AB171089

Anti-Grp75/MOT antibody [9F8]

KD Validated
각 태그의 뜻

Be the first to review this product! 리뷰 제출

(6 제품이 사용된 논문 )

Mouse Monoclonal Grp75/MOT antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Rat, African green monkey samples. Cited in 6 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human HSPA9.

5 이미지

Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (AB171089)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (AB171089)

Immunofluorescence analysis of Grp75/MOT was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ab171089 at 1 : 50 dilution in 0.1% BSA, incubated at 4°C overnight and then labeled with Goat anti-Mouse IgG (H+L) Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1 : 2000 for 45 minutes at room temperature (Panel a : green). Nuclei (Panel b : blue) were stained with DAPI. F-actin (Panel c : red) was stained with Rhodamine Phalloidin. Panel d represents the merged image showing mitochondrial localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (AB171089)

Immunofluorescent analysis of HeLa cells labeling Grp75/MOT with ab171089 at 1/50 dilution (green) compared to negative control NIH3T3 cells.

Supplier Data

Immunoprecipitation - Anti-Grp75/MOT antibody [9F8] (AB171089)

Detection of Grp75/MOT in Immunoprecipitates of HeLa whole cell lysates using ab171089 at 2μg per 750μg lysate. Detected with ab171089 at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-Grp75/MOT antibody [9F8] (ab171089)

Predicted band size: 74 kDa

false

Supplier Data

Western blot - Anti-Grp75/MOT antibody [9F8] (AB171089)

All lanes:

Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089) at 1/1000 dilution

Lane 1:

Untransfected HeLa cells

Lane 2:

HeLa cells transfected with scrambled siRNA

Lane 3:

HeLa cells transfected with Grp75/MOT siRNA

Secondary

All lanes:

Goat anti-Mouse IgG (H+L) HRP conjugate at 1/4000 dilution

Predicted band size: 74 kDa

false

Supplier Data

Western blot - Anti-Grp75/MOT antibody [9F8] (AB171089)

4-20% Tris-HCL polyacrylamide gel

All lanes:

Western blot - Anti-Grp75/MOT antibody [9F8] (ab171089) at 1/1000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 50 µg

Lane 3:

K-562 (Human chronic myelogenous leukemia lymphoblast cell line ) whole cell lysate at 50 µg

Lane 4:

A-431 (Human epidermoid carcinoma cell line) whole cell lysate at 50 µg

Lane 5:

COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate at 50 µg

Lane 6:

NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate at 50 µg

Lane 7:

NRK (Rat kidney cell line) whole cell lysate at 50 µg

Secondary

All lanes:

Goat anti-Mouse HRP at 1/20000 dilution

Predicted band size: 74 kDa

true

주요 정보

Host species

Mouse

Clonality

Monoclonal

Clone number

9F8

Isotype

IgG

Carrier free

Reacts with

Rat, Human, African green monkey

Applications

ICC/IF, IP, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human HSPA9.

P38646

style

left-column

style

left-column

Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "Use 2μg.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

style

left-column

특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

Preservative: 0.05% Sodium azide Constituents: PBS, 30% Glycerol (glycerin, glycerine), 0.1% BSA

배송 시 보관 조건

Blue Ice

적절한 단기 보관 기간

1-2 weeks

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

분주 정보

Upon delivery aliquot

보관 정보

Avoid freeze / thaw cycle

style

left-column

추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

Grp75 also known as MOT is a chaperone protein with a molecular mass of approximately 75 kDa. This protein is part of the heat shock protein 70 family and often referred to as Grp75 protein or MOT protein. Typically Grp75 is expressed in the mitochondria of eukaryotic cells. Its primary function lies in aiding the folding and assembly of newly synthesized proteins and responding to cellular stress conditions. The MOT-238 and MOT proteins are related variants in this protein family and antibodies like anti-MOT can specifically detect Grp75.

Biological function summary

Grp75 facilitates important cellular processes through its involvement in the regulation of mitochondrial homeostasis and cellular stress responses. Grp75 forms complexes with other mitochondrial proteins ensuring proper function of protein import and maintenance of mitochondrial DNA. It plays an essential role in the cellular response to stress by interacting with other heat shock proteins such as Hsp60 and by stabilizing proteins under conditions of thermal and oxidative stress. Additionally Grp75 is connected to the regulation of cell proliferation and apoptosis mechanisms integral to maintaining cellular balance.

Pathways

Grp75 is heavily involved in the mitochondrial unfolded protein response and apoptosis pathways. It associates with proteins like PINK1 and Parkin which are vital in the selective autophagy of mitochondria known as mitophagy. In response to misfolded proteins Grp75 helps to maintain mitochondrial function and promotes cell survival through interaction with other mitochondrial proteins. The JG1 and 9F8 proteins are also part of these pathways linked through their roles in protein quality control mechanisms within the cell.

Grp75 has been implicated in neurodegenerative diseases such as Parkinson's disease and certain cancers. Mutations or functional deficiencies in Grp75 can disrupt mitochondrial function which is a critical factor in the pathogenesis of Parkinson’s disease. The connection to proteins like PINK1 and Parkin in this context highlights the role Grp75 plays in the etiology of the disorder. In cancer Grp75 contributes to the cellular stress response and its overexpression can lead to drug resistance making it a target of interest for developing therapeutic strategies.

style

left-column

style

left-column

제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

타겟 정보

Mitochondrial chaperone that plays a key role in mitochondrial protein import, folding, and assembly. Plays an essential role in the protein quality control system, the correct folding of proteins, the re-folding of misfolded proteins, and the targeting of proteins for subsequent degradation. These processes are achieved through cycles of ATP binding, ATP hydrolysis, and ADP release, mediated by co-chaperones (PubMed : 18632665, PubMed : 25615450, PubMed : 28848044, PubMed : 30933555, PubMed : 31177526). In mitochondria, it associates with the TIM (translocase of the inner membrane) protein complex to assist in the import and folding of mitochondrial proteins (By similarity). Plays an important role in mitochondrial iron-sulfur cluster (ISC) biogenesis, interacts with and stabilizes ISC cluster assembly proteins FXN, NFU1, NFS1 and ISCU (PubMed : 26702583). Regulates erythropoiesis via stabilization of ISC assembly (PubMed : 21123823, PubMed : 26702583). Regulates mitochondrial calcium-dependent apoptosis by coupling two calcium channels, ITPR1 and VDAC1, at the mitochondria-associated endoplasmic reticulum (ER) membrane to facilitate calcium transport from the ER lumen to the mitochondria intermembrane space, providing calcium for the downstream calcium channel MCU, which releases it into the mitochondrial matrix (By similarity). Although primarily located in the mitochondria, it is also found in other cellular compartments. In the cytosol, it associates with proteins involved in signaling, apoptosis, or senescence. It may play a role in cell cycle regulation via its interaction with and promotion of degradation of TP53 (PubMed : 24625977, PubMed : 26634371). May play a role in the control of cell proliferation and cellular aging (By similarity). Protects against reactive oxygen species (ROS) (By similarity). Extracellular HSPA9 plays a cytoprotective role by preventing cell lysis following immune attack by the membrane attack complex by disrupting formation of the complex (PubMed : 16091382).

See full target information HSPA9

대체 명칭 보기

GRP75, HSPA9B, mt-HSP70, HSPA9, 75 kDa glucose-regulated protein, Heat shock 70 kDa protein 9, Heat shock protein family A member 9, Mortalin, Peptide-binding protein 74, GRP-75, MOT, PBP74

style

left-column

제품이 사용된 논문 (6)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Aging 16:11062-11071 PubMed38975935

2024

Sevoflurane impedes neuropathic pain by maintaining endoplasmic reticulum stress and oxidative stress homeostasis through inhibiting the activation of the PLCγ/CaMKII/IP3R signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

An Xie,Xianjie Zhang,Feng Ju,Yukai Zhou,Dan Wu,Jia Han

Frontiers in aging neuroscience 14:990679 PubMed36337694

2022

Sevoflurane exposure causes neuronal apoptosis and cognitive dysfunction by inducing ER stress activation of the inositol 1, 4, 5-trisphosphate receptor.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Zhang,Yanan Li,Xupeng Wang,Chunping Yin,Qi Zhou,Junfei Guo,Juan Zhao,Xiaohui Xian,Zhiyong Hou,Qiujun Wang

Cell death & disease 12:701 PubMed34262025

2021

ATF5, a putative therapeutic target for the mitochondrial DNA 3243A > G mutation-related disease.

Applications

Unspecified application

Species

Unspecified reactive species

Xinpei Gao,Zhixin Jiang,Xinfeng Yan,Jiping Liu,Fengwen Li,Peng Liu,Jialu Li,Yuehua Wei,Yi Eve Sun,Yinan Zhang,Congrong Wang

Journal of veterinary science 19:528-535 PubMed29649860

2018

Identification of determinants that mediate binding between Tembusu virus and the cellular receptor heat shock protein A9.

Applications

Unspecified application

Species

Unspecified reactive species

Dongmin Zhao,Qingtao Liu,Xinmei Huang,Huili Wang,Kaikai Han,Jing Yang,Keran Bi,Yuzhuo Liu,Lijiao Zhang,Yin Li

Molecular medicine reports 16:8595-8604 PubMed29039499

2017

An investigation of methyl tert‑butyl ether‑induced cytotoxicity and protein profile in Chinese hamster ovary cells.

Applications

Unspecified application

Species

Unspecified reactive species

Guangshan Xie,Wen-Xu Hong,Li Zhou,Xifei Yang,Haiyan Huang,Desheng Wu,Xinfeng Huang,Weiguo Zhu,Jianjun Liu

PloS one 11:e0150357 PubMed26959487

2016

Endoplasmic Reticulum Stress and Autophagy in Homocystinuria Patients with Remethylation Defects.

Applications

Species

Unspecified reactive species

Ainhoa Martínez-Pizarro,Lourdes R Desviat,Magdalena Ugarte,Belén Pérez,Eva Richard

제품이 사용된 논문 모두 보기

style

left-column

style

left-column

style

right-column

Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Grp75/MOT antibody [9F8]

Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (AB171089)

Immunocytochemistry/ Immunofluorescence - Anti-Grp75/MOT antibody [9F8] (AB171089)

Immunoprecipitation - Anti-Grp75/MOT antibody [9F8] (AB171089)

Western blot - Anti-Grp75/MOT antibody [9F8] (AB171089)

Western blot - Anti-Grp75/MOT antibody [9F8] (AB171089)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Primary Antibodies

Primary Antibodies

Reagents, Controls & Accessories

Primary Antibodies

Reactivity 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 기간

적절한 단기 보관 조건

적절한 장기 보관 조건

분주 정보

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

대체 명칭 보기

제품이 사용된 논문 (6)

Product promise