AB62368

Anti-GSK3 (alpha + beta) antibody [EP933Y]

20ul selling size
RabMAb
Recombinant
각 태그의 뜻

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(7 제품이 사용된 논문 )

Rabbit Recombinant Monoclonal GSK3 alpha antibody. Suitable for WB and reacts with Human samples. Cited in 7 publications.

1 이미지

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Western blot - Anti-GSK3 (alpha + beta) antibody [EP933Y] (AB62368)

All lanes:

Western blot - Anti-GSK3 (alpha + beta) antibody [EP933Y] (ab62368) at 1/2000 dilution

All lanes:

293T cell lysate at 10 µg

Secondary

All lanes:

goat anti-rabbit, HRP labeled, at 1/2000 dilution

Predicted band size: 51 kDa

Observed band size: 47 kDa

false

주요 정보

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP933Y

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Specificity

ab62368 detects both GSK3 alpha and GSK3 beta.

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Reactivity 정보

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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제품 세부 정보

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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특성 및 보관 정보

제형

Liquid

Purification 테크닉

Affinity purification Protein A

보관 버퍼

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.31% Sodium citrate, 0.175% Sodium chloride, 0.05% BSA, 0.0172% Ethylenediaminetetraacetic acid, disodium, dihydrate

배송 시 보관 조건

Conditional Ambient

적절한 단기 보관 조건

+4°C

적절한 장기 보관 조건

-20°C

보관 정보

Stable for 12 months at -20°C

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추가 정보

This supplementary information is collated from multiple sources and compiled automatically.

The GSK3 protein known also as glycogen synthase kinase 3 has two isoforms: GSK3 alpha and GSK3 beta. Both isoforms have distinct yet significant mechanical roles. GSK3 alpha with a molecular weight of approximately 51 kDa and GSK3 beta weighing about 47 kDa act as serine/threonine kinases. These proteins are expressed in various tissues including the brain pancreas and liver. GSK3 is involved in the regulation of numerous cellular processes by phosphorylating its substrates impacting various cellular activities.

Biological function summary

GSK3 is integral in multiple signaling pathways and participates in the regulation of inflammation cell cycle and apoptosis. As part of the larger complex of signaling proteins GSK3 impacts the modulation of transcription factors and influences cellular differentiation and proliferation. The protein's kinase activity is necessary for maintaining cellular homeostasis and ensuring proper response to external signals.

Pathways

GSK3 influences critical pathways like Wnt and PI3K/Akt. Within the Wnt pathway GSK3 targets important proteins such as β-catenin where it plays an important role in controlling gene expression related to cell growth. In the PI3K/Akt pathway GSK3 acts as an important regulatory protein affecting insulin signaling and energy metabolism. Interactions with other kinases and transcription factors allow GSK3 to integrate various signaling cues that dictate cellular responses.

GSK3 has associations with neurodegenerative diseases and diabetes. In Alzheimer's disease GSK3 is linked to the formation of tau protein tangles contributing to neuronal dysfunction. Moreover in the context of diabetes GSK3 is involved in the insulin signaling pathway where dysregulation can lead to insulin resistance. It interacts with proteins such as tau in neurodegeneration and insulin receptor substrates in diabetes showing its significant role in these pathological conditions.

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제품 프로토콜

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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타겟 정보

Constitutively active protein kinase that acts as a negative regulator in the hormonal control of glucose homeostasis, Wnt signaling and regulation of transcription factors and microtubules, by phosphorylating and inactivating glycogen synthase (GYS1 or GYS2), CTNNB1/beta-catenin, APC and AXIN1 (PubMed : 11749387, PubMed : 17478001, PubMed : 19366350). Requires primed phosphorylation of the majority of its substrates (PubMed : 11749387, PubMed : 17478001, PubMed : 19366350). Contributes to insulin regulation of glycogen synthesis by phosphorylating and inhibiting GYS1 activity and hence glycogen synthesis (PubMed : 11749387, PubMed : 17478001, PubMed : 19366350). Regulates glycogen metabolism in liver, but not in muscle (By similarity). May also mediate the development of insulin resistance by regulating activation of transcription factors (PubMed : 10868943, PubMed : 17478001). In Wnt signaling, regulates the level and transcriptional activity of nuclear CTNNB1/beta-catenin (PubMed : 17229088). Facilitates amyloid precursor protein (APP) processing and the generation of APP-derived amyloid plaques found in Alzheimer disease (PubMed : 12761548). May be involved in the regulation of replication in pancreatic beta-cells (By similarity). Is necessary for the establishment of neuronal polarity and axon outgrowth (By similarity). Through phosphorylation of the anti-apoptotic protein MCL1, may control cell apoptosis in response to growth factors deprivation (By similarity). Acts as a regulator of autophagy by mediating phosphorylation of KAT5/TIP60 under starvation conditions which activates KAT5/TIP60 acetyltransferase activity and promotes acetylation of key autophagy regulators, such as ULK1 and RUBCNL/Pacer (PubMed : 30704899). Negatively regulates extrinsic apoptotic signaling pathway via death domain receptors. Promotes the formation of an anti-apoptotic complex, made of DDX3X, BRIC2 and GSK3B, at death receptors, including TNFRSF10B. The anti-apoptotic function is most effective with weak apoptotic signals and can be overcome by stronger stimulation (By similarity). Phosphorylates mTORC2 complex component RICTOR at 'Thr-1695' which facilitates FBXW7-mediated ubiquitination and subsequent degradation of RICTOR (PubMed : 25897075).

See full target information Glycogen synthase kinase-3 alpha

Additional targets

GSK3B

대체 명칭 보기

Glycogen synthase kinase-3 alpha, GSK-3 alpha, Serine/threonine-protein kinase GSK3A, GSK3A, Glycogen synthase kinase-3 beta, GSK-3 beta, Serine/threonine-protein kinase GSK3B, GSK3B

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제품이 사용된 논문 (7)

Recent publications for all applications. Explore the 전체 목록 and refine your search

Stem cells translational medicine 14: PubMed39560969

2024

Tailoring cell therapies for diabetic metabolic phenotypes: a comparative study on the efficacy of various umbilical cord-derived cell regimens.

Applications

Unspecified application

Species

Unspecified reactive species

Lingshu Wang,Liming Wang,Falian He,Jia Song,Jingting Qiao,Jun Qin,Li Chen,Xinguo Hou

Stem cell research & therapy 11:223 PubMed32513303

2020

Mesenchymal stem cell-derived exosomes exert ameliorative effects in type 2 diabetes by improving hepatic glucose and lipid metabolism via enhancing autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Qin He,Lingshu Wang,Ruxing Zhao,Fei Yan,Sha Sha,Chen Cui,Jia Song,Huiqing Hu,Xinghong Guo,Mengmeng Yang,Yixin Cui,Yujing Sun,Zheng Sun,Fuqiang Liu,Ming Dong,Xinguo Hou,Li Chen

Stem cell research & therapy 10:270 PubMed31445515

2019

Mesenchymal stem cells restore the sperm motility from testicular torsion-detorsion injury by regulation of glucose metabolism in sperm.

Applications

Unspecified application

Species

Unspecified reactive species

Chi-Hao Hsiao,Andrea Tung-Qian Ji,Chih-Cheng Chang,Ming-Hsien Chien,Liang-Ming Lee,Jennifer Hui-Chun Ho

eLife 6: PubMed28440748

2017

Palmitoylated SCP1 is targeted to the plasma membrane and negatively regulates angiogenesis.

Applications

Species

Unspecified reactive species

Peng Liao,Weichao Wang,Yu Li,Rui Wang,Jiali Jin,Weijuan Pang,Yunfei Chen,Mingyue Shen,Xinbo Wang,Dongyang Jiang,Jinjiang Pang,Mingyao Liu,Xia Lin,Xin-Hua Feng,Ping Wang,Xin Ge

Cancer research 73:5742-53 PubMed23884910

2013

Akt SUMOylation regulates cell proliferation and tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Rong Li,Jie Wei,Cong Jiang,Dongmei Liu,Lu Deng,Kai Zhang,Ping Wang

Nature medicine 18:1643-50 PubMed23086477

2012

The sirtuin SIRT6 blocks IGF-Akt signaling and development of cardiac hypertrophy by targeting c-Jun.

Applications

Species

Mouse

Nagalingam R Sundaresan,Prabhakaran Vasudevan,Lei Zhong,Gene Kim,Sadhana Samant,Vishwas Parekh,Vinodkumar B Pillai,P V Ravindra,Madhu Gupta,Valluvan Jeevanandam,John M Cunningham,Chu-Xia Deng,David B Lombard,Raul Mostoslavsky,Mahesh P Gupta

Cancer research 72:4329-39 PubMed22777824

2012

Global quantitative phosphoproteome analysis of human tumor xenografts treated with a CD44 antagonist.

Applications

Unspecified application

Species

Unspecified reactive species

Stefan Weigand,Frank Herting,Daniela Maisel,Adam Nopora,Edgar Voss,Christoph Schaab,Martin Klammer,Andreas Tebbe

제품이 사용된 논문 모두 보기

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Product promise

저희는 고품질 시약으로 고객님의 연구를 서포트하고자 최선을 다하고 있으며, 모든 단계에서 함께 하겠습니다. 만약 제품이 기대한 성능을 보이지 않을 경우에도 Product Promise로 보호받으실 수 있습니다.

자세한 내용은 이용 약관을 확인해 주세요.

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-GSK3 (alpha + beta) antibody [EP933Y]

Western blot - Anti-GSK3 (alpha + beta) antibody [EP933Y] (AB62368)

관련 conjugated 항체와 다양한 조성의 항체 (1)

주요 정보

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

대체품

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

보완 제품

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity 정보

제품 세부 정보

특성 및 보관 정보

제형

Purification 테크닉

보관 버퍼

배송 시 보관 조건

적절한 단기 보관 조건

적절한 장기 보관 조건

보관 정보

추가 정보

Biological function summary

Pathways

제품 프로토콜

타겟 정보

Additional targets

대체 명칭 보기

제품이 사용된 논문 (7)

Product promise