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AB325867

Anti-GRM5 antibody [HL2876] - BSA and Azide free

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Rabbit Recombinant Monoclonal Metabotropic Glutamate Receptor 5 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Rat, Mouse, Human samples. Immunogen corresponding to Synthetic Peptide within Human GRM5.
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Immunocytochemistry/ Immunofluorescence - Anti-GRM5 antibody [HL2876] - BSA and Azide free (AB325867)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-GRM5 antibody [HL2876] - BSA and Azide free (AB325867)

ab325867 detects mGluR5 protein by immunofluorescent analysis. Sample : DIV11 rat E18 hippocampal neurons and glial cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : mGluR5 stained by ab325867 diluted at 1 : 250. Red : Tau, an axon marker, stained by Tau antibody diluted at 1 : 500. Blue : Fluoroshield with DAPI.

Western blot - Anti-GRM5 antibody [HL2876] - BSA and Azide free (AB325867)
  • WB

Supplier Data

Western blot - Anti-GRM5 antibody [HL2876] - BSA and Azide free (AB325867)

Rat tissue extract (50 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with ab325867 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-GRM5 antibody [HL2876] - BSA and Azide free (ab325867) at 1/1000 dilution

All lanes:

Rat brain tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

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주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL2876

Isotype

IgG

Carrier free

Yes

Reacts with

Rat, Mouse, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human GRM5.

P41594

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보완 제품

Primary Antibodies

AB115777

Anti-beta Actin antibody [SP124] - Cytoskeleton Marker

20ul selling size|RabMAb|Lab Essentials|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody [SP124] - Cytoskeleton Marker (AB115777)

  • 4
  • 1
Primary Antibodies

AB259335

Anti-FMRP antibody [EPR23852-90]

BOND RX™ Validated|20ul selling size|Recombinant|Advanced Validation|RabMAb

Immunoprecipitation - Anti-FMRP antibody [EPR23852-90] (AB259335)

  • 0
  • 0
Primary Antibodies

AB181602

Anti-GAPDH antibody [EPR16891] - Loading Control

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GAPDH antibody [EPR16891] - Loading Control (AB181602)

  • 5
  • 23
Primary Antibodies

AB82211

Anti-mGluR1 antibody

Immunocytochemistry/ Immunofluorescence - Anti-mGluR1 antibody (AB82211)

  • 5
  • 15
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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" }, "Rat": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>" } } }
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특성 및 보관 정보

제형
Liquid
Purification 테크닉
Affinity purification Protein A
보관 버퍼
pH: 7.4 Constituents: PBS
배송 시 보관 조건
Blue Ice
적절한 단기 보관 기간
1-2 weeks
적절한 단기 보관 조건
+4°C
적절한 장기 보관 조건
-20°C
분주 정보
Upon delivery aliquot
보관 정보
Avoid freeze / thaw cycle
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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:
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타겟 정보

G-protein coupled receptor for glutamate. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors. Signaling activates a phosphatidylinositol-calcium second messenger system and generates a calcium-activated chloride current. Plays an important role in the regulation of synaptic plasticity and the modulation of the neural network activity.
See full target information GRM5

대체 명칭 보기

GPRC1E, MGLUR5, GRM5, Metabotropic glutamate receptor 5, mGluR5
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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.
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