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AB247671

Anti-GRK1 antibody [EPR2039(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal GRK1 antibody. Carrier free. Suitable for WB and reacts with Human samples.

대체 명칭 보기

RHOK, GRK1, Rhodopsin kinase GRK1, RK, G protein-coupled receptor kinase 1

1 이미지
Western blot - Anti-GRK1 antibody [EPR2039(2)] - BSA and Azide free (AB247671)
  • WB

Unknown

Western blot - Anti-GRK1 antibody [EPR2039(2)] - BSA and Azide free (AB247671)

This data was developed using ab108502, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-GRK1 antibody [EPR2039(2)] - BSA and Azide free (ab247671) at 1/1000 dilution

Lane 1:

Y79 cell lysate at 10 µg

Lane 2:

JAR cell lysate at 10 µg

Predicted band size: 63 kDa

Observed band size: 60 kDa

false

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2039(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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보완 제품

Primary Antibodies

AB109303

Anti-GRK3 antibody [EPR2041(2)]

RabMAb|Recombinant|Trial Size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK3 antibody [EPR2041(2)] (AB109303)

  • 0
  • 0
Primary Antibodies

AB244364

Anti-GRK6 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK6 antibody (AB244364)

  • 0
  • 0
Primary Antibodies

AB64943

Anti-GRK5 antibody

BOND RX™ Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GRK5 antibody (AB64943)

  • 5
  • 3
Primary Antibodies

AB124964

Anti-alpha smooth muscle Actin antibody [EPR5368]

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [EPR5368] (AB124964)

  • 5
  • 19
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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제품 세부 정보

ab247671 is the carrier-free version of ab108502.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.2 - 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
+4°C
보관 정보
Do Not Freeze
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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

GRK1 also known as G-protein-coupled receptor kinase 1 or rhodopsin kinase is an enzyme with a molecular mass of approximately 63 kDa. It plays a critical role in the phosphorylation process of activated rhodopsin. This kinase is mainly expressed in the retina particularly in rod photoreceptor cells where it aids in the regulation of phototransduction by rapidly shutting off the rhodopsin signaling cycle.
Biological function summary

GRK1 functions as part of a coordinated mechanism that ensures proper desensitization and recovery of photoreceptors after they are exposed to light. It is instrumental in the phototransduction cascade preventing excessive activation by phosphorylating light-activated rhodopsin which then allows the binding of arrestin. This phosphorylation-arrestin binding step is part of a larger complex that maintains the sensitivity of photoreceptors to changing light conditions.

Pathways

GRK1 participates in the critical visual signal transduction pathway within the retina. It works in conjunction with proteins such as arrestin and rhodopsin to modulate the phototransduction cascade ensuring proper visual responses. This kinase interacts with these proteins to modulate their activity and help in adapting to varying light conditions emphasizing its importance in maintaining visual clarity.

GRK1 mutations are connected to conditions like Oguchi disease a type of congenital stationary night blindness. This disease arises when rhodopsin phosphorylation is disrupted leading to impaired vision adaptation in low light. GRK1 links to the arrestin protein in this context as both proteins are necessary for proper rod cell function and dysfunction in either can lead to similar visual disorders.
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

Retina-specific kinase involved in the signal turnoff via phosphorylation of rhodopsin (RHO), the G protein- coupled receptor that initiates the phototransduction cascade (PubMed : 15946941). This rapid desensitization is essential for scotopic vision and permits rapid adaptation to changes in illumination (By similarity). May play a role in the maintenance of the outer nuclear layer in the retina (By similarity).
See full target information GRK1
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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