders. Furthermore altered GluK3 activity may impact proteins like GRIA2 affecting the overall balance of excitatory and inhibitory signaling in the brain. Understanding the role of GRIK3 in these diseases may help in developing therapeutic interventions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Ionotropic glutamate receptor that functions as a cation-permeable ligand-gated ion channel, gated by L-glutamate and the glutamatergic agonist kainic acid. Binding of the excitatory neurotransmitter L-glutamate induces a conformation change, leading to the opening of the cation channel, and thereby converts the chemical signal to an electrical impulse. The receptor then desensitizes rapidly and enters a transient inactive state, characterized by the presence of bound agonist (PubMed : 7719709). In association with GRIK2, involved in presynaptic facilitation of glutamate release at hippocampal mossy fiber synapses (By similarity).

See full target information GRIK3

대체 명칭 보기

GLUR7, GRIK3, GluK3, Excitatory amino acid receptor 5, Glutamate receptor 7, EAA5, GluR-7, GluR7

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제품이 사용된 논문 (2)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Journal of cellular physiology 239:e31062 PubMed37357387

2023

Periodontal ligament-associated protein-1 knockout mice regulate the differentiation of osteoclasts and osteoblasts through TGF-β1/Smad signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Shuang Liu,Xiao Yan,Jing Guo,Hong An,Xingrui Li,Liying Yang,Xijiao Yu,Shu Li

American journal of cancer research 13:2066-2075 PubMed37293152

2023

GRIK3 deficiency promotes non-small cell lung cancer progression by the regulation of the UBE2C/CDK1/Wnt signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Liu,Zhu-Xiang Zhao,Bin-Kai Li,Zi-Wen Zhao

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GRIK3/GluK3 antibody [2B3D1]

Immunocytochemistry/ Immunofluorescence - Anti-GRIK3/GluK3 antibody [2B3D1] (AB233734)

Western blot - Anti-GRIK3/GluK3 antibody [2B3D1] (AB233734)

Western blot - Anti-GRIK3/GluK3 antibody [2B3D1] (AB233734)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

Purification 관련 사항

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (2)

Product promise